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CNOT7 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204435

CNOT7, also known as CCR4-Not transcription complex, subunit 7, is a ubiquitous transcription factor. It is a component of the CCR4 complex which functions as a general transcription regulation complex. CNOT7 also binds to an anti-proliferative protein,… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CNOT7, Recombinant Protein
Full Product Name
CNOT7, 1-248aa, Mouse, His tag, E Coli
Product Gene Name
Product Synonym Names
CCR4-Not transcription complex subunit 7; CCR4-Not transcription complex; subunit 7; CAF-1
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMPAATVD HSQRICEVWA CNLDEEMKKI RQVIRKYNYV AMDTEFPGVV ARPIGEFRSN ADYQYQLLRC NVDLLKIIQL GLTFMNEQGE YPPGTSTWQF NFKFNLTEDM YAQDSIELLT TSGIQFKKHE EEGIETQYFA ELLMTSGVVL CEGVKWLSFH SGYDFGYLIK ILTNSNLPEE ELDFFEILRL FFPVIYDVKY LMKSCKNLKM FFEDHIDDAK YCGHLYGLGS GSSYVQNGTG NAYEEEASKQ S
Host
E Coli
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 1mM DTT
Purity/Purification
> 80% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
31.1 kDa (271aa), confirmed by MALDI-TOF
Antigen Species
Mouse
Tag Information
His-tag
Applications Tested/Suitable for CNOT7 recombinant protein
SDS-PAGE

Product References and Citations for CNOT7 recombinant protein

Draper M.P., et al. (1995) Mol. Cell. Biol. 15:3487-3495; Bogdan JA, et al. (1998) Biochem J. 336 (Pt 2):471-81

Other Notes

Small volumes of CNOT7 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CNOT7 purchase

MBS204435 is a recombinant protein targeting CNOT7. Also known as CNOT7, 1-248aa, Mouse, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CNOT7. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
NCBI Official Full Name
unnamed protein product
NCBI Official Synonym Full Names
CCR4-NOT transcription complex, subunit 7
NCBI Official Symbol
Cnot7
NCBI Official Synonym Symbols
Caf1; Pop2; CAF-1; AU022737
UniProt Protein Name
CCR4-NOT transcription complex subunit 7
UniProt Synonym Protein Names
CCR4-associated factor 1; CAF-1
UniProt Gene Name
Cnot7
UniProt Synonym Gene Names
Caf1; CAF-1
NCBI Protein Information
CCR4-NOT transcription complex subunit 7

UniProt Comments for CNOT7

Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for miRNA-mediated mRNA deadenylation. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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