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POLB Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204252

DNA polymerase beta, also known as POLB, belongs to the DNA polymerase type-X family. In eukaryotic cells, DNA polymerase beta (POLB) performs base excision repair (BER) required for DNA maintenance, replication, recombination, and drug resistance. POLB has… More details

$230 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
POLB, Recombinant Protein
Full Product Name
POLB, 1-335aa, Human, His tag, E Coli
Product Gene Name
POLB recombinant protein
Product Synonym Names
DNA polymerase beta; DNA directed DNA polymerase beta; DNA pol beta; DNA polymerase beta; DNA polymerase beta subunit; MGC125976; Pol B; Pol beta; PolB; Polymerase (DNA directed) beta
Sequence
MGSSHHHHHH SSGLVPRGSH MSKRKAPQET LNGGITDMLT ELANFEKNVS QAIHKYNAYR KAASVIAKYP HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL TRVSGIGPSA ARKFVDEGIK TLEDLRKNED KLNHHQRIGL KYFGDFEKRI PREEMLQMQD IVLNEVKKVD SEYIATVCGS FRRGAESSGD MDVLLTHPSF TSESTKQPKL LHQVVEQLQK VHFITDTLSK GETKFMGVCQ LPSKNDEKEY PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP LGVTGVAGEP LPVDSEKDIF DYIQWKYREP KDRSE
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl
Purity/Purification
> 95% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
40.3 kDa (355aa), confirmed by MALDI-TOF
OMIM
L11607 mRNA
3D Structure
ModBase 3D Structure for P06746
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for POLB recombinant protein

Enzymes & Proteases
Applications Tested/Suitable for POLB recombinant protein
SDS-PAGE

SDS-Page of POLB recombinant protein

POLB Recombinant Protein – Cat# MBS204252

Product References and Citations for POLB recombinant protein

Bambara R A., et al. (1991) Biochim Biophys Acta. 1088:11-24.; Ropp P A., et al. (1996) Genomics. 36:449-458.

Other Notes

Small volumes of POLB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLB purchase

MBS204252 is a recombinant protein targeting POLB. Also known as POLB, 1-335aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for POLB. It may not necessarily be applicable to this product.

NCBI GI #
4505931
NCBI GeneID
5423
NCBI Accession #
NP_002681
UniProt Primary Accession #
P06746
UniProt Secondary Accession #
Q3KP48; Q6FI34; B2RC78
UniProt Related Accession #
P06746
GenBank Nucleotide #
NM_002690.2
NCBI Official Full Name
DNA polymerase beta
NCBI Official Synonym Full Names
DNA polymerase beta
NCBI Official Symbol
POLB
UniProt Protein Name
DNA polymerase beta
Protein Family
DNA polymerase
UniProt Gene Name
POLB
NCBI Protein Information
DNA polymerase beta

NCBI Summary for POLB

The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]
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UniProt Comments for POLB

Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.
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Research Articles on POLB

1. POLB, is located in the mitochondria and plays a significant role in mitochondrial BER, mtDNA integrity and mitochondrial function. [REVIEW]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with POLB recombinant protein

 Reference Product  PubMed Publications
 APEX1 recombinant protein  >13 publications with POLB and APEX1
 XRCC1 recombinant protein  >13 publications with POLB and XRCC1
 PCNA recombinant protein  >12 publications with POLB and PCNA
 LIG3 recombinant protein  >10 publications with POLB and LIG3
 FEN1 recombinant protein  >4 publications with POLB and FEN1
 NEIL1 recombinant protein  >3 publications with POLB and NEIL1
 LIG1 recombinant protein  >3 publications with POLB and LIG1
 PARP1 recombinant protein  >3 publications with POLB and PARP1

Pathways associated with POLB recombinant protein

 Products by Pathway  Pathway Diagram
 APEX1-Independent Resolution Of AP Sites Via The Single Nucleotide Replacement Pathway antibodies  APEX1-Independent Resolution Of AP Sites Via The Single Nucleotide Replacement Pathway Diagram
 Abasic Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway antibodies  Abasic Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway Diagram
 BER Complex Pathway antibodies  BER Complex Pathway Diagram
 BER Complex Pathway antibodies  BER Complex Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Deubiquitination Pathway antibodies  Deubiquitination Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram

Diseases associated with POLB recombinant protein

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >13 publications with POLB and Carcinoma
 Lung Neoplasms Antibodies  >6 publications with POLB and Lung Neoplasms
 Chromosome Aberrations Antibodies  >5 publications with POLB and Chromosome Aberrations
 Urinary Bladder Neoplasms Antibodies  >5 publications with POLB and Urinary Bladder Neoplasms
 Cell Transformation, Neoplastic Antibodies  >3 publications with POLB and Cell Transformation, Neoplastic
 Breast Neoplasms Antibodies  >3 publications with POLB and Breast Neoplasms
 Hyperplasia Antibodies  >2 publications with POLB and Hyperplasia
 Pancreatic Neoplasms Antibodies  >2 publications with POLB and Pancreatic Neoplasms
 Atherosclerosis Antibodies  >2 publications with POLB and Atherosclerosis
 Anemia Antibodies  >2 publications with POLB and Anemia

Organs/Tissues associated with POLB recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >8 publications with POLB and Lung
 Blood Antibodies  >5 publications with POLB and Blood
 Brain Antibodies  >4 publications with POLB and Brain
 Liver Antibodies  >3 publications with POLB and Liver
 Eye Antibodies  >2 publications with POLB and Eye
 Embryonic Tissue Antibodies  >2 publications with POLB and Embryonic Tissue
 Stomach Antibodies  >2 publications with POLB and Stomach
 Vascular Antibodies  >2 publications with POLB and Vascular
 Lymph Node Antibodies  >2 publications with POLB and Lymph Node
 Lymph Antibodies  >2 publications with POLB and Lymph
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