PPIL4 Active Protein

Name: PPIL4 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203964
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203964

PPIL4, also known as HDCME13P, is evolutionarily conserved member of the cyclophilin-type PPIase family of proteins. ubiquitously expressed with predominant expression in kidney, PPIL4 localizes to the nucleus and contains one PPIase cyclophilin-type domain,… More details

$245 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PPIL4, Active Protein

Full Product Name

PPIL4, 1-492aa, Human, His tag, E Coli (Bioactivity Validated)

Product Gene Name

PPIL4 active protein

Product Synonym Names

Peptidyl-prolyl cis-trans isomerase-like 4; HDCME13P; Rotamase PPIL4; Cyclophilin-like protein PPIL4

Sequence

MGSSHHHHHH SSGLVPRGSH MAVLLETTLG DVVIDLYTEE RPRACLNFLK LCKIKYYNYC LIHNVQRDFI IQTGDPTGTG RGGESIFGQL YGDQASFFEA EKVPRIKHKK KGTVSMVNNG SDQHGSQFLI TTGENLDYLD GVHTVFGEVT EGMDIIKKIN ETFVDKDFVP YQDIRINHTV ILDDPFDDPP DLLIPDRSPE PTREQLDSGR IGADEEIDDF KGRSAEEVEE IKAEKEAKTQ AILLEMVGDL PDADIKPPEN VLFVCKLNPV TTDEDLEIIF SRFGPIRSCE VIRDWKTGES LCYAFIEFEK EEDCEKAFFK MDNVLIDDRR IHVDFSQSVA KVKWKGKGGK YTKSDFKEYE KEQDKPPNLV LKDKVKPKQD TKYDLILDEQ AEDSKSSHSH TSKKHKKKTH HCSEEKEDED YMPIKNTNQD IYREMGFGHY EEEESCWEKQ KSEKRDRTQN RSRSRSRERD GHYSNSHKSK YQTDLYERER SKKRDRSRSP KKSKDKEKSK YR

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Purity/Purification

> 85% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

59.4 kDa (512aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

OMIM

AF357880 mRNA

3D Structure

ModBase 3D Structure for Q8WUA2

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Human

Tag Information

His-tag

Biological Activity

Specific activity is > 190 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin

Assay

1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
2. Add 10ul of recombinant PPIL4 protein with 1ug in assay buffer.
3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer.
4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
5. Record the increase in A405 nm for 30 minutes at 25C.

Product Categories/Family for PPIL4 active protein

Heat Shock Proteins

Applications Tested/Suitable for PPIL4 active protein

SDS-PAGE

Product References and Citations for PPIL4 active protein

Nebert DW., et al. (2004) Hum Genomics. 1 (5):381-8. ; Valle C., et al. (2005) Parasitol Res. 96 (4):199-205.;

Other Notes

Small volumes of PPIL4 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPIL4 purchase

MBS203964 is an active protein targeting PPIL4. Also known as PPIL4, 1-492aa, Human, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PPIL4. It may not necessarily be applicable to this product.

NCBI GI #

20911035

NCBI GeneID

85313

NCBI Accession #

NP_624311

UniProt Primary Accession #

Q8WUA2

UniProt Secondary Accession #

Q7Z3Q5; B2RD34

UniProt Related Accession #

Q8WUA2

GenBank Nucleotide #

NM_139126.3

NCBI Official Full Name

peptidyl-prolyl cis-trans isomerase-like 4

NCBI Official Synonym Full Names

peptidylprolyl isomerase like 4

NCBI Official Symbol

PPIL4

NCBI Official Synonym Symbols

HDCME13P

UniProt Protein Name

Peptidyl-prolyl cis-trans isomerase-like 4

UniProt Synonym Protein Names

Cyclophilin-like protein PPIL4; Rotamase PPIL4

Protein Family

Peptidyl-prolyl cis-trans isomerase

UniProt Gene Name

PPIL4

UniProt Synonym Gene Names

PPIase

NCBI Protein Information

peptidyl-prolyl cis-trans isomerase-like 4

NCBI Summary for PPIL4

This gene is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. [provided by RefSeq, Jul 2008]

UniProt Comments for PPIL4

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides ().

Research Articles on PPIL4

1. Molecular cloning, structure and expression of a novel nuclear RNA-binding cyclophilin-like gene (PPIL4) from human fetal brain

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with PPIL4 active protein

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>1 publications with PPIL4 and Kidney
Brain Antibodies	>1 publications with PPIL4 and Brain