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PCBD1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203693

Pterin-4-alpha-carbinolamine dehydratase precursor, also known as PCBD1, is a component of the phenylalanine hydroxylase (PAH) system and participates in tetrahydrobiopterin biosynthesis. A deficiency of this enzyme leads to hyperphenylalaninemia. The enzyme… More details

$230 / 0.02 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PCBD1, Recombinant Protein
Full Product Name
PCBD1, 1-104aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
Pterin-4-alpha-carbinolamine dehydratase; DCOH; PCBD; PCD; PHS
Sequence
MGSSHHHHHH SSGLVPRGSH MAGKAHRLSA EERDQLLPNL RAVGWNELEG RDAIFKQFHF KDFNRAFGFM TRVALQAEKL DHHPEWFNVY NKVHITLSTH ECAGLSERDI NLASFIEQVA VSMT
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
14.1 kDa (124aa), confirmed by MALDI-TOF
OMIM
M83742 mRNA
3D Structure
ModBase 3D Structure for P61457
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for PCBD1 recombinant protein

Applications Tested/Suitable for PCBD1 recombinant protein
SDS-PAGE

Product References and Citations for PCBD1 recombinant protein

Schallreuter K u., et al. (2004) Biochem Biophys Res Commun. 315:502-508.; Hasse S., et al. (2005) Exp Dermatol. 14:182-187.;

Other Notes

Small volumes of PCBD1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PCBD1 purchase

MBS203693 is a recombinant protein targeting PCBD1. Also known as PCBD1, 1-104aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PCBD1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
pterin-4-alpha-carbinolamine dehydratase isoform 1
NCBI Official Synonym Full Names
pterin-4 alpha-carbinolamine dehydratase 1
NCBI Official Symbol
PCBD1
NCBI Official Synonym Symbols
PCD; PHS; DCOH; PCBD
UniProt Protein Name
Pterin-4-alpha-carbinolamine dehydratase
UniProt Synonym Protein Names
4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; DCoH; Dimerization cofactor of HNF1; Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase; PCD
UniProt Gene Name
PCBD1
UniProt Synonym Gene Names
DCOH; PCBD; PHS; DCoH; Dimerization cofactor of HNF1; PCD
NCBI Protein Information
pterin-4-alpha-carbinolamine dehydratase

NCBI Summary for PCBD1

This gene encodes a member of the pterin-4-alpha-carbinolamine dehydratase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein functions as both a dehydratase involved in tetrahydrobiopterin biosynthesis, and as a cofactor for HNF1A-dependent transcription. A deficiency of this enzyme leads to hyperphenylalaninemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
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UniProt Comments for PCBD1

Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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