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uDP Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203674

uridine phosphorypase (uDP) catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
uDP, Active Protein
Full Product Name
uDP, 1-253aa, E Coli, His tag, E Coli (Bioactivity Validated)
Product Gene Name
Product Synonym Names
uridine phosphorylase; urdPase; upase; uridine phosphorylase
Sequence
MGSSHHHHHH SSGLVPRGSH MSKSDVFHLG LTKNDLQGAT LAIVPGDPDR VEKIAALMDK PVKLASHREF TTWRAELDGK PVIVCSTGIG GPSTSIAVEE LAQLGIRTFL RIGTTGAIQP HINVGDVLVT TASVRLDGAS LHFAPLEFPA VADFECTTAL VEAAKSIGAT THVGVTASSD TFYPGQERYD TYSGRVVRHF KGSMEEWQAM GVMNYEMESA TLLTMCASQG LRAGMVAGVI VNRTQQEIPN AETMKQTESH AVKIVVEAAR RLL
Host
E Coli
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50mM NaCl
Purity/Purification
> 95% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
29.3kDa (273aa) confirmed by MALDI-TOF
3D Structure
ModBase 3D Structure for P12758
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Antigen Species
E Coli
Tag Information
His-tag
Biological Activity
Specific activity is > 20,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of uridine presence of phosphate per minute at pH 7.5 at 25C.

Product Categories/Family for uDP active protein

Applications Tested/Suitable for uDP active protein
SDS-PAGE

SDS-Page of uDP active protein

uDP Active Protein – Cat# MBS203674

Product References and Citations for uDP active protein

Burling F.T., et al. (2003) Acta Crystallogr. 59:73-76; Walton L., et al (1989) Nucleic Acids Res. 17 (16):6741.;

Other Notes

Small volumes of uDP active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for uDP purchase

MBS203674 is an active protein targeting uDP. Also known as uDP, 1-253aa, E Coli, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for uDP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
uridine phosphorylase
NCBI Official Symbol
udp
NCBI Official Synonym Symbols
ECK3825; JW3808
UniProt Protein Name
Uridine phosphorylase
Protein Family
UniProt Gene Name
udp
UniProt Synonym Gene Names
UPase; UrdPase
NCBI Protein Information
uridine phosphorylase

NCBI Summary for uDP

Binds TrxA (Kumar, 2004). [More information is available at EcoGene: EG11045].

UniProt Comments for uDP

Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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