3ug by SDS-PAGE under reducing condition and realized by coomassie blue stain.

EIF4E Recombinant Protein

Name: EIF4E Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203448
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203448

EIF4E, a member of eukaryotic initiation factor 4 families, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby… More details

$245 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

EIF4E, Recombinant Protein

Full Product Name

EIF4E, 1-217aa, Human, His tag, E Coli

Product Gene Name

EIF4E recombinant protein

Product Synonym Names

Eukaryotic translation initiation factor 4E; CBP; EIF4E1; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E

Sequence

MGSSHHHHHH SSGLVPRGSH MATVEPETTP TPNPPTTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV

Host

E Coli

Form/Format

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Purity/Purification

> 85% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 2-8°C for 1 week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

27.2 kDa (237aa), confirmed by MALDI-TOF

OMIM

M15353 mRNA

3D Structure

ModBase 3D Structure for P06730

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for EIF4E recombinant protein

Transcription & Translation

Applications Tested/Suitable for EIF4E recombinant protein

SDS-PAGE

Product References and Citations for EIF4E recombinant protein

Bendahmane A., et al. (2010) PLoS One. 5 (6):e11313.; Pelletier J., et al. (2010) J Gen Virol. 91 (Pt 5):1224-8.;

Other Notes

Small volumes of EIF4E recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EIF4E purchase

MBS203448 is a recombinant protein targeting EIF4E. Also known as EIF4E, 1-217aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF4E. It may not necessarily be applicable to this product.

NCBI GI #

4503535

NCBI GeneID

1977

NCBI Accession #

NP_001959

UniProt Primary Accession #

P06730

UniProt Secondary Accession #

Q96E95; B7Z6V1; D6RCQ6

UniProt Related Accession #

P06730

GenBank Nucleotide #

NM_001968.4

NCBI Official Full Name

eukaryotic translation initiation factor 4E isoform 1

NCBI Official Synonym Full Names

eukaryotic translation initiation factor 4E

NCBI Official Symbol

EIF4E

NCBI Official Synonym Symbols

CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1

UniProt Protein Name

Eukaryotic translation initiation factor 4E

UniProt Synonym Protein Names

eIF-4F 25 kDa subunit; mRNA cap-binding protein

Protein Family

Eukaryotic translation initiation factor

UniProt Gene Name

EIF4E

UniProt Synonym Gene Names

EIF4EL1; EIF4F; eIF-4E; eIF4E

NCBI Protein Information

eukaryotic translation initiation factor 4E

NCBI Summary for EIF4E

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

UniProt Comments for EIF4E

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Research Articles on EIF4E

1. EIF4E associated signaling pathways are associated with lymphangiogenesis and lymph node metastases of hypopharyngeal cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with EIF4E recombinant protein

Products by Pathway	Pathway Diagram
4-hydroxytamoxifen, Dexamethasone, And Retinoic Acids Regulation Of P27 Expression Pathway antibodies	4-hydroxytamoxifen, Dexamethasone, And Retinoic Acids Regulation Of P27 Expression Pathway Diagram
Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies	Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
Angiopoietin Like Protein 8 Regulatory Pathway antibodies	Angiopoietin Like Protein 8 Regulatory Pathway Diagram
Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies	Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
Brain-Derived Neurotrophic Factor (BDNF) Signaling Pathway antibodies	Brain-Derived Neurotrophic Factor (BDNF) Signaling Pathway Diagram
Cap-dependent Translation Initiation Pathway antibodies	Cap-dependent Translation Initiation Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Deadenylation Of MRNA Pathway antibodies	Deadenylation Of MRNA Pathway Diagram
Deadenylation-dependent MRNA Decay Pathway antibodies	Deadenylation-dependent MRNA Decay Pathway Diagram
EGFR Tyrosine Kinase Inhibitor Resistance Pathway antibodies	EGFR Tyrosine Kinase Inhibitor Resistance Pathway Diagram

Diseases associated with EIF4E recombinant protein

Disease Name	Pubmed Publications
Cell Transformation, Neoplastic Antibodies	>130 publications with EIF4E and Cell Transformation, Neoplastic
Adenocarcinoma Antibodies	>119 publications with EIF4E and Adenocarcinoma
Necrosis Antibodies	>79 publications with EIF4E and Necrosis
Cardiovascular Diseases Antibodies	>77 publications with EIF4E and Cardiovascular Diseases
Lung Neoplasms Antibodies	>73 publications with EIF4E and Lung Neoplasms
Neoplasm Metastasis Antibodies	>72 publications with EIF4E and Neoplasm Metastasis
Hypertrophy Antibodies	>71 publications with EIF4E and Hypertrophy
Neoplasms, Experimental Antibodies	>66 publications with EIF4E and Neoplasms, Experimental
Inflammation Antibodies	>63 publications with EIF4E and Inflammation
Liver Diseases Antibodies	>63 publications with EIF4E and Liver Diseases

Organs/Tissues associated with EIF4E recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>199 publications with EIF4E and Brain
Lung Antibodies	>127 publications with EIF4E and Lung
Uterus Antibodies	>16 publications with EIF4E and Uterus