GSR Active Protein

Name: GSR Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS204266
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204266

Glutathione reductase, also known as GSR, is a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein and plays a role in maintaining glutathione (GSH) in its reduced form by catalyzing the… More details

$230 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GSR, Active Protein

Full Product Name

GSR, 43-522aa, Human, His tag, E Coli (Bioactivity Validated)

Product Gene Name

GSR active protein

Product Synonym Names

Glutathione reductase; GLuR; GRD1

Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAMACRQ EPQPQGPPPA AGAVASYDYL VIGGGSGGLA SARRAAELGA RAAVVESHKL GGTCVNVGCV PKKVMWNTAV HSEFMHDHAD YGFPSCEGKF NWRVIKEKRD AYVSRLNAIY QNNLTKSHIE IIRGHAAFTS DPKPTIEVSG KKYTAPHILI ATGGMPSTPH ESQIPGASLG ITSDGFFQLE ELPGRSVIVG AGYIAVEMAG ILSALGSKTS LMIRHDKVLR SFDSMISTNC TEELENAGVE VLKFSQVKEV KKTLSGLEVS MVTAVPGRLP VMTMIPDVDC LLWAIGRVPN TKDLSLNKLG IQTDDKGHII VDEFQNTNVK GIYAVGDVCG KALLTPVAIA AGRKLAHRLF EYKEDSKLDY NNIPTVVFSH PPIGTVGLTE DEAIHKYGIE NVKTYSTSFT PMYHAVTKRK TKCVMKMVCA NKEEKVVGIH MQGLGCDEML QGFAVAVKMG ATKADFDNTV AIHPTSSEEL VTLR

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Purity/Purification

> 95% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

54.3 kDa (504aa) confirmed by MALDI-TOF

OMIM

X15722 mRNA

3D Structure

ModBase 3D Structure for P00390

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Human

Tag Information

His-tag

Biological Activity

Specific activity is > 45 units/mg. The unit definition for glutathione reductase activity may be expressed in terms of the oxidation of NADPH or the reduction of GSSG since their molar ratio is 1:1. One unit of glutathione reductase oxidizes 1 umol of NADPH per minute at 37C, pH 7.5.

Product Categories/Family for GSR active protein

Redox Proteins

Applications Tested/Suitable for GSR active protein

SDS-PAGE

Product References and Citations for GSR active protein

Stoll V S., et al. (1997) Biochemistry. 36:6437-6447.; Karplus P A., et al. (1987) J Mol biol. 195:701-729.

Other Notes

Small volumes of GSR active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GSR purchase

MBS204266 is an active protein targeting GSR. Also known as GSR, 43-522aa, Human, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for GSR. It may not necessarily be applicable to this product.

NCBI GI #

50301238

NCBI GeneID

2936

NCBI Accession #

NP_000628

UniProt Primary Accession #

P00390

UniProt Secondary Accession #

Q7Z5C9; Q9NP63; C8KIL8; C8KIL9; C8KIM0; D3DSV3

UniProt Related Accession #

P00390

GenBank Nucleotide #

NM_000637.4

NCBI Official Full Name

glutathione reductase, mitochondrial isoform 1

NCBI Official Synonym Full Names

glutathione-disulfide reductase

NCBI Official Symbol

GSR

NCBI Official Synonym Symbols

HEL-75; HEL-S-122m

UniProt Protein Name

Glutathione reductase, mitochondrial

Protein Family

Glutathione reductase

UniProt Gene Name

GSR

UniProt Synonym Gene Names

GLUR; GRD1; GR; GRase

NCBI Protein Information

glutathione reductase, mitochondrial

NCBI Summary for GSR

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2010]

UniProt Comments for GSR

Maintains high levels of reduced glutathione in the cytosol.

Research Articles on GSR

1. GSR expression was higher in TMZ-resistant cells than in sensitive cells. GSR silencing in drug-resistant cells improved the sensitivity of cells to TMZ or cisplatin. Over-expression resulted in resistance. GSR partially prevented the oxidative stress caused by L-buthionine -sulfoximine. The action of GSR in drug resistance is associated with the modulation of redox homeostasis. High GSR correlated with lower survival.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GSR active protein

Reference Product	PubMed Publications
CAT active protein	>69 publications with GSR and CAT
GPX1 active protein	>28 publications with GSR and GPX1
GSS active protein	>15 publications with GSR and GSS
TXN active protein	>12 publications with GSR and TXN
GPX4 active protein	>10 publications with GSR and GPX4
GPX2 active protein	>7 publications with GSR and GPX2
GPX3 active protein	>5 publications with GSR and GPX3
GPX7 active protein	>3 publications with GSR and GPX7

Pathways associated with GSR active protein

Products by Pathway	Pathway Diagram
Amino Acid Metabolism Pathway antibodies	Amino Acid Metabolism Pathway Diagram
Cellular Responses To Stress Pathway antibodies	Cellular Responses To Stress Pathway Diagram
Detoxification Of Reactive Oxygen Species Pathway antibodies	Detoxification Of Reactive Oxygen Species Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Generic Transcription Pathway antibodies	Generic Transcription Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Amino Acids And Derivatives Pathway antibodies	Metabolism Of Amino Acids And Derivatives Pathway Diagram

Diseases associated with GSR active protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>83 publications with GSR and Nervous System Diseases
Cardiovascular Diseases Antibodies	>57 publications with GSR and Cardiovascular Diseases
Liver Diseases Antibodies	>27 publications with GSR and Liver Diseases
Inflammation Antibodies	>23 publications with GSR and Inflammation
Disease Models, Animal Antibodies	>19 publications with GSR and Disease Models, Animal
Hypertension Antibodies	>19 publications with GSR and Hypertension
Necrosis Antibodies	>18 publications with GSR and Necrosis
Poisoning Antibodies	>16 publications with GSR and Poisoning
Drug-Related Side Effects and Adverse Reactions Antibodies	>13 publications with GSR and Drug-Related Side Effects and Adverse Reactions
Heart Diseases Antibodies	>12 publications with GSR and Heart Diseases

Organs/Tissues associated with GSR active protein

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>512 publications with GSR and Skin
Blood Antibodies	>181 publications with GSR and Blood
Heart Antibodies	>132 publications with GSR and Heart
Brain Antibodies	>105 publications with GSR and Brain
Liver Antibodies	>54 publications with GSR and Liver
Kidney Antibodies	>33 publications with GSR and Kidney
Vascular Antibodies	>30 publications with GSR and Vascular
Nerve Antibodies	>24 publications with GSR and Nerve
Eye Antibodies	>24 publications with GSR and Eye
Lung Antibodies	>23 publications with GSR and Lung