CDA Active Protein

Name: CDA Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203660
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203660

CDA (Cytidine deaminase) is an enzyme that scavenges exogenous and endogenous cytidine and 2-deoxycytidine for uMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. The protein also catalyzes the… More details

$230 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CDA, Active Protein

Full Product Name

CDA, 1-146aa, Human, His tag, E Coli (Bioactivity Validated)

Product Gene Name

CDA active protein

Product Synonym Names

Cytidine deaminase; CDD; Cytidine aminohydrolase; Cytosine nucleoside deaminase; Cytidine deaminase

Sequence

MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ

Host

E Coli

Form/Format

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1 mM DTT, 2 mM EDTA, 100 mM NaCl, 40% glycerol

Purity/Purification

> 90% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

18.3 kDa (166aa), confirmed by MALDI-TOF

OMIM

L27943 mRNA

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Human

Tag Information

His-tag

Biological Activity

Specific activity is > 10,000 pmol/min/ug, and is defined as the amount of required to deaminate 1.0 pmole of cytidine per min at pH 7.5 at 25C.

Product Categories/Family for CDA active protein

Enzymes & Proteases

Applications Tested/Suitable for CDA active protein

SDS-PAGE

Product References and Citations for CDA active protein

Evrard A., et al. (2010) Ther Drug Monit. 32 (1):53-60.; Seeberg EC., et a/. (1998) Blood. 91 (11):4127-35.;

Other Notes

Small volumes of CDA active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDA purchase

Maintaining cellular pyrimidine pool for nucleic acid synthesis is paramount for cells. Cytidine deaminase CDA enzymatically cleaves deoxycytidine and cytosine nucleoside derived chemotherapy agents such as AraC. Use of this recombinant protein enzyme is common in cell biology and organism specific biosystem analysis studies. Mybiosource, together with our supplier Creative Biomart, have teamed up to provide an outstanding recombinant human CDA. Like all our recombinant proteins, we carefully detail sequence analysis from E col protein expression production, purify and characterize using His tagged antibody assay and SDS Page kDa comparisons to CDA human lysates. Our partner has all BLAST resources to check genome variants against known human cytidine deaminase conserved domain sequences to maximize protein production and quality. Besides enzymatic capabilities, this protein can be used to generate an antibody with sensitivity cytosine nucleoside to pair with commercial products in ELISA assay protein kits development. Learn _ at MyBiosource.com

Additional Details

NCBI/Uniprot data below describe general gene information for CDA. It may not necessarily be applicable to this product.

NCBI GI #

32450432

NCBI GeneID

978

NCBI Accession #

AAH54036

UniProt Related Accession #

P32320

NCBI Official Full Name

Cytidine deaminase

NCBI Official Synonym Full Names

cytidine deaminase

NCBI Official Symbol

CDA

NCBI Official Synonym Symbols

CDD

UniProt Protein Name

Cytidine deaminase

UniProt Synonym Protein Names

Cytidine aminohydrolase

Protein Family

Calcium-transporting ATPase

UniProt Gene Name

CDA

UniProt Synonym Gene Names

CDD

NCBI Protein Information

cytidine deaminase

NCBI Summary for CDA

This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias. [provided by RefSeq, Jul 2008]

UniProt Comments for CDA

This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.

Research Articles on CDA

1. results indicate that rs2072671 in CDA may be an important prognostic marker in NK-AML patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CDA active protein

Reference Product	PubMed Publications
DCK active protein	>85 publications with CDA and DCK
TK1 active protein	>4 publications with CDA and TK1
PNP active protein	>3 publications with CDA and PNP
TYMP active protein	>3 publications with CDA and TYMP
UCK2 active protein	>1 publications with CDA and UCK2

Pathways associated with CDA active protein

Products by Pathway	Pathway Diagram
Drug Metabolism - Other Enzymes Pathway antibodies	Drug Metabolism - Other Enzymes Pathway Diagram
Drug Metabolism - Other Enzymes Pathway antibodies	Drug Metabolism - Other Enzymes Pathway Diagram
Fluoropyrimidine Activity Pathway antibodies	Fluoropyrimidine Activity Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Nucleotides Pathway antibodies	Metabolism Of Nucleotides Pathway Diagram
Neutrophil Degranulation Pathway antibodies	Neutrophil Degranulation Pathway Diagram
Pyrimidine Metabolism Pathway antibodies	Pyrimidine Metabolism Pathway Diagram

Diseases associated with CDA active protein

Disease Name	Pubmed Publications
Anemia Antibodies	>283 publications with CDA and Anemia
Anemia, Hemolytic Antibodies	>224 publications with CDA and Anemia, Hemolytic
Nervous System Diseases Antibodies	>119 publications with CDA and Nervous System Diseases
Neutropenia Antibodies	>95 publications with CDA and Neutropenia
Lung Diseases Antibodies	>89 publications with CDA and Lung Diseases
Thrombocytopenia Antibodies	>74 publications with CDA and Thrombocytopenia
Carcinoma Antibodies	>67 publications with CDA and Carcinoma
Liver Diseases Antibodies	>46 publications with CDA and Liver Diseases
Neoplasms, Experimental Antibodies	>40 publications with CDA and Neoplasms, Experimental
Leukopenia Antibodies	>39 publications with CDA and Leukopenia

Organs/Tissues associated with CDA active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>634 publications with CDA and Blood
Bone Antibodies	>375 publications with CDA and Bone
Bone Marrow Antibodies	>245 publications with CDA and Bone Marrow
Brain Antibodies	>144 publications with CDA and Brain
Liver Antibodies	>100 publications with CDA and Liver
Lung Antibodies	>74 publications with CDA and Lung
Skin Antibodies	>73 publications with CDA and Skin
Kidney Antibodies	>71 publications with CDA and Kidney
Connective Tissue Antibodies	>37 publications with CDA and Connective Tissue
Eye Antibodies	>34 publications with CDA and Eye