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PNPO Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203943

PNPO, also known as pyridoxine-5'-phosphate oxidase, is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6, or pyridoxal 5-prime-phosphate (PLP), is critical for normal cellular function, and some cancer cells have notable differences in vitamin B6… More details

$230 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PNPO, Recombinant Protein
Full Product Name
PNPO, 57-261aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
Pyridoxine-5'-phosphate oxidase; Pyridoxine-5'-phosphate oxidase; FLJ10535; PDXPO
Sequence
MGSSHHHHHH SSGLVPRGSH MDPVKQFAAW FEEAVQCPDI GEANAMCLAT CTRDGKPSAR MLLLKGFGKD GFRFFTNFES RKGKELDSNP FASLVFYWEP LNRQVRVEGP VKKLPEEEAE CYFHSRPKSS QIGAVVSHQS SVIPDREYLR KKNEELEQLY QDQEVPKPKS WGGYVLYPQV MEFWQGQTNR LHDRIVFRRG LPTGDSPLGP MTHRGEEDWL YERLAP
Host
E Coli
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl, 0.1mM PMSF
Purity/Purification
> 90% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
25.9 kDa (226aa) confirmed by MALDI-TOF (molecular weight on SDS-PAGE will appear higher)
OMIM
AF468030 mRNA
3D Structure
ModBase 3D Structure for Q9NVS9
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for PNPO recombinant protein

Applications Tested/Suitable for PNPO recombinant protein
SDS-PAGE

Product References and Citations for PNPO recombinant protein

Ngo EO., et al. (1998), Biochemistry., 37 (21):7741-8; Kang JH., et al. (2004). Eur J Biochem., 271 (12):2452-61

Other Notes

Small volumes of PNPO recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PNPO purchase

MBS203943 is a recombinant protein targeting PNPO. Also known as PNPO, 57-261aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PNPO. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
pyridoxine-5'-phosphate oxidase
NCBI Official Synonym Full Names
pyridoxamine 5'-phosphate oxidase
NCBI Official Symbol
PNPO
NCBI Official Synonym Symbols
PDXPO; HEL-S-302
UniProt Protein Name
Pyridoxine-5'-phosphate oxidase
UniProt Synonym Protein Names
Pyridoxamine-phosphate oxidase
UniProt Gene Name
PNPO
NCBI Protein Information
pyridoxine-5'-phosphate oxidase

NCBI Summary for PNPO

The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq, Oct 2008]
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UniProt Comments for PNPO

Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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