HEXIM1 Recombinant Protein

HEXIM1 is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This protein has been shown to form a protein-RNA complex composed of 7SK small nuclear RNA and positive transcription elongation factor b (P-TEFb), which is composed of cyclin-dependent kinase 9 (CDK9) and cyclin T1, and to inhibit the kinase activity of CDK9, thereby suppressing RNA polymerase II-dependent transcriptional elongation. Recombinant HEXIM1 protein was expressed in E Coli and purified by using conventional chromatography techniques.

Shimizu N., et al. (2005) Proc. Natl. Acad. Sci. u.S.A. 102 (24):8555-60; Li Q., et al. (2007) Nucleic Acids Res 35 (8):2503-12.

Small volumes of HEXIM1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Protein complexes are notorious difficult to study under native conditions. Determination of function by inhibition studies in vitro require high quality recombinant proteins to unravel individual contributors. For the RNA- transcription factor complex TefB -HEXIM1 complex, MyBiosource offers an ideal E coli cell expression produced human protein to study the science of nuclear location and kinase inhibition ( CDK 9 ). HEXIM is a known dsDNA and dsRNA binding positive transcription elongation factor, inducible with hexamethylene bis acetamide. Our protein is ideal for mol cell biol analysis, generation of anti HEXIM1 antibody pairs for assay development or for RNA polymerase ii inhibition studies in cells. This reagent has been cited in multiple crossrefmedlineweb science ↵ free full text ↵ as shown on our website informative figure. View this and related CDK9 cyclin T1 or mir 16 1 catalog entries to complete your studies with the #1 source of research reagents.