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CTH Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204550

CTH (Cystathionine gamma-lyase or cystathionase) belongs to the trans-sulfuration enzymes family. This protein is an enzyme which breaks down cystathionine into cysteine and alpha-ketobutyrate. CTH catalyzes the last step in the transsulfuration pathway from… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CTH, Recombinant Protein
Full Product Name
CTH, 1-405aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
Cystathionine gamma-lyase; Cystathionine gamma-lyase; Gamma-cystathionase
Sequence
MGSSHHHHHH SSGLVPRGSH MQEKDASSQG FLPHFQHFAT QAIHVGQDPE QWTSRAVVPP ISLSTTFKQG APGQHSGFEY SRSGNPTRNC LEKAVAALDG AKYCLAFASG LAATVTITHL LKAGDQIICM DDVYGGTNRY FRQVASEFGL KISFVDCSKI KLLEAAITPE TKLVWIETPT NPTQKVIDIE GCAHIVHKHG DIILVVDNTF MSPYFQRPLA LGADISMYSA TKYMNGHSDV VMGLVSVNCE SLHNRLRFLQ NSLGAVPSPI DCYLCNRGLK TLHVRMEKHF KNGMAVAQFL ESNPWVEKVI YPGLPSHPQH ELVKRQCTGC TGMVTFYIKG TLQHAEIFLK NLKLFTLAES LGGFESLAEL PAIMTHASVL KNDRDVLGIS DTLIRLSVGL EDEEDLLEDL DQALKAAHPP SGSHS
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100mM NaCl
Purity/Purification
> 95% by SDS-PAGE
Concentration
1mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
46.7kDa (425aa), confirmed by MALDI-TOF
OMIM
S52784 mRNA
3D Structure
ModBase 3D Structure for P32929
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for CTH recombinant protein

Applications Tested/Suitable for CTH recombinant protein
SDS-PAGE

Product References and Citations for CTH recombinant protein

Chiku T., et al. (2009) J. Biol. Chem. 284:11601-11612; Sun Q., et al. (2009) J. Biol. Chem. 284:3076-3085

Other Notes

Small volumes of CTH recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CTH purchase

MBS204550 is a recombinant protein targeting CTH. Also known as CTH, 1-405aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CTH. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cystathionine gamma-lyase isoform 1
NCBI Official Synonym Full Names
cystathionine gamma-lyase
NCBI Official Symbol
CTH
UniProt Protein Name
Cystathionine gamma-lyase
UniProt Synonym Protein Names
Cysteine-protein sulfhydrase; Gamma-cystathionase
UniProt Gene Name
CTH
NCBI Protein Information
cystathionine gamma-lyase

NCBI Summary for CTH

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]
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UniProt Comments for CTH

Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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