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TPM3 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204200

Tropomyosin alpha-3 chain, also known as TPM3, is member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil… More details

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Product Overview

Online Datasheet in PDF
Product Name
TPM3, Recombinant Protein
Full Product Name
TPM3, 1-248aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
Tropomyosin alpha-3 chain; hscp30; NEM1; OK/SW-cl.5; TM-5; TM3; TM30 TM30nm; TPMsk; TRK
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAGITT IEAVKRKIQV LQQQADDAEE RAERLQREVE GERRAREQAE AEVASLNRRI QLVEEELDRA QERLATALQK LEEAEKAADE SERGMKVIEN RALKDEEKME LQEIQLKEAK HIAEEADRKY EEVARKLVII EGDLERTEER AELAESRCRE MDEQIRLMDQ NLKCLSAAEE KYSQKEDKYE EEIKILTDKL KEAETRAEFA ERSVAKLEKT IDDLEDKLKC TKEEHLCTQR MLDQTLLDLN EM
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Purity/Purification
> 90% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
31.6 kDa (272aa), confirmed by MALDI-TOF
OMIM
X04201 mRNA
3D Structure
ModBase 3D Structure for P06753
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for TPM3 recombinant protein

Applications Tested/Suitable for TPM3 recombinant protein
SDS-PAGE

Product References and Citations for TPM3 recombinant protein

Ohtsuki I., et al. (2002) J Biochem. 131:739-743.; Tiso N., et al. (1997) Biochem Biophys Res Commun. 230:347-350.

Other Notes

Small volumes of TPM3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPM3 purchase

MBS204200 is a recombinant protein targeting TPM3. Also known as TPM3, 1-248aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for TPM3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tropomyosin alpha-3 chain isoform Tpm3.1cy
NCBI Official Synonym Full Names
tropomyosin 3
NCBI Official Symbol
TPM3
NCBI Official Synonym Symbols
TM3; TM5; TRK; CFTD; NEM1; TM-5; TM30; CAPM1; TM30nm; TPMsk3; hscp30; HEL-189; HEL-S-82p; OK/SW-cl.5
UniProt Protein Name
Tropomyosin alpha-3 chain
UniProt Synonym Protein Names
Gamma-tropomyosin; Tropomyosin-3; Tropomyosin-5; hTM5
Protein Family
UniProt Gene Name
TPM3
UniProt Synonym Gene Names
hTM5
NCBI Protein Information
tropomyosin alpha-3 chain

NCBI Summary for TPM3

This gene encodes a member of the tropomyosin family of actin-binding proteins. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Mutations in this gene result in autosomal dominant nemaline myopathy and other muscle disorders. This locus is involved in translocations with other loci, including anaplastic lymphoma receptor tyrosine kinase (ALK) and neurotrophic tyrosine kinase receptor type 1 (NTRK1), which result in the formation of fusion proteins that act as oncogenes. There are numerous pseudogenes for this gene on different chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
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UniProt Comments for TPM3

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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