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DCAF7 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204129

DCAF7, also known as DDB1-and CuL4-associated factor, involved in craniofacial development. This protein acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DCAF7, Recombinant Protein
Full Product Name
DCAF7, 19-295aa, Human, His tag, E Coli (Denatured)
Product Gene Name
DCAF7 recombinant protein
Product Synonym Names
DDB1-and CuL4-associated factor 7; AN11; HAN11; SWAN-1; WDR68
Sequence
MGSSHHHHHH SSGLVPRGSH MVYAMNWSVR PDKRFRLALG SFVEEYNNKV QLVGLDEESS EFICRNTFDH PYPTTKLMWI PDTKGVYPDL LATSGDYLRV WRVGETETRL ECLLNNNKNS DFCAPLTSFD WNEVDPYLLG TSSIDTTCTI WGLETGQVLG RVNLVSGHVK TQLIAHDKEV YDIAFSRAGG GRDMFASVGA DGSVRMFDLR HLEHSTIIYE DPQHHPLLRL CWNKQDPNYL ATMAMDGMEV VILDVRVPCT PVARLNNHRA CVNGIAWAPH SSCHICTAAD DHQALIWD
Host
E Coli
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 1mM DTT
Purity/Purification
> 90% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
33.6 kDa (298aa)
OMIM
U94747 mRNA
3D Structure
ModBase 3D Structure for P61962
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for DCAF7 recombinant protein

Development
Applications Tested/Suitable for DCAF7 recombinant protein
SDS-PAGE
Application Notes for DCAF7 recombinant protein
This protein is denatured. Denatured protein is most likely not the best option for functional studies. Denatured protein is better suited for Western Blot (WB) or imaging assays. Applications are user determined.

SDS-Page of DCAF7 recombinant protein

DCAF7 Recombinant Protein – Cat# MBS204129

Product References and Citations for DCAF7 recombinant protein

Morita K., et al. (2006) J. Dermatol. Sci. 44:11-20; Jin J., et al. (2006) Mol. Cell. 23:709-721

Other Notes

Small volumes of DCAF7 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DCAF7 purchase

MBS204129 is a recombinant protein targeting DCAF7. Also known as DCAF7, 19-295aa, Human, His tag, E Coli (Denatured). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for DCAF7. It may not necessarily be applicable to this product.

NCBI GI #
108936958
NCBI GeneID
10238
NCBI Accession #
NP_005819
UniProt Primary Accession #
P61962
UniProt Secondary Accession #
O15491; Q9DAE4; B4E039; D3DU14
UniProt Related Accession #
P61962
GenBank Nucleotide #
NM_005828.4
NCBI Official Full Name
DDB1- and CUL4-associated factor 7
NCBI Official Synonym Full Names
DDB1 and CUL4 associated factor 7
NCBI Official Symbol
DCAF7
NCBI Official Synonym Symbols
AN11; HAN11; WDR68; SWAN-1
UniProt Protein Name
DDB1- and CUL4-associated factor 7
UniProt Synonym Protein Names
WD repeat-containing protein 68; WD repeat-containing protein An11 homolog
Protein Family
DDB1- and CUL4-associated factor
UniProt Gene Name
DCAF7
UniProt Synonym Gene Names
HAN11; WDR68
NCBI Protein Information
DDB1- and CUL4-associated factor 7

NCBI Summary for DCAF7

This gene encodes a protein with multiple WD40 repeats which facilitate protein-protein interactions and thereby enable the assembly of multiprotein complexes. This protein has been shown to function as a scaffold protein for protein complexes involved in kinase signaling. This highly conserved gene is present in eukaryotic plants, fungi, and animals. The ortholog of this gene was first identified in plants as a key regulator of anthocyanin biosynthesis and flower pigmentation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
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UniProt Comments for DCAF7

Involved in craniofacial development. Acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches (). Associates with DIAPH1 and controls GLI1 transcriptional activity. Could be involved in normal and disease skin development. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
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Research Articles on DCAF7

1. knockdown of DCAF7 reduced the degradation of DNA ligase I in response to inhibition of proliferation and replacement of ubiquitylated lysine residues reduced the in vitro ubiquitylation of DNA ligase I by Cul4-DDB1 and DCAF7. In contrast, a different E3 ubiquitin ligase regulates FEN-1 turnover.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DCAF7 recombinant protein

 Reference Product  PubMed Publications
 DYRK1A recombinant protein  >11 publications with DCAF7 and DYRK1A
 DYRK1B recombinant protein  >5 publications with DCAF7 and DYRK1B
 TCP1 recombinant protein  >1 publications with DCAF7 and TCP1

Pathways associated with DCAF7 recombinant protein

 Products by Pathway  Pathway Diagram
 Association Of TriC/CCT With Target Proteins During Biosynthesis Pathway antibodies  Association Of TriC/CCT With Target Proteins During Biosynthesis Pathway Diagram
 Chaperonin-mediated Protein Folding Pathway antibodies  Chaperonin-mediated Protein Folding Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Protein Folding Pathway antibodies  Protein Folding Pathway Diagram

Diseases associated with DCAF7 recombinant protein

 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >2 publications with DCAF7 and Cell Transformation, Neoplastic
 Craniofacial Abnormalities Antibodies  >2 publications with DCAF7 and Craniofacial Abnormalities
 Skin Neoplasms Antibodies  >1 publications with DCAF7 and Skin Neoplasms
 Cardiovascular Diseases Antibodies  >1 publications with DCAF7 and Cardiovascular Diseases
 Inflammation Antibodies  >1 publications with DCAF7 and Inflammation

Organs/Tissues associated with DCAF7 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >3 publications with DCAF7 and Bone
 Skin Antibodies  >3 publications with DCAF7 and Skin
 Bone Marrow Antibodies  >1 publications with DCAF7 and Bone Marrow
 Heart Antibodies  >1 publications with DCAF7 and Heart
 Blood Antibodies  >1 publications with DCAF7 and Blood
 Embryonic Tissue Antibodies  >1 publications with DCAF7 and Embryonic Tissue
 Brain Antibodies  >1 publications with DCAF7 and Brain
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