SHH Recombinant Protein

Name: SHH Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203523
Price: 220 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203523

SHH is one of three proteins in the mammalian signaling pathway family called hedgehog, the others being desert hedgehog (DHH) and Indian hedgehog (IHH). This protien is the best studied ligand of the hedgehog signaling pathway. It plays a key role in… More details

$220 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SHH, Recombinant Protein

Full Product Name

SHH, 25-198aa, Mouse, His tag, E Coli

Product Gene Name

SHH recombinant protein

Product Synonym Names

Sonic hedgehog protein; Dsh; Hhg1; Hx; Hx13; Sonic hedgehog protein HHG 1; HHG1; HLP 3; HLP3; Holoprosencephaly 3; HPE 3; HPE3; MCOPCB5; SHH; SMMC I; SMMCI; Sonic Hedgehog (Drosophila) homolog; sonic hedgehog homolog (Drosophila); Sonic hedgehog homolog; TPT; TPTPS.

Sequence

MCGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKITRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGGLEHHH HHH

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT.

Purity/Purification

> 95% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

20.8 kDa (183aa), confirmed by MALDI-TOF

3D Structure

ModBase 3D Structure for Q62226

Antigen Species

Human

Tag Information

His-tag

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Product Categories/Family for SHH recombinant protein

Development

Applications Tested/Suitable for SHH recombinant protein

SDS-PAGE

Product References and Citations for SHH recombinant protein

Bak M., et al. (2004) Brain Res Mol Brain Res. 126 (2):207-11. ; Dassule HR., et al. (2000) Development. 127 (22):4775-85. ;

Other Notes

Small volumes of SHH recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SHH purchase

MBS203523 is a recombinant protein targeting SHH. Also known as SHH, 25-198aa, Mouse, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for SHH. It may not necessarily be applicable to this product.

NCBI GI #

21617861

NCBI GeneID

20423

NCBI Accession #

NP_033196

UniProt Primary Accession #

Q62226

UniProt Related Accession #

Q62226

GenBank Nucleotide #

NM_009170.3

NCBI Official Full Name

sonic hedgehog protein

NCBI Official Synonym Full Names

sonic hedgehog

NCBI Official Symbol

Shh

NCBI Official Synonym Symbols

Hx; Dsh; Hhg1; Hxl3; ShhNC; M100081; 9530036O11Rik

UniProt Protein Name

Sonic hedgehog protein

UniProt Synonym Protein Names

Sonic hedgehog protein N-product; ShhN

Protein Family

Sonic hedgehog protein

UniProt Gene Name

Shh

UniProt Synonym Gene Names

ShhNp

NCBI Protein Information

sonic hedgehog protein

UniProt Comments for SHH

Sonic hedgehog protein: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (PubMed:8824192, PubMed:7891723). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (PubMed:8824192). Both activities occur in the reticulum endoplasmic (PubMed:21357747). Once cleaved, ShhC is degraded in the endoplasmic reticulum (PubMed:21357747).

Research Articles on SHH

1. smaller mvShh conjugates resulted in faster wound resolution compared to the unconjugated Shh. This study is the first to show how the wound healing efficacy of multivalent protein-polymer conjugates is sensitive to the polymer MW, and our findings suggest that this parameter could be used to enhance the efficacy of growth factor conjugates.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SHH recombinant protein

Reference Product	PubMed Publications
Smo recombinant protein	>562 publications with SHH and Smo
Ptch1 recombinant protein	>483 publications with SHH and Ptch1
Gli2 recombinant protein	>372 publications with SHH and Gli2
Hhip recombinant protein	>54 publications with SHH and Hhip
Ptch2 recombinant protein	>43 publications with SHH and Ptch2
Gas1 recombinant protein	>35 publications with SHH and Gas1
Cdon recombinant protein	>34 publications with SHH and Cdon
Boc recombinant protein	>32 publications with SHH and Boc
Gpc5 recombinant protein	>3 publications with SHH and Gpc5
Disp2 recombinant protein	>2 publications with SHH and Disp2

Pathways associated with SHH recombinant protein

Products by Pathway	Pathway Diagram
Activation Of SMO Pathway antibodies	Activation Of SMO Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
BMP Signaling Pathway In Eyelid Development antibodies	BMP Signaling Pathway In Eyelid Development Diagram
Basal Cell Carcinoma Pathway antibodies	Basal Cell Carcinoma Pathway Diagram
Basal Cell Carcinoma Pathway antibodies	Basal Cell Carcinoma Pathway Diagram
Dopaminergic Neurogenesis Pathway antibodies	Dopaminergic Neurogenesis Pathway Diagram
EDA Signalling In Hair Follicle Development Pathway antibodies	EDA Signalling In Hair Follicle Development Pathway Diagram
Heart Development Pathway antibodies	Heart Development Pathway Diagram
Hedgehog 'on' State Pathway antibodies	Hedgehog 'on' State Pathway Diagram

Diseases associated with SHH recombinant protein

Disease Name	Pubmed Publications
Abnormalities, Multiple Antibodies	>369 publications with SHH and Abnormalities, Multiple
Disease Models, Animal Antibodies	>323 publications with SHH and Disease Models, Animal
Craniofacial Abnormalities Antibodies	>270 publications with SHH and Craniofacial Abnormalities
Holoprosencephaly Antibodies	>222 publications with SHH and Holoprosencephaly
Adenocarcinoma Antibodies	>183 publications with SHH and Adenocarcinoma
Neural Tube Defects Antibodies	>119 publications with SHH and Neural Tube Defects
Cell Transformation, Neoplastic Antibodies	>118 publications with SHH and Cell Transformation, Neoplastic
Inflammation Antibodies	>104 publications with SHH and Inflammation
Lung Diseases Antibodies	>100 publications with SHH and Lung Diseases
Neoplasms, Experimental Antibodies	>96 publications with SHH and Neoplasms, Experimental

Organs/Tissues associated with SHH recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>1385 publications with SHH and Brain
Embryonic Tissue Antibodies	>1087 publications with SHH and Embryonic Tissue
Skin Antibodies	>313 publications with SHH and Skin
Lung Antibodies	>263 publications with SHH and Lung
Liver Antibodies	>162 publications with SHH and Liver
Prostate Antibodies	>108 publications with SHH and Prostate
Molar Antibodies	>56 publications with SHH and Molar
Bladder Antibodies	>54 publications with SHH and Bladder
Adrenal Gland Antibodies	>28 publications with SHH and Adrenal Gland