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uBE2F Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204718

uBE2F, also Known as ubiquitin-conjugating enzyme E2 F, belongs to the ubiquitin-conjugating enzyme family and uBE2F subfamily. ubiquitin-conjugating enzymes more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that… More details

$230 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
uBE2F, Recombinant Protein
Full Product Name
uBE2F, 1-185aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
NEDD8-conjugating enzyme uBE2F; NCE2
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMLTLAS KLKRDDGLKG SRTAATASDS TRRVSVRDKL LVKEVAELEA NLPCTCKVHF PDPNKLHCFQ LTVTPDEGYY QGGKFQFETE VPDAYNMVPP KVKCLTKIWH PNITETGEIC LSLLREHSID GTGWAPTRTL KDVVWGLNSL FTDLLNFDDP LNIEAAEHHL RDKEDFRNKV DDYIKRYAR
Host
E Coli
Form/Format
Liquid. In 20 mM Tris-HCl Buffer, pH8.0, 30% glycerol, 1mM DTT, 200mM NaCl
Purity/Purification
> 95% by SDS-PAGE
Concentration
1mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
23.7kDa (209aa) confirmed by MALDI-TOF
OMIM
AF310723 mRNA
3D Structure
ModBase 3D Structure for Q969M7
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for uBE2F recombinant protein

Applications Tested/Suitable for uBE2F recombinant protein
SDS-PAGE

Product References and Citations for uBE2F recombinant protein

Huang D.T., et al. (2009) Mol. Cell 33:483-495; Nandi, D. et al. (2006) Journal of biosciences 31 (1): 137-55.

Other Notes

Small volumes of uBE2F recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for uBE2F purchase

MBS204718 is a recombinant protein targeting uBE2F. Also known as uBE2F, 1-185aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for uBE2F. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
NEDD8-conjugating enzyme UBE2F isoform 1
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 F (putative)
NCBI Official Symbol
UBE2F
NCBI Official Synonym Symbols
NCE2
UniProt Protein Name
NEDD8-conjugating enzyme UBE2F
UniProt Synonym Protein Names
NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; RING-type E3 NEDD8 transferase UBE2F; Ubiquitin-conjugating enzyme E2 F
UniProt Gene Name
UBE2F
UniProt Synonym Gene Names
NCE2
NCBI Protein Information
NEDD8-conjugating enzyme UBE2F

UniProt Comments for uBE2F

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with uBE2F recombinant protein

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