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DDT Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203497

DDT is an enzyme that catayzes the tautomerization of D-dopachrome to give 5,6-dihydroxyindole (DHI). This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DDT, Recombinant Protein
Full Product Name
DDT, 1-118aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
D-dopachrome tautomerase; DDCT; D-dopachtome decarboxylase; D-dopachrome tautomerase; D dopachrome decarboxylase; D dopachrome tautomerase; Phenylpyruvate tautomerase II
Sequence
MGSSHHHHHH SSGLVPRGSH MPFLELDTNL PANRVPAGLE KRLCAAAASI LGKPADRVNV TVRPGLAMAL SGSTEPCAQL SISSIGVVGT AEDNRSHSAH FFEFLTKELA LGQDRILIRF FPLESWQIGK IGTVMTFL
Host
E Coli
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
Molecular Weight
14.8 kDa (138aa), confirmed by MALDI-TOF
OMIM
U49785 mRNA
3D Structure
ModBase 3D Structure for P30046
Tag Information
His-tag
Domain
1-118aa

Product Categories/Family for DDT recombinant protein

Applications Tested/Suitable for DDT recombinant protein
SDS-PAGE

SDS-Page of DDT recombinant protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
DDT Recombinant Protein – Cat# MBS203497

Product References and Citations for DDT recombinant protein

Arisawa K., et al. (2009) Toxicology. 255 (1-2):6-14.; Nishihira J., et al. (1999) Biochemistry. 38 (11):3268-79.;

Other Notes

Small volumes of DDT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DDT purchase

MBS203497 is a recombinant protein targeting DDT. Also known as DDT, 1-118aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for DDT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
D-dopachrome decarboxylase
NCBI Official Synonym Full Names
D-dopachrome tautomerase
NCBI Official Symbol
DDT
NCBI Official Synonym Symbols
DDCT
UniProt Protein Name
D-dopachrome decarboxylase
UniProt Synonym Protein Names
D-dopachrome tautomerase; Phenylpyruvate tautomerase II
UniProt Gene Name
DDT
NCBI Protein Information
D-dopachrome decarboxylase

NCBI Summary for DDT

D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq, Jul 2008]

UniProt Comments for DDT

Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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