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SMAD3 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203450

SMAD3, also known as mothers against decapentaplegic homolog 3, belongs to the SMAD family of proteins that mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the… More details

$220 / 0.02 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SMAD3, Recombinant Protein
Full Product Name
SMAD3, 1-425aa, Human, His tag, E Coli
Product Gene Name
SMAD3 recombinant protein
Product Synonym Names
Mothers against decapentaplegic homolog 3; HSPC193; HsT17436; JV15-2; MADH3; Mothers against decapentaplegic homolog 3 DKFZP586N0721; DKFZp686J10186; hMAD 3; hSMAD3; MAD (mothers against decapentaplegic Drosophila) homolog 3; HST17436; JV15 2; JV152; MAD3; MADH 3; MGC60396; Mothers against DPP homolog 3; SMA and MAD related protein 3; SMAD; SMAD 3; SMAD3
Sequence
MGSSHHHHHH SSGLVPRGSH MSSILPFTPP IVKRLLGWKK GEQNGQEEKW CEKAVKSLVK KLKKTGQLDE LEKAITTQNV NTKCITIPRS LDGRLQVSHR KGLPHVIYCR LWRWPDLHSH HELRAMELCE FAFNMKKDEV CVNPYHYQRV ETPVLPPVLV PRHTEIPAEF PPLDDYSHSI PENTNFPAGI EPQSNIPETP PPGYLSEDGE TSDHQMNHSM DAGSPNLSPN PMSPAHNNLD LQPVTYCEPA FWCSISYYEL NQRVGETFHA SQPSMTVDGF TDPSNSERFC LGLLSNVNRN AAVELTRRHI GRGVRLYYIG GEVFAECLSD SAIFVQSPNC NQRYGWHPAT VCKIPPGCNL KIFNNQEFAA LLAQSVNQGF EAVYQLTRMC TIRMSFVKGW GAEYRRQTVT STPCWIELHL NGPLQWLDKV LTQMGSPSIR CSSVS
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl and 1 mM DTT
Purity/Purification
> 90% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
50.2 kDa (445aa), confirmed by MALDI-TOF.
OMIM
U68019 mRNA
3D Structure
ModBase 3D Structure for P84022
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for SMAD3 recombinant protein

Transcription & Translation
Applications Tested/Suitable for SMAD3 recombinant protein
SDS-PAGE

SDS-Page of SMAD3 recombinant protein

SMAD3 Recombinant Protein – Cat# MBS203450

Product References and Citations for SMAD3 recombinant protein

Zhang Y., et al. (1996) Nature. 383 (6596):168-72.; Wang H., et al. (2005) J Biol Chem. 280 (7):5154-62.;

Other Notes

Small volumes of SMAD3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMAD3 purchase

MBS203450 is a recombinant protein targeting SMAD3. Also known as SMAD3, 1-425aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for SMAD3. It may not necessarily be applicable to this product.

NCBI GI #
5174513
NCBI GeneID
4088
NCBI Accession #
NP_005893
UniProt Primary Accession #
P84022
UniProt Secondary Accession #
O09064; O09144; O14510; O35273; Q92940; Q93002; A8K4B6; B7Z4Z5; B7Z6M9; B7Z9Q2; F5H383
UniProt Related Accession #
P84022
GenBank Nucleotide #
NM_005902.3
NCBI Official Full Name
mothers against decapentaplegic homolog 3 isoform 1
NCBI Official Synonym Full Names
SMAD family member 3
NCBI Official Symbol
SMAD3
NCBI Official Synonym Symbols
LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
UniProt Protein Name
Mothers against decapentaplegic homolog 3
UniProt Synonym Protein Names
JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Protein Family
Mothers against decapentaplegic
UniProt Gene Name
SMAD3
UniProt Synonym Gene Names
MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3
NCBI Protein Information
mothers against decapentaplegic homolog 3

NCBI Summary for SMAD3

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
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UniProt Comments for SMAD3

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
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Research Articles on SMAD3

1. Authors were able to confirm expression of SMAD3 in intact and degraded cartilage of the knee and hip. Our findings provide the first systematic evaluation of pleiotropy between OA and BMD, highlight genes with biological relevance to both traits, and establish a robust new OA genetic risk locus at SMAD3.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SMAD3 recombinant protein

 Reference Product  PubMed Publications
 TGFB1 recombinant protein  >1862 publications with SMAD3 and TGFB1
 JUN recombinant protein  >628 publications with SMAD3 and JUN
 TGFBR1 recombinant protein  >64 publications with SMAD3 and TGFBR1
 RUNX2 recombinant protein  >56 publications with SMAD3 and RUNX2
 SMURF2 recombinant protein  >46 publications with SMAD3 and SMURF2
 ACVR1B recombinant protein  >27 publications with SMAD3 and ACVR1B
 FOXH1 recombinant protein  >26 publications with SMAD3 and FOXH1
 SMURF1 recombinant protein  >18 publications with SMAD3 and SMURF1
 MAPK1 recombinant protein  >14 publications with SMAD3 and MAPK1
 FOXO3 recombinant protein  >12 publications with SMAD3 and FOXO3

Pathways associated with SMAD3 recombinant protein

 Products by Pathway  Pathway Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Activin Signaling Pathway antibodies  Activin Signaling Pathway Diagram
 Activin Signaling Pathway antibodies  Activin Signaling Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Apelin Signaling Pathway antibodies  Apelin Signaling Pathway Diagram

Diseases associated with SMAD3 recombinant protein

 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >1466 publications with SMAD3 and Fibrosis
 Disease Models, Animal Antibodies  >548 publications with SMAD3 and Disease Models, Animal
 Inflammation Antibodies  >536 publications with SMAD3 and Inflammation
 Liver Diseases Antibodies  >357 publications with SMAD3 and Liver Diseases
 Kidney Diseases Antibodies  >337 publications with SMAD3 and Kidney Diseases
 Heart Diseases Antibodies  >223 publications with SMAD3 and Heart Diseases
 Liver Cirrhosis Antibodies  >203 publications with SMAD3 and Liver Cirrhosis
 Necrosis Antibodies  >181 publications with SMAD3 and Necrosis
 Breast Neoplasms Antibodies  >171 publications with SMAD3 and Breast Neoplasms
 Neoplasm Invasiveness Antibodies  >138 publications with SMAD3 and Neoplasm Invasiveness

Organs/Tissues associated with SMAD3 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >978 publications with SMAD3 and Muscle
 Blood Antibodies  >637 publications with SMAD3 and Blood
 Lung Antibodies  >589 publications with SMAD3 and Lung
 Kidney Antibodies  >570 publications with SMAD3 and Kidney
 Bone Antibodies  >533 publications with SMAD3 and Bone
 Liver Antibodies  >525 publications with SMAD3 and Liver
 Vascular Antibodies  >453 publications with SMAD3 and Vascular
 Connective Tissue Antibodies  >438 publications with SMAD3 and Connective Tissue
 Heart Antibodies  >355 publications with SMAD3 and Heart
 Skin Antibodies  >313 publications with SMAD3 and Skin
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