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LCMT1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204231

LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A. Recombinant human LCMT7 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LCMT1, Recombinant Protein
Full Product Name
LCMT1, 1-334aa, Human, His tag E Coli
Product Gene Name
Product Synonym Names
Leucine carboxyl methyltransferase 1; CGI-68; LCMT; PPMT1
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMATRQR ESSITSCCST SSCDADDEGV RGTCEDASLC KRFAVSIGYW HDPYIQHFVR LSKERKAPEI NRGYFARVHG VSQLIKAFLR KTECHCQIVN LGAGMDTTFW RLKDEDLLPS KYFEVDFPMI VTRKLHSIKC KPPLSSPILE LHSEDTLQMD GHILDSKRYA VIGADLRDLS ELEEKLKKCN MNTQLPTLLI AECVLVYMTP EQSANLLKWA ANSFERAMFI NYEQVNMGDR FGQIMIENLR RRQCDLAGVE TCKSLESQKE RLLSNGWETA SAVDMMELYN RLPRAEVSRI ESLEFLDEME LLEQLMRHYC LCWATKGGNE LGLKEITY
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.
Purity/Purification
> 95% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
41 kDa (358aa) confirmed by MALDI-TOF
OMIM
AF037601 mRNA
3D Structure
ModBase 3D Structure for Q9UIC8
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for LCMT1 recombinant protein

Applications Tested/Suitable for LCMT1 recombinant protein
SDS-PAGE

Product References and Citations for LCMT1 recombinant protein

Lee JA., et al. (2007) J Biol Chem. 282 (42):30974-84.; De Baere I., et al. (1999) Biochemistry. 38 (50):16539-47.

Other Notes

Small volumes of LCMT1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LCMT1 purchase

MBS204231 is a recombinant protein targeting LCMT1. Also known as LCMT1, 1-334aa, Human, His tag E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for LCMT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
leucine carboxyl methyltransferase 1 isoform a
NCBI Official Synonym Full Names
leucine carboxyl methyltransferase 1
NCBI Official Symbol
LCMT1
NCBI Official Synonym Symbols
LCMT; PPMT1; CGI-68
UniProt Protein Name
Leucine carboxyl methyltransferase 1
UniProt Synonym Protein Names
Protein-leucine O-methyltransferase; [Phosphatase 2A protein]-leucine-carboxy methyltransferase 1
UniProt Gene Name
LCMT1
UniProt Synonym Gene Names
LCMT
NCBI Protein Information
leucine carboxyl methyltransferase 1

NCBI Summary for LCMT1

LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]).[supplied by OMIM, Mar 2008]

UniProt Comments for LCMT1

Methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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