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CLC Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204093

Eosinophil lysophospholipase (CLC), also known as Galectin 10, act on biological membranes to regulate the multifunctional lysophospholipids. CLC is a lysophospholipase expressed in eosinophils and basophils. It hydrolyzes lysophosphatidylcholine to… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CLC, Recombinant Protein
Full Product Name
CLC, 1-142aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
Eosinophil lysophospholipase; Galectin 10; Gal-10; GAL10; LGALS10; LGALS10A; LPPL
Sequence
MGSSHHHHHH SSGLVPRGSH MSLLPVPYTE AASLSTGSTV TIKGRPLACF LNEPYLQVDF HTEMKEESDI VFHFQVCFGR RVVMNSREYG AWKQQVESKN MPFQDGQEFE LSISVLPDKY QVMVNGQSSY TFDHRIKPEA VKMVQVWRDI SLTKFNVSYL KR
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Purity/Purification
> 90% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 2-8°C short term (1 week).
For long term storage, aliquot and store at -20°C or -70°C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
18.6 kDa (162aa) confirmed by MALDI-TOF
Sequence Positions
1-142aa
OMIM
L01664 mRNA
3D Structure
ModBase 3D Structure for Q05315
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for CLC recombinant protein

Applications Tested/Suitable for CLC recombinant protein
SDS-PAGE

SDS-Page of CLC recombinant protein

3 ug by SDS-PAGE under reducing condition and visualized by coomasie blue stain
CLC Recombinant Protein – Cat# MBS204093

Product References and Citations for CLC recombinant protein

Gieich G J., et al. (1976) J Clin Invest. 57:633-640.; Swaminathan G J., et al. (1999) Biochemistry 38:13837-13843.

Other Notes

Small volumes of CLC recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CLC purchase

MBS204093 is a recombinant protein targeting CLC. Also known as CLC, 1-142aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CLC. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
galectin-10
NCBI Official Synonym Full Names
Charcot-Leyden crystal galectin
NCBI Official Symbol
CLC
NCBI Official Synonym Symbols
GAL10; Gal-10; LGALS10; LGALS10A; LPPL_HUMAN
UniProt Protein Name
Galectin-10
UniProt Synonym Protein Names
Charcot-Leyden crystal protein; CLC; Eosinophil lysophospholipase; Lysolecithin acylhydrolase
Protein Family
UniProt Gene Name
CLC
UniProt Synonym Gene Names
LGALS10; LGALS10A; Gal-10; CLC
NCBI Protein Information
galectin-10

NCBI Summary for CLC

Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene is a lysophospholipase expressed in eosinophils and basophils. It hydrolyzes lysophosphatidylcholine to glycerophosphocholine and a free fatty acid. This protein may possess carbohydrate or IgE-binding activities. It is both structurally and functionally related to the galectin family of beta-galactoside binding proteins. It may be associated with inflammation and some myeloid leukemias. [provided by RefSeq, Jul 2008]
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UniProt Comments for CLC

Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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