uBA5 Recombinant Protein

uBA5 (ubiquitin-like modifier activating enzyme 5) belongs to the ubiquitin-activating E1 family and uBA5 subfamily. ubiquitin and ubiquitin-like proteins are known to be covalently conjugated to a variety of cellular substrates via a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) plays a key role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. uBA5 had been proved to activate an ubiquitin-like protein, ubiquitin-fold modifier 1 (ufm1), by forming a high-energy thioester bond. It localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SuMO2. Recombinant human uBA5 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Komatsu M., et al. (2004) EMBO J. 23:1977-1986; Zheng M., et al. (2008) J. Cell. Biochem. 104:2324-2334

Small volumes of uBA5 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Ubiquitin enzymes tag proteins for ultimate hydrolysis in complex quality control pathways. Homologous proteins are expressed across species human mouse rat - and have recently emerged as key therapeutic targets for drugs compounds screening. Enzymes are activated by ubiquitin activating modifier activating proteins like activating enzyme uBA5. We provide a human HIS6 tagged, highly purified uBA5 recombinant protein, generated using uBA5 gene expression in E coli from Origene (license of function genomic views intellectual property are available upon request). Species humanapplications for this modifier activating enzyme uBA 3 recombinant product run from rabbit polyclonal antibody production to genes regulation, IHC positive control to families drugs compounds screens to enhance or reduce uFM1 activating enzyme 5 activity. MBS204777 is sold in economical 10 ug x 5 - 250 ug quantities making it a top value among enzyme uBA5 recombinant products seen in some PubMed ID publications. Reward points apply to purchase.