IFI30 Recombinant Protein

IFI30, also as known as gamma-interferon-inducible lysosomal thiol reductase, belongs to the GILT family. This protein is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. This protein facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or cross-presentation. Recombinant human IFI30 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Arunachalam B, et al. (2000) Proc Natl Acad Sci u S A 97 (2): 745-50. ; Luster AD, et al. (1988) J Biol Chem 263 (24): 12036-43.

Small volumes of IFI30 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS204459 is a recombinant protein targeting IFI30. Also known as IFI30, 58-232aa, Human, His tag, E Coli. Host: E Coli.