FKBP14 Active Protein

Name: FKBP14 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203728
Price: 220 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203728

FKBP14, also known as 22 kDa FK506-binding protein, is an enzyme that accelerates the folding of proteins during protein synthesis. This protein contains two EF-hand domains and one PPIase FKBP-type domain. Truncation of the amino-terminus of FKBP14 greatly… More details

$220 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

FKBP14, Active Protein

Full Product Name

FKBP14, 20-211aa, Human, His tag, E Coli (Bioactivity Validated)

Product Gene Name

FKBP14 active protein

Product Synonym Names

Peptidyl-prolyl cis-trans isomerase FKBP14; FKBP22; Peptidyl-prolyl cis-trans isomerase FKBP14; 22 kDa FK506 binding protein; FK506 binding protein 14 (22 kDa); FK506 binding protein 14; FKBP 22; FKBP22; Peptidyl prolyl cis trans isomerase; PPIase; Rotamase

Sequence

MGSSHHHHHH SSGLVPRGSH MALIPEPEVK IEVLQKPFIC HRKTKGGDLM LVHYEGYLEK DGSLFHSTHK HNNGQPIWFT LGILEALKGW DQGLKGMCVG EKRKLIIPPA LGYGKEGKGK IPPESTLIFN IDLLEIRNGP RSHESFQEMD LNDDWKLSKD EVKAYLKKEF EKHGAVVNES HHDALVEDIF DKEDEDKDGF ISAREFTYKH DEL

Host

E Coli

Form/Format

Liquid In phosphate-buffered saline (pH 7.4) containing 10% glycerol

Purity/Purification

> 90% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

24.2 kDa (213aa) confirmed by MALDI-TOF

OMIM

AY358643 mRNA

3D Structure

ModBase 3D Structure for Q9NWM8

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Human

Tag Information

His-tag

Biological Activity

Specific activity is > 240 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.

Assay

1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
2. Add 10ul of recombinant FKBP14 protein with 1ug in assay buffer.
3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer.
4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
5. Record the increase in A405 nm for 30 minutes at 25C.

Product Categories/Family for FKBP14 active protein

Heat Shock Proteins

Applications Tested/Suitable for FKBP14 active protein

SDS-PAGE

Product References and Citations for FKBP14 active protein

Tremmel D., et al. (2007) J Mol Biol. 369 (1):55-68.; Budiman C., et al. (2009) FEBS J. 276 (15):4091-101.;

Other Notes

Small volumes of FKBP14 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FKBP14 purchase

MBS203728 is an active protein targeting FKBP14. Also known as FKBP14, 20-211aa, Human, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for FKBP14. It may not necessarily be applicable to this product.

NCBI GI #

8923659

NCBI GeneID

55033

NCBI Accession #

NP_060416

UniProt Primary Accession #

Q9NWM8

UniProt Related Accession #

Q9NWM8

GenBank Nucleotide #

NM_017946.3

NCBI Official Full Name

peptidyl-prolyl cis-trans isomerase FKBP14

NCBI Official Synonym Full Names

FK506 binding protein 14

NCBI Official Symbol

FKBP14

NCBI Official Synonym Symbols

EDSKMH; FKBP22; IPBP12; EDSKSCL2

UniProt Protein Name

Peptidyl-prolyl cis-trans isomerase FKBP14

UniProt Synonym Protein Names

22 kDa FK506-binding protein; 22 kDa FKBP; FKBP-22; FK506-binding protein 14; FKBP-14; Rotamase

Protein Family

Peptidyl-prolyl cis-trans isomerase

UniProt Gene Name

FKBP14

UniProt Synonym Gene Names

FKBP22; PPIase FKBP14; 22 kDa FKBP; FKBP-22; FKBP-14

NCBI Protein Information

peptidyl-prolyl cis-trans isomerase FKBP14

NCBI Summary for FKBP14

The protein encoded by this gene is a member of the FK506-binding protein family of peptidyl-prolyl cis-trans isomerases. The encoded protein is found in the lumen of the endoplasmic reticulum, where it is thought to accelerate protein folding. Defects in this gene are a cause of a type of Ehlers-Danlos syndrome (EDS). Both a protein-coding variant and noncoding variants are transcribed from this gene. [provided by RefSeq, Mar 2012]

UniProt Comments for FKBP14

PPIases accelerate the folding of proteins during protein synthesis.

Research Articles on FKBP14

1. Genetic studies showed that the patient harboured a homozygous mutation (c.362dupC p.Glu122Argfs*7) in the FKBP14 gene, confirming kyphoscoliotic EDS related to this gene.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with FKBP14 active protein

Products by Pathway	Pathway Diagram
IRE1alpha Activates Chaperones Pathway antibodies	IRE1alpha Activates Chaperones Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Unfolded Protein Response (UPR) Pathway antibodies	Unfolded Protein Response (UPR) Pathway Diagram
XBP1(S) Activates Chaperone Genes Pathway antibodies	XBP1(S) Activates Chaperone Genes Pathway Diagram

Diseases associated with FKBP14 active protein

Disease Name	Pubmed Publications
Abnormalities, Multiple Antibodies	>4 publications with FKBP14 and Abnormalities, Multiple
Ovarian Neoplasms Antibodies	>2 publications with FKBP14 and Ovarian Neoplasms
Heart Diseases Antibodies	>2 publications with FKBP14 and Heart Diseases
Craniofacial Abnormalities Antibodies	>1 publications with FKBP14 and Craniofacial Abnormalities
Neoplasm Invasiveness Antibodies	>1 publications with FKBP14 and Neoplasm Invasiveness
Cardiovascular Abnormalities Antibodies	>1 publications with FKBP14 and Cardiovascular Abnormalities
Heart Defects, Congenital Antibodies	>1 publications with FKBP14 and Heart Defects, Congenital

Organs/Tissues associated with FKBP14 active protein

Organ/Tissue Name	Pubmed Publications
Connective Tissue Antibodies	>5 publications with FKBP14 and Connective Tissue
Skin Antibodies	>5 publications with FKBP14 and Skin
Vascular Antibodies	>3 publications with FKBP14 and Vascular
Bone Antibodies	>1 publications with FKBP14 and Bone
Lung Antibodies	>1 publications with FKBP14 and Lung
Eye Antibodies	>1 publications with FKBP14 and Eye