TFB2M Recombinant Protein

Name: TFB2M Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS204492
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204492

TFB2M is S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. This protein is also required for basal transcription of mitochondrial DNA, probably via its interaction with… More details

$245 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TFB2M, Recombinant Protein

Full Product Name

TFB2M, 20-396aa, Human, His tag, E Coli

Product Gene Name

TFB2M recombinant protein

Product Synonym Names

Dimethyladenosine transferase 2 mitochondrial; Dimethyladenosine transferase 2; mitochondrial; Hkp1; mtTFB2

Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAGRFCI LGSEAATRKH LPARNHCGLS DSSPQLWPEP DFRNPPRKAS KASLDFKRYV TDRRLAETLA QIYLGKPSRP PHLLLECNPG PGILTQALLE AGAKVVALES DKTFIPHLES LGKNLDGKLR VIHCDFFKLD PRSGGVIKPP AMSSRGLFKN LGIEAVPWTA DIPLKVVGMF PSRGEKRALW KLAYDLYSCT SIYKFGRIEV NMFIGEKEFQ KLMADPGNPD LYHVLSVIWQ LACEIKVLHM EPWSSFDIYT RKGPLENPKR RELLDQLQQK LYLIQMIPRQ NLFTKNLTPM NYNIFFHLLK HCFGRRSATV IDHLRSLTPL DARDILMQIG KQEDEKVVNM HPQDFKTLFE TIERSKDCAY KWLYDETLED R

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Purity/Purification

> 90% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

45.8 kDa (401aa) confirmed by MALDI-TOF

OMIM

AF529366 mRNA

3D Structure

ModBase 3D Structure for Q9H5Q4

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for TFB2M recombinant protein

Metabolism

Applications Tested/Suitable for TFB2M recombinant protein

SDS-PAGE

Product References and Citations for TFB2M recombinant protein

Litonin,D.,et al. (2010) J. Biol. Chem. 285 (24), 18129-18133; Norrbom,J.,et al. (2010) Acta Physiol (Oxf) 198 (1), 71-79.

Other Notes

Small volumes of TFB2M recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TFB2M purchase

MBS204492 is a recombinant protein targeting TFB2M. Also known as TFB2M, 20-396aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for TFB2M. It may not necessarily be applicable to this product.

NCBI GI #

11641289

NCBI GeneID

64216

NCBI Accession #

NP_071761

UniProt Primary Accession #

Q9H5Q4

UniProt Secondary Accession #

Q9H626

UniProt Related Accession #

Q9H5Q4

GenBank Nucleotide #

NM_022366.2

NCBI Official Full Name

dimethyladenosine transferase 2, mitochondrial

NCBI Official Synonym Full Names

transcription factor B2, mitochondrial

NCBI Official Symbol

TFB2M

NCBI Official Synonym Symbols

Hkp1; mtTFB2

UniProt Protein Name

Dimethyladenosine transferase 2, mitochondrial

UniProt Synonym Protein Names

Hepatitis C virus NS5A-transactivated protein 5; HCV NS5A-transactivated protein 5; Mitochondrial 12S rRNA dimethylase 2; Mitochondrial transcription factor B2; h-mtTFB; h-mtTFB2; hTFB2M; mtTFB2; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2

Protein Family

Dimethyladenosine transferase

UniProt Gene Name

TFB2M

UniProt Synonym Gene Names

NS5ATP5; HCV NS5A-transactivated protein 5; h-mtTFB; h-mtTFB2; hTFB2M; mtTFB2

NCBI Protein Information

dimethyladenosine transferase 2, mitochondrial

UniProt Comments for TFB2M

S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.

Research Articles on TFB2M

1. Study reports crystal structures of human mitochondrial transcription initiation complexes assembled on both light and heavy strand promoters. The structures reveal how transcription factors TFAM and TFB2M assist mitochondrial RNA polymerase to achieve promoter-dependent initiation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TFB2M recombinant protein

Reference Product	PubMed Publications
TFAM recombinant protein	>53 publications with TFB2M and TFAM
POLRMT recombinant protein	>24 publications with TFB2M and POLRMT

Pathways associated with TFB2M recombinant protein

Products by Pathway	Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Mitochondrial Gene Expression Pathway antibodies	Mitochondrial Gene Expression Pathway Diagram
Mitochondrial Biogenesis Pathway antibodies	Mitochondrial Biogenesis Pathway Diagram
Mitochondrial Transcription Initiation Pathway antibodies	Mitochondrial Transcription Initiation Pathway Diagram
Organelle Biogenesis And Maintenance Pathway antibodies	Organelle Biogenesis And Maintenance Pathway Diagram
RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies	RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
Transcription From Mitochondrial Promoters Pathway antibodies	Transcription From Mitochondrial Promoters Pathway Diagram
Transcriptional Activation Of Mitochondrial Biogenesis Pathway antibodies	Transcriptional Activation Of Mitochondrial Biogenesis Pathway Diagram

Diseases associated with TFB2M recombinant protein

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>4 publications with TFB2M and Cardiovascular Diseases
Nervous System Diseases Antibodies	>4 publications with TFB2M and Nervous System Diseases
Heart Diseases Antibodies	>3 publications with TFB2M and Heart Diseases
Fatty Liver Antibodies	>2 publications with TFB2M and Fatty Liver
Hypertrophy Antibodies	>2 publications with TFB2M and Hypertrophy
Non-alcoholic Fatty Liver Disease Antibodies	>2 publications with TFB2M and Non-alcoholic Fatty Liver Disease
Inflammation Antibodies	>2 publications with TFB2M and Inflammation
Embryo Loss Antibodies	>1 publications with TFB2M and Embryo Loss
Myocardial Infarction Antibodies	>1 publications with TFB2M and Myocardial Infarction
Liver Diseases Antibodies	>1 publications with TFB2M and Liver Diseases

Organs/Tissues associated with TFB2M recombinant protein

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>13 publications with TFB2M and Muscle
Blood Antibodies	>5 publications with TFB2M and Blood
Brain Antibodies	>4 publications with TFB2M and Brain
Heart Antibodies	>3 publications with TFB2M and Heart
Liver Antibodies	>3 publications with TFB2M and Liver
Mammary Gland Antibodies	>1 publications with TFB2M and Mammary Gland
Uterus Antibodies	>1 publications with TFB2M and Uterus
Lung Antibodies	>1 publications with TFB2M and Lung
Embryonic Tissue Antibodies	>1 publications with TFB2M and Embryonic Tissue
Eye Antibodies	>1 publications with TFB2M and Eye