• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

POLR2J Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204121

POLR2J is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This protein exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar… More details

$230 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POLR2J, Recombinant Protein
Full Product Name
POLR2J,1-117aa, Human, His tag, E Coli
Product Synonym Names
DNA-directed RNA polymerase II subunit RPB11-a; hRPB14; POLR2J1; RPB11; RPB11A; RPB11m
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK KITINKDTKV PNACLFTINK EDHTLGNIIK SQLLKDPQVL FAGYKVPHPL EHKIIIRVQT TPDYSPQEAF TNAITDLISE LSLLEERFRV AIKDKQEGIE
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 5mM DTT, and 2mM EDTA
Purity/Purification
> 90% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
15.7 kDa (140aa) confirmed by MALDI-TOF
OMIM
X82385 mRNA
3D Structure
ModBase 3D Structure for P52435
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for POLR2J recombinant protein

Applications Tested/Suitable for POLR2J recombinant protein
SDS-PAGE

Product References and Citations for POLR2J recombinant protein

Proshkin,S.A., et al. (2011) Biochemistry Mosc. 76 (8), 976-980; Shpakovskii,D.G., et al. (2004) Bioorg. Khim. 30 (6), 621-625

Other Notes

Small volumes of POLR2J recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLR2J purchase

MBS204121 is a recombinant protein targeting POLR2J. Also known as POLR2J,1-117aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for POLR2J. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA-directed RNA polymerase II subunit RPB11-a
NCBI Official Synonym Full Names
RNA polymerase II subunit J
NCBI Official Symbol
POLR2J
NCBI Official Synonym Symbols
RPB11; RPB11A; RPB11m; hRPB14; POLR2J1
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB11-a
UniProt Synonym Protein Names
DNA-directed RNA polymerase II subunit J-1; RNA polymerase II 13.3 kDa subunit
UniProt Gene Name
POLR2J
UniProt Synonym Gene Names
POLR2J1; RNA polymerase II subunit B11-a; RPB11a
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB11-a

NCBI Summary for POLR2J

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for POLR2J

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft ().
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with POLR2J recombinant protein

Organs/Tissues associated with POLR2J recombinant protein

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000