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SKP Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203478

The Seventeen Kilodalton Protein (Skp) is a trimeric periplasmic chaperone that assists outer membrane proteins in their folding and insertion into membranes. This protein required for the efficient release of ompA from the inner membrane, the maintenance of… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Skp, Recombinant Protein
Full Product Name
Skp, 21-161aa, E Coli, His tag, E Coli
Product Gene Name
Product Synonym Names
Chaperone protein skp; Chaperone protein skp; periplasmic chaperone; ompH; DNA binding 17 kDa protein; ECK0177; Histone like protein HLP 1; hlpA; JW0173; Seventeen kilodalton protein
Sequence
MGSSHHHHHH SSGLVPRGSH MADKIAIVNM GSLFQQVAQK TGVSNTLENE FKGRASELQR METDLQAKMK KLQSMKAGSD RTKLEKDVMA QRQTFAQKAQ AFEQDRARRS NEERGKLVTR IQTAVKSVAN SQDIDLVVDA NAVAYNSSDV KDITADVLKQ VK
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
1mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 2-8°C for 1 week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
17.9 kDa (162aa), confirmed by MALDI-TOF
3D Structure
ModBase 3D Structure for P0AEU7
Antigen Species
E Coli
Tag Information
His-tag
Domain
21-161aa

Product Categories/Family for Skp recombinant protein

Applications Tested/Suitable for Skp recombinant protein
SDS-PAGE

SDS-Page of Skp recombinant protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
SKP Recombinant Protein – Cat# MBS203478

Product References and Citations for Skp recombinant protein

Korndorfer IP., et al. (2004) Nat Struct Mol Biol. 11 (10):1015-20; Walton TA., et al. (2004) Mol Cell. 15 (3):367-74.

Other Notes

Small volumes of Skp recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Skp purchase

MBS203478 is a recombinant protein targeting Skp. Also known as Skp, 21-161aa, E Coli, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for Skp. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
periplasmic chaperone
NCBI Official Symbol
skp
NCBI Official Synonym Symbols
ECK0177; hlpA; JW0173; ompH
UniProt Protein Name
Chaperone protein Skp
UniProt Synonym Protein Names
DNA-binding 17 kDa protein; Histone-like protein HLP-1; Seventeen kilodalton protein
Protein Family
UniProt Gene Name
skp
UniProt Synonym Gene Names
hlpA; ompH
NCBI Protein Information
periplasmic chaperone

NCBI Summary for Skp

The reported binding to DNA was an artifact due to the cationic nature of Skp. [More information is available at EcoGene: EG10455]. Skp is a periplasmic protein chaperone that binds to unfolded outer membrane proteins. [More information is available at EcoCyc: EG10455].

UniProt Comments for Skp

Molecular chaperone that interacts specifically with outer membrane proteins, thus maintaining the solubility of early folding intermediates during passage through the periplasm. Required for the efficient release of OmpA from the inner membrane, the maintenance of its solubility in the periplasm, and, in association with lipopolysaccharide (LPS), for the efficient folding and insertion of OmpA into the outer membrane.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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