MBD3 Recombinant Protein

Name: MBD3 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS204158
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204158

Methyl-CpG-binding domain protein 3, also known as MBD3, is a member of the MBD family of transcriptional repressors. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG… More details

$245 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MBD3, Recombinant Protein

Full Product Name

MBD3, 1-291aa, Human, His tag, E Coli

Product Gene Name

MBD3 recombinant protein

Product Synonym Names

Methyl-CpG-binding domain protein 3; Methyl CpG binding domain protein 3; Methyl-CpG-binding protein MBD3

Sequence

MGSSHHHHHH SSGLVPRGSH MGSMERKRWE CPALPQGWER EEVPRRSGLS AGHRDVFYYS PSGKKFRSKP QLARYLGGSM DLSTFDFRTG KMLMSKMNKS RQRVRYDSSN QVKGKPDLNT ALPVRQTASI FKQPVTKITN HPSNKVKSDP QKAVDQPRQL FWEKKLSGLN AFDIAEELVK TMDLPKGLQG VGPGCTDETL LSAIASALHT STMPITGQLS AAVEKNPGVW LNTTQPLCKA FMVTDEDIRK QEELVQQVRK RLEEALMADM LAHVEELARD GEAPLDKACA EDDDEEDEEE EEEEPDPDPE MEHV

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 1mM DTT, 1mM EDTA

Purity/Purification

> 85% by SDS-PAGE

Concentration

0.25 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

35.2 kDa (314aa) confirmed by MALDI-TOF

OMIM

AF072247 mRNA

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for MBD3 recombinant protein

Epigenomics

Applications Tested/Suitable for MBD3 recombinant protein

SDS-PAGE

Product References and Citations for MBD3 recombinant protein

Nan X., et al. (1998) Nature. 393:386-389; Hendrich B., et al. (1999) Mamm Genome. 10: 906-912.

Other Notes

Small volumes of MBD3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MBD3 purchase

MBS204158 is a recombinant protein targeting MBD3. Also known as MBD3, 1-291aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for MBD3. It may not necessarily be applicable to this product.

NCBI GI #

4505119

NCBI GeneID

53615

NCBI Accession #

NP_003917

UniProt Secondary Accession #

Q6PIL9; Q6PJZ9; Q86XF4; A8K4B7; D6W5Z2

UniProt Related Accession #

O95983

GenBank Nucleotide #

NM_003926.5

NCBI Official Synonym Full Names

methyl-CpG binding domain protein 3

NCBI Official Symbol

MBD3

UniProt Protein Name

Methyl-CpG-binding domain protein 3

UniProt Synonym Protein Names

Methyl-CpG-binding protein MBD3

Protein Family

Methyl-CpG-binding domain-containing protein

UniProt Gene Name

MBD3

NCBI Protein Information

methyl-CpG-binding domain protein 3

NCBI Summary for MBD3

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

UniProt Comments for MBD3

Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.

Research Articles on MBD3

1. MBD3 inhibits epithelial-mesenchymal transition in pancreatic cancer cells probably via TGF-beta/Smad signaling and may be a candidate for diagnostics and prognosis of pancreatic cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MBD3 recombinant protein

Reference Product	PubMed Publications
HDAC1 recombinant protein	>21 publications with MBD3 and HDAC1
CHD4 recombinant protein	>12 publications with MBD3 and CHD4
HDAC2 recombinant protein	>11 publications with MBD3 and HDAC2
MTA1 recombinant protein	>8 publications with MBD3 and MTA1
MTA2 recombinant protein	>8 publications with MBD3 and MTA2
MTA3 recombinant protein	>4 publications with MBD3 and MTA3
GATAD2B recombinant protein	>3 publications with MBD3 and GATAD2B
RBBP7 recombinant protein	>3 publications with MBD3 and RBBP7
GATAD2A recombinant protein	>2 publications with MBD3 and GATAD2A
RBBP4 recombinant protein	>2 publications with MBD3 and RBBP4

Pathways associated with MBD3 recombinant protein

Products by Pathway	Pathway Diagram
Chromatin Modifying Enzymes Pathway antibodies	Chromatin Modifying Enzymes Pathway Diagram
Chromatin Organization Pathway antibodies	Chromatin Organization Pathway Diagram
Cytosine Methylation Pathway antibodies	Cytosine Methylation Pathway Diagram
ERCC6 (CSB) And EHMT2 (G9a) Positively Regulate RRNA Expression Pathway antibodies	ERCC6 (CSB) And EHMT2 (G9a) Positively Regulate RRNA Expression Pathway Diagram
Epigenetic Regulation Of Gene Expression Pathway antibodies	Epigenetic Regulation Of Gene Expression Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Generic Transcription Pathway antibodies	Generic Transcription Pathway Diagram
HDACs Deacetylate Histones Pathway antibodies	HDACs Deacetylate Histones Pathway Diagram
Positive Epigenetic Regulation Of RRNA Expression Pathway antibodies	Positive Epigenetic Regulation Of RRNA Expression Pathway Diagram
RNA Polymerase I Promoter Clearance Pathway antibodies	RNA Polymerase I Promoter Clearance Pathway Diagram

Diseases associated with MBD3 recombinant protein

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>11 publications with MBD3 and Disease Models, Animal
Nervous System Diseases Antibodies	>9 publications with MBD3 and Nervous System Diseases
Lung Neoplasms Antibodies	>5 publications with MBD3 and Lung Neoplasms
Adenocarcinoma Antibodies	>4 publications with MBD3 and Adenocarcinoma
Breast Neoplasms Antibodies	>4 publications with MBD3 and Breast Neoplasms
Inflammation Antibodies	>3 publications with MBD3 and Inflammation
Liver Diseases Antibodies	>3 publications with MBD3 and Liver Diseases
Learning Disorders Antibodies	>2 publications with MBD3 and Learning Disorders
Liver Neoplasms Antibodies	>2 publications with MBD3 and Liver Neoplasms
Carcinoma, Hepatocellular Antibodies	>2 publications with MBD3 and Carcinoma, Hepatocellular

Organs/Tissues associated with MBD3 recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>20 publications with MBD3 and Brain
Blood Antibodies	>14 publications with MBD3 and Blood
Skin Antibodies	>12 publications with MBD3 and Skin
Liver Antibodies	>10 publications with MBD3 and Liver
Lung Antibodies	>9 publications with MBD3 and Lung
Embryonic Tissue Antibodies	>8 publications with MBD3 and Embryonic Tissue
Nerve Antibodies	>6 publications with MBD3 and Nerve
Eye Antibodies	>6 publications with MBD3 and Eye
Testis Antibodies	>4 publications with MBD3 and Testis
Connective Tissue Antibodies	>3 publications with MBD3 and Connective Tissue