3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

SAE1 Recombinant Protein

Name: SAE1 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203729
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203729

SAE1, also known as AOS1, belongs to the ubiquitin-activating E1 family of proteins and plays an important role in the first step of the uBL1 conjugation pathway. Proteins conjugated to ub are marked for progressive degradation by the 26S Proteasome. SAE1,… More details

$230 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SAE1, Recombinant Protein

Full Product Name

SAE1, 1-346aa, Human, His-T7 tag, E Coli

Product Gene Name

SAE1 recombinant protein

Product Synonym Names

SuMO1 activating enzyme subunit 1; AOS1; HSPC140; SuA1; uBLE1A; SuMO1 activating enzyme subunit 1; ubiquitin-like 1-activating enzyme E1A; AOS 1; HSPC 140; SuA 1; uBLE 1A; uBL E1A; SAE 1; ubiquitin like protein SuMO 1 activating enzyme

Sequence

MHHHHHHMAS MTGGQQMGRD LYDDDDKDRW GSMVEKEEAG GGISEEEAAQ YDRQIRLWGL EAQKRLRASR VLLVGLKGLG AEIAKNLILA GVKGLTMLDH EQVTPEDPGA QFLIRTGSVG RNRAEASLER AQNLNPMVDV KVDTEDIEKK PESFFTQFDA VCLTCCSRDV IVKVDQICHK NSIKFFTGDV FGYHGYTFAN LGEHEFVEEK TKVAKVSQGV EDGPDTKRAK LDSSETTMVK KKVVFCPVKE ALEVDWSSEK AKAALKRTTS DYFLLQVLLK FRTDKGRDPS SDTYEEDSEL LLQIRNDVLD SLGISPDLLP EDFVRYCFSE MAPVCAVVGG ILAQEIVKAL SQRDPPHNNF FFFDGMKGNG IVECLGPK

Host

E Coli

Form/Format

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Purity/Purification

> 90% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 2-8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Molecular Weight

42.2 kDa (378aa) confirmed by MALDI-TOF

OMIM

AF090385 mRNA

3D Structure

ModBase 3D Structure for Q9UBE0

Tag Information

His-T7-tag

Product Categories/Family for SAE1 recombinant protein

Ubiquitination

Applications Tested/Suitable for SAE1 recombinant protein

SDS-PAGE

Product References and Citations for SAE1 recombinant protein

Boggio R., et al. (2007) J Biol Chem. 282 (21):15376-82.; Jones MC., et al. (2006) Proc Natl Acad Sci u S A. 103 (44):16272-7.;

Other Notes

Small volumes of SAE1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SAE1 purchase

MBS203729 is a recombinant protein targeting SAE1. Also known as SAE1, 1-346aa, Human, His-T7 tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for SAE1. It may not necessarily be applicable to this product.

NCBI GI #

4885585

NCBI GeneID

10055

NCBI Accession #

NP_005491

UniProt Primary Accession #

Q9UBE0

UniProt Secondary Accession #

O95717; Q9P020; B2RDP5; B3KMQ2; F5GXX7; G3XAK6

UniProt Related Accession #

Q9UBE0

GenBank Nucleotide #

NM_005500.2

NCBI Official Full Name

SUMO-activating enzyme subunit 1 isoform a

NCBI Official Synonym Full Names

SUMO1 activating enzyme subunit 1

NCBI Official Symbol

SAE1

NCBI Official Synonym Symbols

AOS1; SUA1; UBLE1A; HSPC140

UniProt Protein Name

SUMO-activating enzyme subunit 1

UniProt Synonym Protein Names

Ubiquitin-like 1-activating enzyme E1A

Protein Family

SUMO-activating enzyme

UniProt Gene Name

SAE1

UniProt Synonym Gene Names

AOS1; SUA1; UBLE1A

NCBI Protein Information

SUMO-activating enzyme subunit 1

NCBI Summary for SAE1

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]

UniProt Comments for SAE1

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Research Articles on SAE1

1. the expression of SENP8, SAE1, PIAS1, PIAS2 and ZMIZ1 is deregulated in the majority of PTC tissues, likely contributing to the PTC phenotype.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SAE1 recombinant protein

Reference Product	PubMed Publications
SUMO1 recombinant protein	>32 publications with SAE1 and SUMO1
UBA2 recombinant protein	>25 publications with SAE1 and UBA2
SUMO2 recombinant protein	>5 publications with SAE1 and SUMO2
SUMO3 recombinant protein	>4 publications with SAE1 and SUMO3
UBE2I recombinant protein	>3 publications with SAE1 and UBE2I
RBX1 recombinant protein	>1 publications with SAE1 and RBX1

Pathways associated with SAE1 recombinant protein

Products by Pathway	Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Post-translational Protein Modification Pathway antibodies	Post-translational Protein Modification Pathway Diagram
Processing And Activation Of SUMO Pathway antibodies	Processing And Activation Of SUMO Pathway Diagram
SUMO Is Conjugated To E1 (UBA2:SAE1) Pathway antibodies	SUMO Is Conjugated To E1 (UBA2:SAE1) Pathway Diagram
SUMO Is Transferred From E1 To E2 (UBE2I, UBC9) Pathway antibodies	SUMO Is Transferred From E1 To E2 (UBE2I, UBC9) Pathway Diagram
SUMOylation Pathway antibodies	SUMOylation Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram

Diseases associated with SAE1 recombinant protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>5 publications with SAE1 and Nervous System Diseases
Neoplasms, Experimental Antibodies	>4 publications with SAE1 and Neoplasms, Experimental
Disease Models, Animal Antibodies	>3 publications with SAE1 and Disease Models, Animal
Muscular Diseases Antibodies	>2 publications with SAE1 and Muscular Diseases
Seizures Antibodies	>1 publications with SAE1 and Seizures
Brain Diseases Antibodies	>1 publications with SAE1 and Brain Diseases
Fibrosis Antibodies	>1 publications with SAE1 and Fibrosis

Organs/Tissues associated with SAE1 recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>4 publications with SAE1 and Brain
Muscle Antibodies	>3 publications with SAE1 and Muscle
Blood Antibodies	>3 publications with SAE1 and Blood
Skin Antibodies	>3 publications with SAE1 and Skin
Lung Antibodies	>2 publications with SAE1 and Lung
Thyroid Antibodies	>1 publications with SAE1 and Thyroid
Connective Tissue Antibodies	>1 publications with SAE1 and Connective Tissue
Kidney Antibodies	>1 publications with SAE1 and Kidney
Uterus Antibodies	>1 publications with SAE1 and Uterus
Embryonic Tissue Antibodies	>1 publications with SAE1 and Embryonic Tissue