TFB1M Recombinant Protein

Name: TFB1M Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS204218
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204218

TFB1M belongs to the methyltransferase superfamily. TFB1M is a dimethyltransferase that methylates mitochondrial 12S rRNA at the conserved stem loop. This protein also is part of the basal mitochondrial transcription complex and is necessary for mitochondrial… More details

$245 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TFB1M, Recombinant Protein

Full Product Name

TFB1M, 28-346aa, Human, His tag, E Coli

Product Gene Name

TFB1M recombinant protein

Product Synonym Names

Dimethyladenosine transferase 1; TFB1M (Mitochondrial transcription factor B1)

Sequence

MGSSHHHHHH SSGLVPRGSH MQAAKQLSQN FLLDLRLTDK IVRKAGNLTN AYVYEVGPGP GGITRSILNA DVAELLVVEK DTRFIPGLQM LSDAAPGKLR IVHGDVLTFK VEKAFSESLK RPWEDDPPNV HIIGNLPFSV STPLIIKWLE NISCRDGPFV YGRTQMTLTF QKEVAERLAA NTGSKQRSRL SVMAQYLCNV RHIFTIPGQA FVPKPEVDVG VVHFTPLIQP KIEQPFKLVE KVVQNVFQFR RKYCHRGLRM LFPEAQRLES TGRLLELADI DPTLRPRQLS ISHFKSLCDV YRKMCDEDPQ LFAYNFREEL KRRKSKNEEK EEDDAENYRL

Host

E Coli

Form/Format

Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Purity/Purification

> 90% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

38.8 kDa (340aa), confirmed by MALDI-TOF

OMIM

AF151833 mRNA

3D Structure

ModBase 3D Structure for Q8WVM0

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for TFB1M recombinant protein

Transcription & Translation

Applications Tested/Suitable for TFB1M recombinant protein

SDS-PAGE

Product References and Citations for TFB1M recombinant protein

McCulloch V., et al. (2002) Mol. Cell. Biol. 22:1116-1125; Gleyzer N., et al. (2005) Mol. Cell. Biol. 25 (4):1354-66

Other Notes

Small volumes of TFB1M recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TFB1M purchase

MBS204218 is a recombinant protein targeting TFB1M. Also known as TFB1M, 28-346aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for TFB1M. It may not necessarily be applicable to this product.

NCBI GI #

156415992

NCBI GeneID

51106

NCBI Accession #

NP_057104

UniProt Primary Accession #

Q8WVM0

UniProt Secondary Accession #

Q05DR0; Q9Y384

UniProt Related Accession #

Q8WVM0

GenBank Nucleotide #

NM_016020.3

NCBI Official Full Name

dimethyladenosine transferase 1, mitochondrial isoform 1

NCBI Official Synonym Full Names

transcription factor B1, mitochondrial

NCBI Official Symbol

TFB1M

NCBI Official Synonym Symbols

CGI75; mtTFB; CGI-75; mtTFB1

UniProt Protein Name

Dimethyladenosine transferase 1, mitochondrial

UniProt Synonym Protein Names

Mitochondrial 12S rRNA dimethylase 1; Mitochondrial transcription factor B1; h-mtTFB; h-mtTFB1; hTFB1M; mtTFB1; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1

Protein Family

Dimethyladenosine transferase

UniProt Gene Name

TFB1M

UniProt Synonym Gene Names

h-mtTFB; h-mtTFB1; hTFB1M; mtTFB1

NCBI Protein Information

dimethyladenosine transferase 1, mitochondrial

NCBI Summary for TFB1M

The protein encoded by this gene is a dimethyltransferase that methylates the conserved stem loop of mitochondrial 12S rRNA. The encoded protein also is part of the basal mitochondrial transcription complex and is necessary for mitochondrial gene expression. The methylation and transcriptional activities of this protein are independent of one another. Variations in this gene may influence the severity of aminoglycoside-induced deafness (AID).[provided by RefSeq, Aug 2010]

UniProt Comments for TFB1M

S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity.

Research Articles on TFB1M

1. Loss of TFB1M results in mitochondrial dysfunction that leads to impaired insulin secretion and diabetes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TFB1M recombinant protein

Reference Product	PubMed Publications
POLRMT recombinant protein	>9 publications with TFB1M and POLRMT
NRF1 recombinant protein	>4 publications with TFB1M and NRF1

Pathways associated with TFB1M recombinant protein

Products by Pathway	Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Mitochondrial Gene Expression Pathway antibodies	Mitochondrial Gene Expression Pathway Diagram
Mitochondrial Biogenesis Pathway antibodies	Mitochondrial Biogenesis Pathway Diagram
Organelle Biogenesis And Maintenance Pathway antibodies	Organelle Biogenesis And Maintenance Pathway Diagram
Transcriptional Activation Of Mitochondrial Biogenesis Pathway antibodies	Transcriptional Activation Of Mitochondrial Biogenesis Pathway Diagram
RRNA Modification In The Mitochondrion Pathway antibodies	RRNA Modification In The Mitochondrion Pathway Diagram
RRNA Processing Pathway antibodies	RRNA Processing Pathway Diagram
RRNA Processing In The Mitochondrion Pathway antibodies	RRNA Processing In The Mitochondrion Pathway Diagram

Diseases associated with TFB1M recombinant protein

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>4 publications with TFB1M and Cardiovascular Diseases
Disease Models, Animal Antibodies	>4 publications with TFB1M and Disease Models, Animal
Heart Diseases Antibodies	>3 publications with TFB1M and Heart Diseases
Inflammation Antibodies	>2 publications with TFB1M and Inflammation
Neurobehavioral Manifestations Antibodies	>1 publications with TFB1M and Neurobehavioral Manifestations
Neurodegenerative Diseases Antibodies	>1 publications with TFB1M and Neurodegenerative Diseases
Cardiomegaly Antibodies	>1 publications with TFB1M and Cardiomegaly
Hypertrophy Antibodies	>1 publications with TFB1M and Hypertrophy
Non-alcoholic Fatty Liver Disease Antibodies	>1 publications with TFB1M and Non-alcoholic Fatty Liver Disease
Fatty Liver Antibodies	>1 publications with TFB1M and Fatty Liver

Organs/Tissues associated with TFB1M recombinant protein

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>4 publications with TFB1M and Muscle
Brain Antibodies	>4 publications with TFB1M and Brain
Blood Antibodies	>3 publications with TFB1M and Blood
Pancreas Antibodies	>2 publications with TFB1M and Pancreas
Heart Antibodies	>2 publications with TFB1M and Heart
Mammary Gland Antibodies	>1 publications with TFB1M and Mammary Gland
Uterus Antibodies	>1 publications with TFB1M and Uterus
Embryonic Tissue Antibodies	>1 publications with TFB1M and Embryonic Tissue
Lung Antibodies	>1 publications with TFB1M and Lung
Liver Antibodies	>1 publications with TFB1M and Liver