VPS25 Recombinant Protein

Name: VPS25 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS204715
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204715

VPS25, also known as vacuolar protein-sorting-associated protein 25, consists of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into… More details

$230 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

VPS25, Recombinant Protein

Full Product Name

VPS25, 1-176aa, Human, His tag, E Coli

Product Gene Name

VPS25 recombinant protein

Product Synonym Names

Vacuolar protein-sorting-associated protein 25; DERP9; EAP20; FAP20

Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMAMSFE WPWQYRFPPF FTLQPNVDTR QKQLAAWCSL VLSFCRLHKQ SSMTVMEAQE SPLFNNVKLQ RKLPVESIQI VLEELRKKGN LEWLDKSKSS FLIMWRRPEE WGKLIYQWVS RSGQNNSVFT LYELTNGEDT EDEEFHGLDE ATLLRALQAL QQEHKAEIIT VSDGRGVKFF

Host

E Coli

Form/Format

Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol 0.1M NaCl

Purity/Purification

> 85% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

23.3 kDa (200aa), confirmed by MALDI-TOF

OMIM

AB014763 mRNA

3D Structure

ModBase 3D Structure for Q9BRG1

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for VPS25 recombinant protein

Signal Transduction

Applications Tested/Suitable for VPS25 recombinant protein

SDS-PAGE

Product References and Citations for VPS25 recombinant protein

Bailey,S.D. et al. (2010) Diabetes Care 33 (10), 2250-2253; Talmud,P.J. et al. (2009) Am. J. Hum. Genet. 85 (5), 628-642;

Other Notes

Small volumes of VPS25 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VPS25 purchase

MBS204715 is a recombinant protein targeting VPS25. Also known as VPS25, 1-176aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for VPS25. It may not necessarily be applicable to this product.

NCBI GI #

14150155

NCBI GeneID

84313

NCBI Accession #

NP_115729

UniProt Primary Accession #

Q9BRG1

UniProt Secondary Accession #

B2R581

UniProt Related Accession #

Q9BRG1

GenBank Nucleotide #

NM_032353.3

NCBI Official Full Name

vacuolar protein-sorting-associated protein 25

NCBI Official Synonym Full Names

vacuolar protein sorting 25 homolog

NCBI Official Symbol

VPS25

NCBI Official Synonym Symbols

DERP9; EAP20; FAP20

UniProt Protein Name

Vacuolar protein-sorting-associated protein 25

UniProt Synonym Protein Names

Dermal papilla-derived protein 9; ELL-associated protein of 20 kDa; ESCRT-II complex subunit VPS25

Protein Family

Vacuolar protein sorting-associated protein

UniProt Gene Name

VPS25

UniProt Synonym Gene Names

DERP9; EAP20; hVps25

NCBI Protein Information

vacuolar protein-sorting-associated protein 25

NCBI Summary for VPS25

This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Jul 2013]

UniProt Comments for VPS25

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.

Research Articles on VPS25

1. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with VPS25 recombinant protein

Reference Product	PubMed Publications
VPS36 recombinant protein	>11 publications with VPS25 and VPS36
SNF8 recombinant protein	>6 publications with VPS25 and SNF8
TSG101 recombinant protein	>5 publications with VPS25 and TSG101
VPS28 recombinant protein	>2 publications with VPS25 and VPS28
CHMP6 recombinant protein	>2 publications with VPS25 and CHMP6
CHMP3 recombinant protein	>1 publications with VPS25 and CHMP3
CHMP4B recombinant protein	>1 publications with VPS25 and CHMP4B

Pathways associated with VPS25 recombinant protein

Products by Pathway	Pathway Diagram
ESCRT-II Complex Pathway antibodies	ESCRT-II Complex Pathway Diagram
ESCRT-II Complex Pathway antibodies	ESCRT-II Complex Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Endosomal Sorting Complex Required For Transport (ESCRT) Pathway antibodies	Endosomal Sorting Complex Required For Transport (ESCRT) Pathway Diagram
Membrane Trafficking Pathway antibodies	Membrane Trafficking Pathway Diagram
Vesicle-mediated Transport Pathway antibodies	Vesicle-mediated Transport Pathway Diagram

Diseases associated with VPS25 recombinant protein

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>3 publications with VPS25 and Kidney Diseases
Disease Models, Animal Antibodies	>1 publications with VPS25 and Disease Models, Animal

Organs/Tissues associated with VPS25 recombinant protein

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>6 publications with VPS25 and Eye
Kidney Antibodies	>4 publications with VPS25 and Kidney
Lymph Antibodies	>1 publications with VPS25 and Lymph
Blood Antibodies	>1 publications with VPS25 and Blood
Testis Antibodies	>1 publications with VPS25 and Testis
Bone Antibodies	>1 publications with VPS25 and Bone
Uncharacterized Tissue Antibodies	>1 publications with VPS25 and Uncharacterized Tissue
Embryonic Tissue Antibodies	>1 publications with VPS25 and Embryonic Tissue