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POu2AF1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204714

POu2AF1, also known as BOB1, is a lymphocyte specific transcription coactivator protein. This protein interacts only with the Oct1/2 proteins through sub domains in the POu domain of the Oct1/2 proteins, enhancing their transcriptional efficacy. Although… More details

$230 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POu2AF1, Recombinant Protein
Full Product Name
POu2AF1, 1-256aa, Human, His tag, E Coli
Product Synonym Names
POu domain class 2-associating factor 1; BOB1; OBF-1; OBF1; OCAB
Sequence
MGSSHHHHHH SSGLVPRGSH MLWQKPTAPE QAPAPARPYQ GVRVKEPVKE LLRRKRGHAS SGAAPAPTAV VLPHQPLATY TTVGPSCLDM EGSVSAVTEE AALCAGWLSQ PTPATLQPLA PWTPYTEYVP HEAVSCPYSA DMYVQPVCPS YTVVGPSSVL TYASPPLITN VTTRSSATPA VGPPLEGPEH QAPLTYFPWP QPLSTLPTST LQYQPPAPAL PGPQFVQLPI SIPEPVLQDM EDPRRAASSL TIDKLLLEEE DSDAYALNHT LSVEGF
Host
E Coli
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol
Purity/Purification
> 85% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
29.6 kDa (276aa), confirmed by MALDI-TOF (molecular weight on SDS-PAGE will appear higher)
OMIM
Z47550 mRNA
3D Structure
ModBase 3D Structure for Q16633
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for POu2AF1 recombinant protein

Applications Tested/Suitable for POu2AF1 recombinant protein
SDS-PAGE

Product References and Citations for POu2AF1 recombinant protein

Gstaiger M., et al. (1995) Nature. 373:360-362; Luo Y., et al. (1995) Mol. Cell. Biol. 15:4115-4124

Other Notes

Small volumes of POu2AF1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POu2AF1 purchase

MBS204714 is a recombinant protein targeting POu2AF1. Also known as POu2AF1, 1-256aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for POu2AF1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
POU domain class 2-associating factor 1
NCBI Official Synonym Full Names
POU class 2 associating factor 1
NCBI Official Symbol
POU2AF1
NCBI Official Synonym Symbols
BOB1; OBF1; OCAB; OBF-1
UniProt Protein Name
POU domain class 2-associating factor 1
UniProt Synonym Protein Names
B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1
UniProt Gene Name
POU2AF1
UniProt Synonym Gene Names
OBF1
NCBI Protein Information
POU domain class 2-associating factor 1

UniProt Comments for POu2AF1

Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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