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CDC42 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203396

Cell division cycle 42 isoform 1, also known as CDC42, is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. Also, this… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CDC42, Recombinant Protein
Full Product Name
CDC42, 1- 188aa, Human, T7-tag, E Coli
Product Gene Name
CDC42 recombinant protein
Product Synonym Names
Cell division cycle 42 isoform 1; G25K; Cell division cycle 42 isoform 1 CDC42Hs; Cell division cycle 42; Small GTP binding protein CDC42.
Sequence
MASMTGGQQM GRGSHMQTIK CVVVGDGAVG KTCLLISYTT NKFPSEYVPT VFDNYAVTVM IGGEPYTLGL FDTAGQEDYD RLRPLSYPQT DVFLVCFSVV SPSSFENVKE KWVPEITHHC PKTPFLLVGT QIDLRDDPST IEKLAKNKQK PITPETAEKL ARDLKAVKYV ECSALTQKGL KNVFDEAILA ALEPPEPKKS RRC
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 2mM EDTA
Purity/Purification
> 95% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
22.4 kDa (203aa), confirmed by MALDI-TOF.
OMIM
M35543 mRNA
3D Structure
ModBase 3D Structure for P60953
Antigen Species
Human
Tag Information
T7 tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Product Categories/Family for CDC42 recombinant protein

Signal Transduction
Applications Tested/Suitable for CDC42 recombinant protein
SDS-PAGE

SDS-Page of CDC42 recombinant protein

CDC42 Recombinant Protein – Cat# MBS203396

Product References and Citations for CDC42 recombinant protein

Kathryn P., et al. (2008) J Cell Biol. 183 (6):1129-1143. ; Li R., et al. (1997) J Biol Chem. 272 (52):32830-5. ;

Other Notes

Small volumes of CDC42 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDC42 purchase

Small GTP binding proteins (small GTPase family of Rho like signaling) include cdc42 protein. Mouse or human cdc42 regulates cell division cycle steps by regulation of actin. For cdc assay development, having species specific cdc42 recombinant protein generated by e coli expression is critical. Our specific species human mouse rabbit or rat cdc 42 GTP binding protein is ideal for generation of antibody reagents for ELISA assays as both mouse monoclonal species and rabbit polyclonal species antibodies can be created. Our human cdc42 protein is available with a T7-tag and size verified to protein 25kD size by western blot. Alternative experimental uses include IHC antibody neutralization studies or stem cell differentiation protocols. This product is available for shipping worldwide select country of origin: United States, United Kingdom, Czech Republic Australia Austria Azerbaijan Bahamas Belarus Belgium Belize and Ecuador Egypt el. Find us at MyBiosource.com for all your assay kits needs.

Additional Details

NCBI/Uniprot data below describe general gene information for CDC42. It may not necessarily be applicable to this product.

NCBI GI #
4757952
NCBI GeneID
998
NCBI Accession #
NP_001782
UniProt Primary Accession #
P60953
UniProt Secondary Accession #
P21181; P25763; Q7L8R5; Q9UDI2
UniProt Related Accession #
P60953
GenBank Nucleotide #
NM_001791.3
NCBI Official Full Name
cell division control protein 42 homolog isoform 1
NCBI Official Synonym Full Names
cell division cycle 42
NCBI Official Symbol
CDC42
NCBI Official Synonym Symbols
TKS; G25K; CDC42Hs
UniProt Protein Name
Cell division control protein 42 homolog
UniProt Synonym Protein Names
G25K GTP-binding protein
Protein Family
Cdc42
UniProt Gene Name
CDC42
NCBI Protein Information
cell division control protein 42 homolog

NCBI Summary for CDC42

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]
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UniProt Comments for CDC42

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration. Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. Facilitates filopodia formation upon DOCK11-activation (). Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups.
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Research Articles on CDC42

1. our data identify Epsin2 as a novel player in regulating oocyte maturation, and demonstrate that Epsin2 promotes polarity establishment and meiotic division via activating Cdc42

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CDC42 recombinant protein

 Reference Product  PubMed Publications
 PAK1 recombinant protein  >354 publications with CDC42 and PAK1
 IQGAP1 recombinant protein  >134 publications with CDC42 and IQGAP1
 PAK2 recombinant protein  >100 publications with CDC42 and PAK2
 PAK3 recombinant protein  >54 publications with CDC42 and PAK3
 ARHGDIA recombinant protein  >33 publications with CDC42 and ARHGDIA
 PAK7 recombinant protein  >21 publications with CDC42 and PAK7
 PAK6 recombinant protein  >18 publications with CDC42 and PAK6
 PARD6A recombinant protein  >15 publications with CDC42 and PARD6A
 ARHGAP1 recombinant protein  >10 publications with CDC42 and ARHGAP1
 PIK3CB recombinant protein  >10 publications with CDC42 and PIK3CB

Pathways associated with CDC42 recombinant protein

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Diseases associated with CDC42 recombinant protein

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >310 publications with CDC42 and Carcinoma
 Inflammation Antibodies  >169 publications with CDC42 and Inflammation
 Breast Neoplasms Antibodies  >168 publications with CDC42 and Breast Neoplasms
 Disease Models, Animal Antibodies  >147 publications with CDC42 and Disease Models, Animal
 Lung Diseases Antibodies  >112 publications with CDC42 and Lung Diseases
 Necrosis Antibodies  >108 publications with CDC42 and Necrosis
 Cardiovascular Diseases Antibodies  >105 publications with CDC42 and Cardiovascular Diseases
 Lung Neoplasms Antibodies  >98 publications with CDC42 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >71 publications with CDC42 and Neoplasms, Experimental
 Liver Diseases Antibodies  >70 publications with CDC42 and Liver Diseases

Organs/Tissues associated with CDC42 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >704 publications with CDC42 and Blood
 Brain Antibodies  >581 publications with CDC42 and Brain
 Vascular Antibodies  >376 publications with CDC42 and Vascular
 Nerve Antibodies  >356 publications with CDC42 and Nerve
 Muscle Antibodies  >269 publications with CDC42 and Muscle
 Kidney Antibodies  >255 publications with CDC42 and Kidney
 Lung Antibodies  >245 publications with CDC42 and Lung
 Embryonic Tissue Antibodies  >190 publications with CDC42 and Embryonic Tissue
 Bone Antibodies  >189 publications with CDC42 and Bone
 Liver Antibodies  >158 publications with CDC42 and Liver
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