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MYL12B Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204456

Myosin, light chain 12B, regulatory, also known as MYL12B, is a hexameric ATPase cellular motor protein. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL12B regulates… More details

$230 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MYL12B, Recombinant Protein
Full Product Name
MYL12B, 1-172aa, Human, His tag, E Coli
Product Synonym Names
Myosin light chain 12B regulatory; Myosin; light chain 12B regulatory; MLC-B; MRLC2
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSSKKA KTKTTKKRPQ RATSNVFAMF DQSQIQEFKE AFNMIDQNRD GFIDKEDLHD MLASLGKNPT DAYLDAMMNE APGPINFTMF LTMFGEKLNG TDPEDVIRNA FACFDEEATG TIQEDYLREL LTTMGDRFTD EEVDELYREA PIDKKGNFNY IEFTRILKHG AKDKDD
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT
Purity/Purification
> 90% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
22.3 kDa (196aa) confirmed by MALDI-TOF
3D Structure
ModBase 3D Structure for O14950
Antigen Species
Human
Tag Information
His-tag
Applications Tested/Suitable for MYL12B recombinant protein
SDS-PAGE

Product References and Citations for MYL12B recombinant protein

Kumar C C., et al. (1989) Biochemistry. 28:4027-4035.; Kolodney M S., et al. (1999) J Physiol. 515: 87-92.

Other Notes

Small volumes of MYL12B recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MYL12B purchase

MBS204456 is a recombinant protein targeting MYL12B. Also known as MYL12B, 1-172aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for MYL12B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
myosin regulatory light chain 12B
NCBI Official Synonym Full Names
myosin light chain 12B
NCBI Official Symbol
MYL12B
NCBI Official Synonym Symbols
MLC-B; MRLC2
UniProt Protein Name
Myosin regulatory light chain 12B
UniProt Synonym Protein Names
MLC-2A; MLC-2; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; MLC20; Myosin regulatory light chain MRLC2; SHUJUN-1
Protein Family
UniProt Gene Name
MYL12B
UniProt Synonym Gene Names
MRLC2; MYLC2B; MLC-2; MLC20
NCBI Protein Information
myosin regulatory light chain 12B

NCBI Summary for MYL12B

The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM, Mar 2008]
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UniProt Comments for MYL12B

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with MYL12B recombinant protein

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