NANS Recombinant Protein

Name: NANS Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203797
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203797

NANS, also known as SAS, is a 359 amino acid protein that contains one AFP (antifreeze proteins)-like domain and functions in the biosynthesis of sialic acids. Expressed ubiquitously, NANS enzymatically catalyzes the H2O-dependent formation of… More details

$230 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NANS, Recombinant Protein

Full Product Name

NANS, 1-359aa, Human, His tag, E Coli

Product Gene Name

NANS recombinant protein

Product Synonym Names

Sialic acid synthase; SAS; N acetylneuraminate synthase

Sequence

MGSSHHHHHH SSGLVPRGSH MPLELELCPG RWVGGQHPCF IIAEIGQNHQ GDLDVAKRMI RMAKECGADC AKFQKSELEF KFNRKALERP YTSKHSWGKT YGEHKRHLEF SHDQYRELQR YAEEVGIFFT ASGMDEMAVE FLHELNVPFF KVGSGDTNNF PYLEKTAKKG RPMVISSGMQ SMDTMKQVYQ IVKPLNPNFC FLQCTSAYPL QPEDVNLRVI SEYQKLFPDI PIGYSGHETG IAISVAAVAL GAKVLERHIT LDKTWKGSDH SASLEPGELA ELVRSVRLVE RALGSPTKQL LPCEMACNEK LGKSVVAKVK IPEGTILTMD MLTVKVGEPK GYPPEDIFNL VGKKVLVTVE EDDTIMEELV DNHGKKIKS

Host

E Coli

Form/Format

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

Purity/Purification

> 95% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

42.4 kDa (379aa) confirmed by MALDI-TOF

OMIM

AF257466 mRNA

3D Structure

ModBase 3D Structure for Q9NR45

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for NANS recombinant protein

Metabolism

Applications Tested/Suitable for NANS recombinant protein

SDS-PAGE

Product References and Citations for NANS recombinant protein

Milstein O., et al. (2008) J Biol Chem. 283 (49):34414-22.; Aldape MJ., et al. (2007) J Infect Dis. 195 (12):1838-45.;

Other Notes

Small volumes of NANS recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NANS purchase

MBS203797 is a recombinant protein targeting NANS. Also known as NANS, 1-359aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for NANS. It may not necessarily be applicable to this product.

NCBI GI #

12056473

NCBI GeneID

54187

NCBI Accession #

NP_061819

UniProt Primary Accession #

Q9NR45

UniProt Secondary Accession #

Q8WUV9; Q9BWS6; Q9NVD4; B2RE98

UniProt Related Accession #

Q9NR45

GenBank Nucleotide #

NM_018946.3

NCBI Official Full Name

sialic acid synthase

NCBI Official Synonym Full Names

N-acetylneuraminate synthase

NCBI Official Symbol

NANS

NCBI Official Synonym Symbols

SAS; SEMDG; SEMDCG; HEL-S-100

UniProt Protein Name

Sialic acid synthase

UniProt Synonym Protein Names

N-acetylneuraminate synthase (EC:2.5.1.56); N-acetylneuraminate-9-phosphate synthase (EC:2.5.1.57); N-acetylneuraminic acid phosphate synthase; N-acetylneuraminic acid synthase

Protein Family

Probable 9-O-acetyl-N-acetylneuraminic acid deacetylase

UniProt Gene Name

NANS

UniProt Synonym Gene Names

SAS

NCBI Protein Information

sialic acid synthase

NCBI Summary for NANS

This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq, Jul 2008]

UniProt Comments for NANS

Produces N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN). Can also use N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively.

Research Articles on NANS

1. NANS deficiency is associated with defect in brain and skeletal development.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NANS recombinant protein

Reference Product	PubMed Publications
GNE recombinant protein	>1 publications with NANS and GNE

Pathways associated with NANS recombinant protein

Products by Pathway	Pathway Diagram
Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies	Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies	Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
Asparagine N-linked Glycosylation Pathway antibodies	Asparagine N-linked Glycosylation Pathway Diagram
Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway antibodies	Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway Diagram
CMP-N-acetylneuraminate Biosynthesis I (eukaryotes) Pathway antibodies	CMP-N-acetylneuraminate Biosynthesis I (eukaryotes) Pathway Diagram
CMP-N-acetylneuraminate Biosynthesis I (eukaryotes) Pathway antibodies	CMP-N-acetylneuraminate Biosynthesis I (eukaryotes) Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Post-translational Protein Modification Pathway antibodies	Post-translational Protein Modification Pathway Diagram
Sialic Acid Metabolism Pathway antibodies	Sialic Acid Metabolism Pathway Diagram

Diseases associated with NANS recombinant protein

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>2 publications with NANS and Cardiovascular Diseases
Kidney Diseases Antibodies	>2 publications with NANS and Kidney Diseases
Heart Diseases Antibodies	>1 publications with NANS and Heart Diseases
Weight Gain Antibodies	>1 publications with NANS and Weight Gain
Hyperplasia Antibodies	>1 publications with NANS and Hyperplasia
Developmental Disabilities Antibodies	>1 publications with NANS and Developmental Disabilities
Bone Diseases, Developmental Antibodies	>1 publications with NANS and Bone Diseases, Developmental

Organs/Tissues associated with NANS recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>7 publications with NANS and Blood
Brain Antibodies	>4 publications with NANS and Brain
Kidney Antibodies	>3 publications with NANS and Kidney
Skin Antibodies	>3 publications with NANS and Skin
Bone Antibodies	>2 publications with NANS and Bone
Ovary Antibodies	>2 publications with NANS and Ovary
Eye Antibodies	>1 publications with NANS and Eye
Intestine Antibodies	>1 publications with NANS and Intestine
Prostate Antibodies	>1 publications with NANS and Prostate
Muscle Antibodies	>1 publications with NANS and Muscle