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NT5M Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204694

NT5M, also known as DNT2, belongs to the 5' (3')-deoxyribonucleotidase family. It localizes to the mitochondrial matrix. This enzyme dephosphorylates the 5'- and 2' (3')-phosphates of uracil and thymine deoxyribonucleotides. The gene is located on chromosome… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NT5M, Recombinant Protein
Full Product Name
NT5M, 32-228aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
5' (3')-deoxyribonucleotidase mitochondrial; 5' (3')-deoxyribonucleotidase; mitochondrial; DNT2
Sequence
MGSSHHHHHH SSGLVPRGSH MGGRALRVLV DMDGVLADFE GGFLRKFRAR FPDQPFIALE DRRGFWVSEQ YGRLRPGLSE KAISIWESKN FFFELEPLPG AVEAVKEMAS LQNTDVFICT SPIKMFKYCP YEKYAWVEKY FGPDFLEQIV LTRDKTVVSA DLLIDDRPDI TGAEPTPSWE HVLFTACHNQ HLQLQPPRRR LHSWADDWKA ILDSKRPC
Host
E Coli
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Purity/Purification
> 95% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
25.1 kDa (218aa), confirmed by MALDI-TOF
OMIM
AJ277557 Genomic DNA
3D Structure
ModBase 3D Structure for Q9NPB1
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for NT5M recombinant protein

Applications Tested/Suitable for NT5M recombinant protein
SDS-PAGE

Product References and Citations for NT5M recombinant protein

Rampazzo C., et al. (2000) Proc. Natl. Acad. Sci. u.S.A. 97:8239-8244; Rinaldo-Matthis A., et al. (2002) Nat. Struct. Biol. 9:779-787

Other Notes

Small volumes of NT5M recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NT5M purchase

MBS204694 is a recombinant protein targeting NT5M. Also known as NT5M, 32-228aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for NT5M. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
5'(3')-deoxyribonucleotidase, mitochondrial
NCBI Official Synonym Full Names
5',3'-nucleotidase, mitochondrial
NCBI Official Symbol
NT5M
NCBI Official Synonym Symbols
mdN; dNT2; dNT-2
UniProt Protein Name
5'(3')-deoxyribonucleotidase, mitochondrial
UniProt Synonym Protein Names
Deoxy-5'-nucleotidase 2; dNT-2
UniProt Gene Name
NT5M
UniProt Synonym Gene Names
DNT2; 5',3'-nucleotidase, mitochondrial; dNT-2
NCBI Protein Information
5'(3')-deoxyribonucleotidase, mitochondrial

NCBI Summary for NT5M

This gene encodes a 5' nucleotidase that localizes to the mitochondrial matrix. This enzyme dephosphorylates the 5'- and 2'(3')-phosphates of uracil and thymine deoxyribonucleotides. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

UniProt Comments for NT5M

Dephosphorylates specifically the 5' and 2'(3')-phosphates of uracil and thymine deoxyribonucleotides, and so protects mitochondrial DNA replication from excess dTTP. Has only marginal activity towards dIMP and dGMP.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NT5M recombinant protein

 Reference Product  PubMed Publications
 DGUOK recombinant protein  >1 publications with NT5M and DGUOK

Diseases associated with NT5M recombinant protein

Organs/Tissues associated with NT5M recombinant protein

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