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uBE2M Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204734

uBE2M, also known as NEDD8-conjugating enzyme ubc12, belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation.… More details

$230 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
uBE2M, Recombinant Protein
Full Product Name
uBE2M, 1-183aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
NEDD8-conjugating enzyme ubc12; hubc12; uBC-RS2; uBC12
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMIKLFS LKQQKKEEES AGGTKGSSKK ASAAQLRIQK DINELNLPKT CDISFSDPDD LLNFKLVICP DEGFYKSGKF VFSFKVGQGY PHDPPKVKCE TMVYHPNIDL EGNVCLNILR EDWKPVLTIN SIIYGLQYLF LEPNPEDPLN KEAAEVLQNN RRLFEQNVQR SMRGGYIGST YFERCLK
Host
E Coli
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.2M NaCl, 1mM DTT
Purity/Purification
> 95% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
23.5 kDa (207aa) confirmed by MALDI-TOF
OMIM
AB012191 mRNA
3D Structure
ModBase 3D Structure for P61081
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for uBE2M recombinant protein

Applications Tested/Suitable for uBE2M recombinant protein
SDS-PAGE

Product References and Citations for uBE2M recombinant protein

Gong L., et al. (1999) J. Biol. Chem. 274:12036-12042; Huang D.T., et al. (2004) Nat. Struct. Mol. Biol. 11:927-935

Other Notes

Small volumes of uBE2M recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for uBE2M purchase

MBS204734 is a recombinant protein targeting uBE2M. Also known as uBE2M, 1-183aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for uBE2M. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
NEDD8-conjugating enzyme Ubc12
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 M
NCBI Official Symbol
UBE2M
NCBI Official Synonym Symbols
UBC12; hUbc12; UBC-RS2
UniProt Protein Name
NEDD8-conjugating enzyme Ubc12
UniProt Synonym Protein Names
NEDD8 carrier protein; NEDD8 protein ligase; Ubiquitin-conjugating enzyme E2 M
UniProt Gene Name
UBE2M
UniProt Synonym Gene Names
UBC12
NCBI Protein Information
NEDD8-conjugating enzyme Ubc12

NCBI Summary for uBE2M

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008]
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UniProt Comments for uBE2M

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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