mdh Active Protein

Name: mdh Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS204251
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204251

Malate dehydrogenase (mdh) belongs to the LDH/MDH superfamily and MDH type 1 family. This enzyme catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is also involved in… More details

$230 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

mdh, Active Protein

Full Product Name

mdh, 1-312aa, E Coli, His tag, E Coli (Bioactivity Validated)

Product Gene Name

mdh active protein

Product Synonym Names

malate dehydrogenase; ECK3225; JW3205

Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNK

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl

Purity/Purification

> 95% by SDS-PAGE

Concentration

1mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

34.9kDa (336aa), confirmed by MALDI-TOF

3D Structure

ModBase 3D Structure for P61889

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

E Coli

Tag Information

His-tag

Biological Activity

Specific activity is > 1000 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Assay

1. Prepare a 180ul assay buffer into a suitable container. The concentrations are 100 mM potassium phosphate (pH8.0), 0.5mM beta-nicotinamide adenine dinucleotide, reduced form, 0.5mM oxalacetic acid
2. Equilibrate to 37C and monitor at A340nm until the value is constant using a spectrophotometer.
3. Add 20ul of recombinant MDH protein 0.25ug/ml in assay buffer.
4. Mix by inversion and record the decrease at A340nm for 5 minutes.

Product Categories/Family for mdh active protein

Metabolism

Applications Tested/Suitable for mdh active protein

SDS-PAGE

Product References and Citations for mdh active protein

Goward CR., et al. (1994). Protein Sci. 3 (10): 1883-8.; McAlister-Henn L., et al. (1988). Trends Biochem. Sci. 13 (5): 178-81.

Other Notes

Small volumes of mdh active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for mdh purchase

MBS204251 is an active protein targeting mdh. Also known as mdh, 1-312aa, E Coli, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for mdh. It may not necessarily be applicable to this product.

NCBI GI #

16131126

NCBI GeneID

947854

NCBI Accession #

NP_417703

UniProt Primary Accession #

P61889

UniProt Secondary Accession #

O30401; O30402; O30403; P06994; Q2M8X7; Q59343; Q59344; Q59345; Q59346; Q59347; Q59348

GenBank Nucleotide #

NC_000913.3

NCBI Official Full Name

malate dehydrogenase, NAD(P)-binding

NCBI Official Symbol

mdh

NCBI Official Synonym Symbols

ECK3225; JW3205

UniProt Protein Name

Malate dehydrogenase

Protein Family

L-2-hydroxycarboxylate dehydrogenase

UniProt Gene Name

mdh

NCBI Protein Information

malate dehydrogenase, NAD(P)-binding

NCBI Summary for mdh

Mdh is elevated 4X during growth at 4C (enabled by Oleispira antarctica Cpn10/60 transgene). [More information is available at EcoGene: EG10576]. Malate dehydrogenase (Mdh) catalyzes the reversible oxidation of malate to generate oxaloacetate. [More information is available at EcoCyc: EG10576].

UniProt Comments for mdh

Catalyzes the reversible oxidation of malate to oxaloacetate.

Research Articles on mdh

1. The acetylation of lysine residues in MDH could enhance its enzyme activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with mdh active protein

Reference Product	PubMed Publications
ppc active protein	>10 publications with mdh and ppc
gltA active protein	>9 publications with mdh and gltA
fumC active protein	>6 publications with mdh and fumC
sucC active protein	>4 publications with mdh and sucC
aceB active protein	>3 publications with mdh and aceB
pckA active protein	>2 publications with mdh and pckA
fumB active protein	>1 publications with mdh and fumB

Pathways associated with mdh active protein

Products by Pathway	Pathway Diagram
Biosynthesis Of Antibiotics Pathway antibodies	Biosynthesis Of Antibiotics Pathway Diagram
Biosynthesis Of Secondary Metabolites Pathway antibodies	Biosynthesis Of Secondary Metabolites Pathway Diagram
CAM (Crassulacean Acid Metabolism), Dark Pathway antibodies	CAM (Crassulacean Acid Metabolism), Dark Pathway Diagram
CAM (Crassulacean Acid Metabolism), Dark Pathway antibodies	CAM (Crassulacean Acid Metabolism), Dark Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Citrate Cycle (TCA Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle) Pathway Diagram
Citrate Cycle (TCA Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle) Pathway Diagram
Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram
Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram