LGALS8 Active Protein

Name: LGALS8 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203709
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203709

LGALS8, also known as prostate-specific membrane antigen (PCTA1), is an additional prostate-specific antigen that is overexpressed only in malignant tumors and therefore is a more specific identifier of malignancies. It is a member of the galectin gene family… More details

$230 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

LGALS8, Active Protein

Full Product Name

LGALS8, 1-317aa, Human, His tag, E Coli (Bioactivity Validated)

Product Gene Name

LGALS8 active protein

Product Synonym Names

Galectin-8; Gal-8; PCTA1; Po66-CBP; Prostate carcinoma tumor antigen 1; Galectin-8

Sequence

MGSSHHHHHH SSGLVPRGSH MMLSLNNLQN IIYNPVIPFV GTIPDQLDPG TLIVIRGHVP SDADRFQVDL QNGSSMKPRA DVAFHFNPRF KRAGCIVCNT LINEKWGREE ITYDTPFKRE KSFEIVIMVL KDKFQVAVNG KHTLLYGHRI GPEKIDTLGI YGKVNIHSIG FSFSSDLQST QASSLELTEI SRENVPKSGT PQLRLPFAAR LNTPMGPGRT VVVKGEVNAN AKSFNVDLLA GKSKDIALHL NPRLNIKAFV RNSFLQESWG EEERNITSFP FSPGMYFEMI IYCDVREFKV AVNGVHSLEY KHRFKELSSI DTLEINGDIH LLEVRSW

Host

E Coli

Form/Format

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

Purity/Purification

> 90% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

37.9 kDa (337aa), confirmed by MALDI-TOF

OMIM

L78132 mRNA

3D Structure

ModBase 3D Structure for O00214

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Human

Tag Information

His-tag

Biological Activity

The ED50 for this effect is 5 - 10 ug/ml. Measured by its ability to agglutinate human red blood cells.

Assay

1. Mix equal volumes of human blood and Alsever's solution (pH 7.0).
(Alsever's solution: NaCl 0.42 g, Sodium citric acid 0.8g, Citric acid 0.055 g, D-glucose 2.05g in DW100 ml)
2. Centrifuge at 15000rpm for 10 minutes and wash four times with PBS.
3. Dilute packed cells in a 0.5 mg/ml trypsin-EDTA solution to give 4% red cell suspension.
4. Incubate for 1h at 37C and wash four times with PBS.
5. Dilute packed cells in PBS to give 4% red cell suspension.
6. Load 50ul of 0.5%BSA-in-0.15M-NaCl solution and 25ul of 4%-Red-Cell-in-PBS in u shaped wells.
7. Add 25ul of serial diluted galectin protein in PBS to each well plate. (Round bottom 96 well plate)
8. Incubate for 30min at room temperature to observe visible agglutination.

Product Categories/Family for LGALS8 active protein

Cancer

Applications Tested/Suitable for LGALS8 active protein

SDS-PAGE

Product References and Citations for LGALS8 active protein

Kobayashi T., et al. (2010) Leukemia. 24 (4):843-50.; Streetly MJ., et al. (2010) Blood. 115 (19):3939-48.;

Other Notes

Small volumes of LGALS8 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LGALS8 purchase

MBS203709 is an active protein targeting LGALS8. Also known as LGALS8, 1-317aa, Human, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for LGALS8. It may not necessarily be applicable to this product.

NCBI GI #

42544187

NCBI GeneID

3964

NCBI Accession #

NP_963837

UniProt Primary Accession #

O00214

UniProt Secondary Accession #

O15215; Q5T3P5; Q5T3Q4; Q8TEV1; Q96B92; Q9BXC8; Q9H584; Q9H585; Q9UEZ6; Q9UP32; Q9UP33

UniProt Related Accession #

O00214

GenBank Nucleotide #

NM_201543.2

NCBI Official Full Name

galectin-8 isoform b

NCBI Official Synonym Full Names

galectin 8

NCBI Official Symbol

LGALS8

NCBI Official Synonym Symbols

Gal-8; PCTA1; PCTA-1; Po66-CBP

UniProt Protein Name

Galectin-8

UniProt Synonym Protein Names

Po66 carbohydrate-binding protein; Po66-CBP; Prostate carcinoma tumor antigen 1

Protein Family

Galectin

UniProt Gene Name

LGALS8

UniProt Synonym Gene Names

PCTA-1

NCBI Protein Information

galectin-8

NCBI Summary for LGALS8

This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

UniProt Comments for LGALS8

Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy (PubMed:22246324, PubMed:28077878). Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture (PubMed:22246324, PubMed:28077878). Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52 (PubMed:22246324, PubMed:28077878). Required to restrict infection of bacterial invasion such as S.typhimurium (PubMed:22246324). Also required to restrict infection of Picornaviridae viruses (PubMed:28077878). Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans (PubMed:21288902).

Research Articles on LGALS8

1. Both Gal-8 isoforms led to enhanced adhesion of myeloma cells to vascular endothelium under dynamic shear stress conditions, Gal-8L (by more than 40-fold) even stronger than Gal-8S.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with LGALS8 active protein

Disease Name	Pubmed Publications
Carcinoma Antibodies	>21 publications with LGALS8 and Carcinoma
Inflammation Antibodies	>11 publications with LGALS8 and Inflammation
Lung Neoplasms Antibodies	>9 publications with LGALS8 and Lung Neoplasms
Neoplasm Metastasis Antibodies	>9 publications with LGALS8 and Neoplasm Metastasis
Breast Neoplasms Antibodies	>8 publications with LGALS8 and Breast Neoplasms
Carcinoma, Squamous Cell Antibodies	>7 publications with LGALS8 and Carcinoma, Squamous Cell
Prostatic Neoplasms Antibodies	>6 publications with LGALS8 and Prostatic Neoplasms
Neoplasms, Experimental Antibodies	>6 publications with LGALS8 and Neoplasms, Experimental
Kidney Diseases Antibodies	>5 publications with LGALS8 and Kidney Diseases
Cell Transformation, Neoplastic Antibodies	>5 publications with LGALS8 and Cell Transformation, Neoplastic

Organs/Tissues associated with LGALS8 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>37 publications with LGALS8 and Blood
Lung Antibodies	>11 publications with LGALS8 and Lung
Kidney Antibodies	>9 publications with LGALS8 and Kidney
Vascular Antibodies	>8 publications with LGALS8 and Vascular
Prostate Antibodies	>8 publications with LGALS8 and Prostate
Bone Antibodies	>6 publications with LGALS8 and Bone
Brain Antibodies	>6 publications with LGALS8 and Brain
Bone Marrow Antibodies	>5 publications with LGALS8 and Bone Marrow
Muscle Antibodies	>4 publications with LGALS8 and Muscle
Stomach Antibodies	>4 publications with LGALS8 and Stomach