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ASF1B Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204104

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 protein in yeast. This protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ASF1B, Recombinant Protein
Full Product Name
ASF1B, 1-202aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
ASF1 anti-silencing function 1 homolog B (S.cerevisiae); CIA-II
Sequence
MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES EEFDQILDSV LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT YHGQEFIRVG YYVNNEYLNP ELRENPPMKP DFSQLQRNIL ASNPRVTRFH INWDNNMDRL EAIETQDPSL GCGLPLNCTP IKGLGLPGCI PGLLPENSMD CILEHHHHHH
Host
E Coli
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 0.15M NaCl, 1mM DTT
Purity/Purification
> 85% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
23.4kDa (210aa), confirmed by MALDI-TOF
OMIM
AF279307 mRNA
3D Structure
ModBase 3D Structure for Q9NVP2
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for ASF1B recombinant protein

Applications Tested/Suitable for ASF1B recombinant protein
SDS-PAGE

Product References and Citations for ASF1B recombinant protein

Corpet,A., et al. (2011) EMBO J. 30 (3), 480-493; Jasencakova,Z., et al. (2010) Mol. Cell 37 (5), 736-743

Other Notes

Small volumes of ASF1B recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ASF1B purchase

MBS204104 is a recombinant protein targeting ASF1B. Also known as ASF1B, 1-202aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ASF1B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone chaperone ASF1B
NCBI Official Synonym Full Names
anti-silencing function 1B histone chaperone
NCBI Official Symbol
ASF1B
NCBI Official Synonym Symbols
CIA-II
UniProt Protein Name
Histone chaperone ASF1B
UniProt Synonym Protein Names
Anti-silencing function protein 1 homolog B; hAsf1; hAsf1b; CCG1-interacting factor A-II; CIA-II; hCIA-II
Protein Family
UniProt Gene Name
ASF1B
UniProt Synonym Gene Names
hAsf1; hAsf1b; CIA-II; hCIA-II
NCBI Protein Information
histone chaperone ASF1B

NCBI Summary for ASF1B

This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. [provided by RefSeq, Jul 2008]
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UniProt Comments for ASF1B

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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