GCDH Recombinant Protein

Name: GCDH Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203597
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203597

Glutaryl-CoA dehydrogenase (GCDH) belongs to the acyl-CoA dehydrogenase family. GCDH catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO (2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. This… More details

$245 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GCDH, Recombinant Protein

Full Product Name

GCDH, 45-438aa, Human, His tag, E Coli

Product Gene Name

GCDH recombinant protein

Product Synonym Names

Glutaryl-CoA dehydrogenase; ACAD5; GCD; Glutaryl-CoA dehydrogenase; ACAD-5; ACAD 5

Sequence

MGSSHHHHHH SSGLVPRGSH MRPEFDWQDP LVLEEQLTTD EILIRDTFRT YCQERLMPRI LLANRNEVFH REIISEMGEL GVLGPTIKGY GCAGVSSVAY GLLARELERV DSGYRSAMSV QSSLVMHPIY AYGSEEQRQK YLPQLAKGEL LGCFGLTEPN SGSDPSSMET RAHYNSSNKS YTLNGTKTWI TNSPMADLFV VWARCEDGCI RGFLLEKGMR GLSAPRIQGK FSLRASATGM IIMDGVEVPE ENVLPGASSL GGPFGCLNNA RYGIAWGVLG ASEFCLHTAR QYALDRMQFG VPLARNQLIQ KKLADMLTEI TLGLHACLQL GRLKDQDKAA PEMVSLLKRN NCGKALDIAR QARDMLGGNG ISDEYHVIRH AMNLEAVNTY EGTHDIHALI LGRAITGIQA FTASK

Host

E Coli

Form/Format

Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 200mM NaCl

Purity/Purification

> 90% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

45.8kDa (415aa), confirmed by MALDI-TOF

OMIM

U69141 mRNA

3D Structure

ModBase 3D Structure for Q92947

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for GCDH recombinant protein

Metabolism

Applications Tested/Suitable for GCDH recombinant protein

SDS-PAGE

Product References and Citations for GCDH recombinant protein

Keyser B., et al. (2008) Hum. Mol. Genet. 17:3854-3863; Westover JB., et al. (2001) Biochemistry. 40 (46):14106-14.;

Other Notes

Small volumes of GCDH recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GCDH purchase

MBS203597 is a recombinant protein targeting GCDH. Also known as GCDH, 45-438aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for GCDH. It may not necessarily be applicable to this product.

NCBI GI #

4503943

NCBI GeneID

2639

NCBI Accession #

NP_000150

UniProt Primary Accession #

Q92947

UniProt Secondary Accession #

O14719; A8K2Z2

UniProt Related Accession #

Q92947

GenBank Nucleotide #

NM_000159.3

NCBI Official Full Name

glutaryl-CoA dehydrogenase, mitochondrial isoform a

NCBI Official Synonym Full Names

glutaryl-CoA dehydrogenase

NCBI Official Symbol

GCDH

NCBI Official Synonym Symbols

GCD; ACAD5

UniProt Protein Name

Glutaryl-CoA dehydrogenase, mitochondrial

UniProt Gene Name

GCDH

UniProt Synonym Gene Names

GCD

NCBI Protein Information

glutaryl-CoA dehydrogenase, mitochondrial

NCBI Summary for GCDH

The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Mutations in this gene result in the metabolic disorder glutaric aciduria type 1, which is also known as glutaric acidemia type I. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 12. [provided by RefSeq, Mar 2013]

UniProt Comments for GCDH

Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO2 in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive.

Research Articles on GCDH

1. We report the allele frequencies for three known Glutaric aciduria type I low excretors GCDH variants (M405V, V400M and R227P) and note that both the M405V and V400M variants are significantly more common in the population of African ancestry compared to the general population

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GCDH recombinant protein

Reference Product	PubMed Publications
EHHADH recombinant protein	>3 publications with GCDH and EHHADH
ACADS recombinant protein	>1 publications with GCDH and ACADS
ACOX1 recombinant protein	>1 publications with GCDH and ACOX1
DLST recombinant protein	>1 publications with GCDH and DLST
ECHS1 recombinant protein	>1 publications with GCDH and ECHS1
HADHA recombinant protein	>1 publications with GCDH and HADHA

Pathways associated with GCDH recombinant protein

Products by Pathway	Pathway Diagram
Fatty Acid Beta Oxidation Pathway antibodies	Fatty Acid Beta Oxidation Pathway Diagram
Fatty Acid Degradation Pathway antibodies	Fatty Acid Degradation Pathway Diagram
Fatty Acid Degradation Pathway antibodies	Fatty Acid Degradation Pathway Diagram
Histidine, Lysine, Phenylalanine, Tyrosine, Proline And Tryptophan Catabolism Pathway antibodies	Histidine, Lysine, Phenylalanine, Tyrosine, Proline And Tryptophan Catabolism Pathway Diagram
Lysine Catabolism Pathway antibodies	Lysine Catabolism Pathway Diagram
Lysine Degradation Pathway antibodies	Lysine Degradation Pathway Diagram
Lysine Degradation Pathway antibodies	Lysine Degradation Pathway Diagram
Lysine Degradation, Lysine => Saccharopine => Acetoacetyl-CoA Pathway antibodies	Lysine Degradation, Lysine => Saccharopine => Acetoacetyl-CoA Pathway Diagram
Lysine Degradation, Lysine => Saccharopine => Acetoacetyl-CoA Pathway antibodies	Lysine Degradation, Lysine => Saccharopine => Acetoacetyl-CoA Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram

Diseases associated with GCDH recombinant protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>71 publications with GCDH and Nervous System Diseases
Brain Diseases Antibodies	>66 publications with GCDH and Brain Diseases
Glutaric Acidemia I Antibodies	>48 publications with GCDH and Glutaric Acidemia I
Atrophy Antibodies	>13 publications with GCDH and Atrophy
Liver Diseases Antibodies	>6 publications with GCDH and Liver Diseases
Necrosis Antibodies	>6 publications with GCDH and Necrosis
Kidney Diseases Antibodies	>4 publications with GCDH and Kidney Diseases
Fatty Liver Antibodies	>3 publications with GCDH and Fatty Liver
Edema Antibodies	>3 publications with GCDH and Edema
Heart Diseases Antibodies	>3 publications with GCDH and Heart Diseases

Organs/Tissues associated with GCDH recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>79 publications with GCDH and Brain
Blood Antibodies	>37 publications with GCDH and Blood
Liver Antibodies	>11 publications with GCDH and Liver
Muscle Antibodies	>7 publications with GCDH and Muscle
Heart Antibodies	>6 publications with GCDH and Heart
Kidney Antibodies	>5 publications with GCDH and Kidney
Skin Antibodies	>3 publications with GCDH and Skin
Embryonic Tissue Antibodies	>3 publications with GCDH and Embryonic Tissue
Vascular Antibodies	>2 publications with GCDH and Vascular
Intestine Antibodies	>2 publications with GCDH and Intestine