MDH2 Active Protein

Name: MDH2 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203531
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203531

MDH2 is one of the two malate dehydrogenases. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, MDH2 is localized to the mitochondria and may play… More details

$230 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MDH2, Active Protein

Full Product Name

MDH2, 25-338aa, Human, His tag, E Coli (Bioactivity Validated)

Product Gene Name

MDH2 active protein

Product Synonym Names

Malate dehydrogenase 2 NAD (mitochondrial); M-MDH; MDH; MOR1; Malate dehydrogenase 2; NAD (mitochondrial)

Sequence

MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET KAAVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMICVIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL TALTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVKSQET ECTYFSTPLL LGKKGIEKNL GIGKVSSFEE KMISDAIPEL KASIKKGEDF VKTLK

Host

E Coli

Form/Format

Liquid. In 20 mM Tris-HCl Buffer (pH 7.5) containing 10% glycerol

Purity/Purification

> 95% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

35.2 kDa (335aa), confirmed by MALDI-TOF

OMIM

AF047470 mRNA

3D Structure

ModBase 3D Structure for P40926

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Human

Tag Information

His-tag

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity

Specific activity is > 700 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Assay

1. Prepare a 180ul assay buffer into a suitable container. The concentrations are 100 mM potassium phosphate (pH8.0), 0.5mM beta-nicotinamide adenine dinucleotide, reduced form, 0.5mM oxalacetic acid.
2. Equilibrate to 37C and monitor at A340nm until the value is constant using a spectrophotometer.
3. Add 20ul of recombinant MDH2 protein 0.625ug/ml in assay buffer.
4. Mix by inversion and record the decrease at A340nm for approximately 5 minutes.

Product Categories/Family for MDH2 active protein

Signal Transduction

Applications Tested/Suitable for MDH2 active protein

SDS-PAGE

Product References and Citations for MDH2 active protein

Thummel CS., et al. (2010) Dev Dyn. 239 (3):954-64; Lee JS., et al (2002) J Basic Microbiol. 42 (4):238-45.;

Other Notes

Small volumes of MDH2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MDH2 purchase

MBS203531 is an active protein targeting MDH2. Also known as MDH2, 25-338aa, Human, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for MDH2. It may not necessarily be applicable to this product.

NCBI GI #

21735621

NCBI GeneID

4191

NCBI Accession #

NP_005909

UniProt Primary Accession #

P40926

UniProt Secondary Accession #

O43682; A8K414; B2RE78; B4DE44; E9PDB2

UniProt Related Accession #

P40926

GenBank Nucleotide #

NM_005918.3

NCBI Official Full Name

malate dehydrogenase, mitochondrial isoform 1

NCBI Official Synonym Full Names

malate dehydrogenase 2

NCBI Official Symbol

MDH2

NCBI Official Synonym Symbols

MDH; MOR1; M-MDH; EIEE51; MGC:3559

UniProt Protein Name

Malate dehydrogenase, mitochondrial

Protein Family

Malate dehydrogenase

UniProt Gene Name

MDH2

NCBI Protein Information

malate dehydrogenase, mitochondrial

NCBI Summary for MDH2

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

Research Articles on MDH2

1. loss-of-function mutations in MDH2 are also associated with severe neurological clinical presentations in children

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MDH2 active protein

Reference Product	PubMed Publications
MDH1 active protein	>42 publications with MDH2 and MDH1
GOT2 active protein	>7 publications with MDH2 and GOT2
ME1 active protein	>2 publications with MDH2 and ME1
GOT1 active protein	>2 publications with MDH2 and GOT1
ME2 active protein	>1 publications with MDH2 and ME2

Pathways associated with MDH2 active protein

Products by Pathway	Pathway Diagram
Amino Acid Metabolism Pathway antibodies	Amino Acid Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Citrate Cycle (TCA Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle) Pathway Diagram
Citrate Cycle (TCA Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle) Pathway Diagram
Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram
Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram
Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway antibodies	Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway Diagram
Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway antibodies	Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway Diagram
Citric Acid Cycle (TCA Cycle) Pathway antibodies	Citric Acid Cycle (TCA Cycle) Pathway Diagram

Diseases associated with MDH2 active protein

Disease Name	Pubmed Publications
Carcinoma Antibodies	>5 publications with MDH2 and Carcinoma
Cardiovascular Diseases Antibodies	>4 publications with MDH2 and Cardiovascular Diseases
Adenocarcinoma Antibodies	>2 publications with MDH2 and Adenocarcinoma
Fatty Liver Antibodies	>2 publications with MDH2 and Fatty Liver
Disease Models, Animal Antibodies	>1 publications with MDH2 and Disease Models, Animal
Poisoning Antibodies	>1 publications with MDH2 and Poisoning
Heart Diseases Antibodies	>1 publications with MDH2 and Heart Diseases
Hyperplasia Antibodies	>1 publications with MDH2 and Hyperplasia
Inflammation Antibodies	>1 publications with MDH2 and Inflammation
Cell Transformation, Neoplastic Antibodies	>1 publications with MDH2 and Cell Transformation, Neoplastic

Organs/Tissues associated with MDH2 active protein

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>9 publications with MDH2 and Muscle
Blood Antibodies	>7 publications with MDH2 and Blood
Brain Antibodies	>6 publications with MDH2 and Brain
Liver Antibodies	>5 publications with MDH2 and Liver
Heart Antibodies	>4 publications with MDH2 and Heart
Adrenal Gland Antibodies	>3 publications with MDH2 and Adrenal Gland
Eye Antibodies	>2 publications with MDH2 and Eye
Prostate Antibodies	>2 publications with MDH2 and Prostate
Lung Antibodies	>2 publications with MDH2 and Lung
Ovary Antibodies	>1 publications with MDH2 and Ovary