uBE2S Recombinant Protein

Name: uBE2S Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203373
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203373

uBE2S, also known as ubiquitin-conjugating enzyme E2S, is a member of the ubiquitin-conjugating enzyme family. This protein is able to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme-dependent manner, a characteristic property of… More details

$210 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

uBE2S, Recombinant Protein

Full Product Name

uBE2S, 1-222aa, Human, His tag, E Coli

Product Gene Name

uBE2S recombinant protein

Product Synonym Names

ubiquitin-conjugating enzyme E2S; E2-EPF; E2EPF; EPF5; ubiquitin-conjugating enzyme E2S AA409170; MGC101982; uBE2S; ubiquitin carrier protein S; ubiquitin protein ligase S; ubiquitin conjugating enzyme E2 24 kD; ubiquitin conjugating enzyme E2S

Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNSN VENLPPHIIR LVYKEVTTLT ADPPDGIKVF PNEEDLTDLQ VTIEGPEGTP YAGGLFRMKL LLGKDFPASP PKGYFLTKIF HPNVGANGEI CVNVLKRDWT AELGIRHVLL TIKCLLIHPN PESALNEEAG RLLLENYEEY AARARLLTEI HGGAGGPSGR AEAGRALASG TEASSTDPGA PGGPGGAEGP MAKKHAGERD KKLAAKKKTD KKRALRRL

Host

E Coli

Form/Format

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT and 20% glycerol

Purity/Purification

> 90% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

27.9 kDa (258aa), confirmed by MALDI-TOF.

OMIM

M91670 mRNA

3D Structure

ModBase 3D Structure for Q16763

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for uBE2S recombinant protein

Ubiquitination

Applications Tested/Suitable for uBE2S recombinant protein

SDS-PAGE

Product References and Citations for uBE2S recombinant protein

Ye Y., et al. (2009) Nat Rev Mol Cell Biol. 10 (11):755-64. ; Windheim M., et al. (2008) Biochem J. 409 (3):723-9.;

Other Notes

Small volumes of uBE2S recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for uBE2S purchase

MBS203373 is a recombinant protein targeting uBE2S. Also known as uBE2S, 1-222aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for uBE2S. It may not necessarily be applicable to this product.

NCBI GI #

112382377

NCBI GeneID

27338

NCBI Accession #

NP_055316.2

UniProt Primary Accession #

Q16763

UniProt Secondary Accession #

Q9BTC1

UniProt Related Accession #

Q16763

GenBank Nucleotide #

NM_014501.2

NCBI Official Full Name

ubiquitin-conjugating enzyme E2 S

NCBI Official Synonym Full Names

ubiquitin conjugating enzyme E2 S

NCBI Official Symbol

UBE2S

NCBI Official Synonym Symbols

EPF5; E2EPF; E2-EPF

UniProt Protein Name

Ubiquitin-conjugating enzyme E2 S

UniProt Synonym Protein Names

E2 ubiquitin-conjugating enzyme S; E2-EPF; Ubiquitin carrier protein S; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-conjugating enzyme E2-EPF5; Ubiquitin-protein ligase S

Protein Family

Ubiquitin-conjugating enzyme

UniProt Gene Name

UBE2S

UniProt Synonym Gene Names

E2EPF

NCBI Protein Information

ubiquitin-conjugating enzyme E2 S

NCBI Summary for uBE2S

This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq, Jul 2008]

UniProt Comments for uBE2S

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:22496338). Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit (PubMed:19820702, PubMed:19822757, PubMed:27259151). Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A (PubMed:16819549). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination (PubMed:20061386, PubMed:20622874).

Research Articles on uBE2S

1. High UBE2S expression is associated with drug resistance in glioblastoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with uBE2S recombinant protein

Reference Product	PubMed Publications
CDC20 recombinant protein	>5 publications with uBE2S and CDC20
ANAPC11 recombinant protein	>4 publications with uBE2S and ANAPC11
CDC27 recombinant protein	>2 publications with uBE2S and CDC27
ANAPC15 recombinant protein	>1 publications with uBE2S and ANAPC15
CDC23 recombinant protein	>1 publications with uBE2S and CDC23
UBC recombinant protein	>1 publications with uBE2S and UBC

Pathways associated with uBE2S recombinant protein

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies	Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies	Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Post-translational Protein Modification Pathway antibodies	Post-translational Protein Modification Pathway Diagram
Protein Ubiquitination Pathway antibodies	Protein Ubiquitination Pathway Diagram
Synthesis Of Active Ubiquitin: Roles Of E1 And E2 Enzymes Pathway antibodies	Synthesis Of Active Ubiquitin: Roles Of E1 And E2 Enzymes Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram

Diseases associated with uBE2S recombinant protein

Disease Name	Pubmed Publications
Liver Neoplasms Antibodies	>2 publications with uBE2S and Liver Neoplasms
Neoplasm Invasiveness Antibodies	>2 publications with uBE2S and Neoplasm Invasiveness
Lung Diseases Antibodies	>1 publications with uBE2S and Lung Diseases
Kidney Diseases Antibodies	>1 publications with uBE2S and Kidney Diseases
Lung Neoplasms Antibodies	>1 publications with uBE2S and Lung Neoplasms
Carcinoma, Hepatocellular Antibodies	>1 publications with uBE2S and Carcinoma, Hepatocellular
Liver Diseases Antibodies	>1 publications with uBE2S and Liver Diseases
Cell Transformation, Neoplastic Antibodies	>1 publications with uBE2S and Cell Transformation, Neoplastic

Organs/Tissues associated with uBE2S recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>3 publications with uBE2S and Brain
Liver Antibodies	>2 publications with uBE2S and Liver
Blood Antibodies	>1 publications with uBE2S and Blood
Kidney Antibodies	>1 publications with uBE2S and Kidney
Lung Antibodies	>1 publications with uBE2S and Lung