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PNP Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203884

PNP belongs to the PNP/MTAP phosphorylase family of proteins. This protein catalyzes the reversible phosphorolysis of ribonucleosides and 2'-deoxyribonucleosides with specificity for guanine, hypoxanthine and their analogs. PNP deficiency is a rare autosomal… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PNP, Active Protein
Full Product Name
PNP, 1-289aa, Human, His tag, E Coli (Bioactivity Validated)
Product Gene Name
PNP active protein
Product Synonym Names
Purine nucleoside phosphorylase; NP; Inosine phosphorylase; PRO1837; PuNP
Sequence
MGSSHHHHHH SSGLVPRGSH MENGYTYEDY KNTAEWLLSH TKHRPQVAII CGSGLGGLTD KLTQAQIFDY GEIPNFPRST VPGHAGRLVF GFLNGRACVM MQGRFHMYEG YPLWKVTFPV RVFHLLGVDT LVVTNAAGGL NPKFEVGDIM LIRDHINLPG FSGQNPLRGP NDERFGDRFP AMSDAYDRTM RQRALSTWKQ MGEQRELQEG TYVMVAGPSF ETVAECRVLQ KLGADAVGMS TVPEVIVARH CGLRVFGFSL ITNKVIMDYE SLEKANHEEV LAAGKQAAQK LEQFVSILMA SIPLPDKAS
Host
E Coli
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 2mM DTT, 10% glycerol
Purity/Purification
> 90% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
34.2 kDa (309aa) confirmed by MALDI-TOF
OMIM
X00737 mRNA
3D Structure
ModBase 3D Structure for P00491
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Antigen Species
Human
Tag Information
His-tag
Biological Activity
Specific activity is > 120 units/mg, and is defined as the amount of enzyme that phosphorolysis of 1.0 umole of inosine with inorganic phosphate per minute at pH 7.5 at 25C.

Product Categories/Family for PNP active protein

Neuroscience
Applications Tested/Suitable for PNP active protein
SDS-PAGE

SDS-Page of PNP active protein

PNP Active Protein – Cat# MBS203884

Product References and Citations for PNP active protein

Dautant A., et al. (2010) J Biol Chem. 285 (38):29502-10.; Balakrishnan K., et al. (2010) Blood. 116 (6):886-92.;

Other Notes

Small volumes of PNP active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PNP purchase

MBS203884 is an active protein targeting PNP. Also known as PNP, 1-289aa, Human, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PNP. It may not necessarily be applicable to this product.

NCBI GI #
157168362
NCBI GeneID
4860
NCBI Accession #
NP_000261
UniProt Primary Accession #
P00491
UniProt Secondary Accession #
Q15160; Q5PZ03; B2R8S5; D3DS00
UniProt Related Accession #
P00491
GenBank Nucleotide #
NM_000270.3
NCBI Official Full Name
purine nucleoside phosphorylase
NCBI Official Synonym Full Names
purine nucleoside phosphorylase
NCBI Official Symbol
PNP
NCBI Official Synonym Symbols
NP; PUNP; PRO1837
UniProt Protein Name
Purine nucleoside phosphorylase
UniProt Synonym Protein Names
Inosine phosphorylase; Inosine-guanosine phosphorylase
Protein Family
Polyribonucleotide nucleotidyltransferase
UniProt Gene Name
PNP
UniProt Synonym Gene Names
NP; PNP
NCBI Protein Information
purine nucleoside phosphorylase

NCBI Summary for PNP

This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Jul 2008]
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UniProt Comments for PNP

The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.

Research Articles on PNP

1. The PNP rs1049564 T allele is a loss-of-function variant that induces S-phase block and IFN pathway activation in lymphocytes. The S-phase block could be rescued in our in vitro experiments, suggesting the potential for personalized treatment.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PNP active protein

 Reference Product  PubMed Publications
 ADA active protein  >165 publications with PNP and ADA
 HPRT1 active protein  >50 publications with PNP and HPRT1
 APRT active protein  >16 publications with PNP and APRT
 GDA active protein  >3 publications with PNP and GDA
 XDH active protein  >2 publications with PNP and XDH
 PGM2 active protein  >2 publications with PNP and PGM2
 ADK active protein  >1 publications with PNP and ADK

Pathways associated with PNP active protein

 Products by Pathway  Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Neutrophil Degranulation Pathway antibodies  Neutrophil Degranulation Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Purine Catabolism Pathway antibodies  Purine Catabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram

Diseases associated with PNP active protein

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >99 publications with PNP and Cardiovascular Diseases
 Lung Diseases Antibodies  >65 publications with PNP and Lung Diseases
 Disease Models, Animal Antibodies  >61 publications with PNP and Disease Models, Animal
 Purine Nucleoside Phosphorylase Deficiency Antibodies  >46 publications with PNP and Purine Nucleoside Phosphorylase Deficiency
 Fibrosis Antibodies  >45 publications with PNP and Fibrosis
 Heart Diseases Antibodies  >44 publications with PNP and Heart Diseases
 Neoplasms, Experimental Antibodies  >39 publications with PNP and Neoplasms, Experimental
 Inflammation Antibodies  >35 publications with PNP and Inflammation
 Necrosis Antibodies  >31 publications with PNP and Necrosis
 Adenocarcinoma Antibodies  >25 publications with PNP and Adenocarcinoma

Organs/Tissues associated with PNP active protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >606 publications with PNP and Blood
 Liver Antibodies  >274 publications with PNP and Liver
 Brain Antibodies  >176 publications with PNP and Brain
 Heart Antibodies  >166 publications with PNP and Heart
 Skin Antibodies  >139 publications with PNP and Skin
 Vascular Antibodies  >112 publications with PNP and Vascular
 Lung Antibodies  >87 publications with PNP and Lung
 Kidney Antibodies  >79 publications with PNP and Kidney
 Bone Antibodies  >75 publications with PNP and Bone
 Spleen Antibodies  >69 publications with PNP and Spleen
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