3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Alpha-Enolase (ENO1) Active Protein

Name: ENO1 Active Protein; Alpha-Enolase; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203689
Price: 220 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203689

Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Alpha-enolase (ENO1), Active Protein

Full Product Name

Alpha-enolase, 1-434aa, Human, E Coli (Bioactivity Validated)

Product Gene Name

ENO1 active protein

Product Synonym Names

Non-Neuronal Enolase (NNE); Enolase-1; ENO1; MBP-1; MYC promoter-binding protein 1; PPH; tau-crystallin; 2 phospho D glycerate hydro lyase; Alpha enolase; C myc promoter binding protein; EC 4.2.1.11; ENO1L1; Enolase 1; Enolase 1 (alpha); Enolase 1 (alpha) like 1; MBP 1; MBP1; MBPB1; MPB 1; MPB1; PPH; MYC promoter binding protein 1; NNE; Non neural enolase; Phosphopyruvate hydratase; Plasminogen binding protein

Sequence

MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK

Host

E Coli

Form/Format

Liquid. In 20 mM Tris-HCl Buffer (pH 7.5) containing 1 mM MgSO4, 10% glycerol

Purity/Purification

> 95% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 2-8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Molecular Weight

47.1 kDa (434aa), confirmed by MALDI-TOF.

Sequence Positions

1-434aa

OMIM

M14328 mRNA

3D Structure

ModBase 3D Structure for P06733

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Human

Endotoxin Level

< 1 EU per 1ug of protein (determined by LAL method)

Biological Activity

Specific activity is > 20,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.

Product Categories/Family for ENO1 active protein

Metabolism

Applications Tested/Suitable for ENO1 active protein

Enzyme Activity, SDS-PAGE

Product References and Citations for ENO1 active protein

Validating Antibodies to the Cannabinoid CB2 Receptor Antibody Sensitivity Is Not Evidence of Antibody Specificity. Yannick Marchalant, Philip W. Brownjohn, Amandine Bonnet, Torsten Kleffmann, John C. Ashton (2014)

Other Notes

Small volumes of ENO1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ENO1 purchase

MBS203689 is an active protein targeting ENO1. Also known as Alpha-enolase, 1-434aa, Human, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ENO1. It may not necessarily be applicable to this product.

NCBI GI #

4503571

NCBI GeneID

2023

NCBI Accession #

NP_001419

UniProt Primary Accession #

P06733

UniProt Secondary Accession #

P22712; Q16704; Q4TUS4; Q53FT9; Q53HR3; Q658M5; Q6GMP2; Q71V37; Q7Z3V6; Q8WU71; B2RD59

UniProt Related Accession #

P06733

GenBank Nucleotide #

NM_001428.4

NCBI Official Full Name

alpha-enolase isoform 1

NCBI Official Synonym Full Names

enolase 1

NCBI Official Symbol

ENO1

NCBI Official Synonym Symbols

NNE; PPH; MPB1; ENO1L1; HEL-S-17

UniProt Protein Name

Alpha-enolase

UniProt Synonym Protein Names

2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein

Protein Family

Alpha-enolase

UniProt Gene Name

ENO1

UniProt Synonym Gene Names

ENO1L1; MBPB1; MPB1; NNE

NCBI Protein Information

alpha-enolase; c-myc promoter-binding protein-1

NCBI Summary for ENO1

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]

UniProt Comments for ENO1

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.

Research Articles on ENO1

1. ENO1 promotes in pancreatic ductal adenocarcinoma (PDA) cells cell survival, migration, and metastasis through cooperation with integrins and uPAR

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ENO1 active protein

Reference Product	PubMed Publications
PGK1 active protein	>20 publications with ENO1 and PGK1
GAPDH active protein	>16 publications with ENO1 and GAPDH
TPI1 active protein	>13 publications with ENO1 and TPI1
PGAM1 active protein	>8 publications with ENO1 and PGAM1
PKM active protein	>6 publications with ENO1 and PKM
BPGM active protein	>2 publications with ENO1 and BPGM
PGAM2 active protein	>2 publications with ENO1 and PGAM2
PKLR active protein	>2 publications with ENO1 and PKLR

Pathways associated with ENO1 active protein

Products by Pathway	Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Gluconeogenesis Pathway antibodies	Gluconeogenesis Pathway Diagram
Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies	Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies	Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
Glucose Metabolism Pathway antibodies	Glucose Metabolism Pathway Diagram
Glycolysis Pathway antibodies	Glycolysis Pathway Diagram
Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies	Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram

Diseases associated with ENO1 active protein

Disease Name	Pubmed Publications
Carcinoma Antibodies	>57 publications with ENO1 and Carcinoma
Lung Neoplasms Antibodies	>29 publications with ENO1 and Lung Neoplasms
Adenocarcinoma Antibodies	>27 publications with ENO1 and Adenocarcinoma
Lung Diseases Antibodies	>27 publications with ENO1 and Lung Diseases
Neoplasms, Experimental Antibodies	>23 publications with ENO1 and Neoplasms, Experimental
Arthritis, Rheumatoid Antibodies	>21 publications with ENO1 and Arthritis, Rheumatoid
Breast Neoplasms Antibodies	>20 publications with ENO1 and Breast Neoplasms
Inflammation Antibodies	>18 publications with ENO1 and Inflammation
Carcinoma, Non-Small-Cell Lung Antibodies	>16 publications with ENO1 and Carcinoma, Non-Small-Cell Lung
Neoplasm Invasiveness Antibodies	>12 publications with ENO1 and Neoplasm Invasiveness

Organs/Tissues associated with ENO1 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>76 publications with ENO1 and Blood
Lung Antibodies	>45 publications with ENO1 and Lung
Brain Antibodies	>39 publications with ENO1 and Brain
Liver Antibodies	>23 publications with ENO1 and Liver
Vascular Antibodies	>20 publications with ENO1 and Vascular
Muscle Antibodies	>19 publications with ENO1 and Muscle
Eye Antibodies	>15 publications with ENO1 and Eye
Kidney Antibodies	>15 publications with ENO1 and Kidney
Bone Antibodies	>13 publications with ENO1 and Bone
Nerve Antibodies	>10 publications with ENO1 and Nerve