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uROS Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204055

uROS, also known as uroporphyrinogen III synthase, is an enzyme involved in the fourth step of porphyrin metabolism, involved in the conversion of hydroxymethyl bilane into uroporphyrinogen III. Defects in this protein can cause molecular lesions that lead to… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
uROS, Recombinant Protein
Full Product Name
uROS, 1-265aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
uroporphyrinogen-III synthase; uroporphyrinogen III synthase
Sequence
MGSSHHHHHH SSGLVPRGSH MKVLLLKDAK EDDCGQDPYI RELGLYGLEA TLIPVLSFEF LSLPSFSEKL SHPEDYGGLI FTSPRAVEAA ELCLEQNNKT EVWERSLKEK WNAKSVYVVG NATASLVSKI GLDTEGETCG NAEKLAEYIC SRESSALPLL FPCGNLKREI LPKALKDKGI AMESITVYQT VAHPGIQGNL NSYYSQQGVP ASITFFSPSG LTYSLKHIQE LSGDNIDQIK FAAIGPTTAR ALAAQGLPVS CTAESPTPQA LATGIRKALQ PHGCC
Host
E Coli
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl
Purity/Purification
> 95% by SDS-PAGE
Concentration
1.0 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
30.7 kDa (285aa) confirmed by MALDI-TOF
OMIM
J03824 mRNA
3D Structure
ModBase 3D Structure for P10746
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for uROS recombinant protein

Applications Tested/Suitable for uROS recombinant protein
SDS-PAGE

Product References and Citations for uROS recombinant protein

Geronimi F. et al. (2003) J. Mol. Med. 81: 310-320.; Omata Y. et al. (2004) J. BioChem. 136: 211-220.

Other Notes

Small volumes of uROS recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for uROS purchase

MBS204055 is a recombinant protein targeting uROS. Also known as uROS, 1-265aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for uROS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
uroporphyrinogen-III synthase isoform 2
NCBI Official Synonym Full Names
uroporphyrinogen III synthase
NCBI Official Symbol
UROS
NCBI Official Synonym Symbols
UROIIIS
UniProt Protein Name
Uroporphyrinogen-III synthase
UniProt Synonym Protein Names
Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase
UniProt Gene Name
UROS
UniProt Synonym Gene Names
UROIIIS; UROS
NCBI Protein Information
uroporphyrinogen-III synthase

NCBI Summary for uROS

The protein encoded by this gene catalyzes the fourth step of porphyrin biosynthesis in the heme biosynthetic pathway. Defects in this gene cause congenital erythropoietic porphyria (Gunther's disease). [provided by RefSeq, Jul 2008]

UniProt Comments for uROS

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III, the branch point for the various sub-pathways leading to the wide diversity of porphyrins. Porphyrins act as cofactors for a multitude of enzymes that perform a variety of processes within the cell such as methionine synthesis (vitamin B12) or oxygen transport (heme).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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