• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

POLR2D Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204821

POLR2D is the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress… More details

$230 / 0.02 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POLR2D, Recombinant Protein
Full Product Name
POLR2D, 1-142aa, Human, His tag, E Coli (Denatured)
Product Gene Name
POLR2D recombinant protein
Product Synonym Names
DNA-directed RNA polymerase II subunit RPB4; HSRBP4; HSRPB4; RBP4; RPB16
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAGGSD PRAGDVEEDA SQLIFPKEFE TAETLLNSEV HMLLEHRKQQ NESAEDEQEL SEVFMKTLNY TARFSRFKNR ETIASVRSLL LQKKLHKFEL ACLANLCPET AEESKALIPS LEGRFEDEEL QQILDDIQTK RSFQY
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea 10% glycerol
Purity/Purification
> 90% by SDS-PAGE
Concentration
1.0 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
18.7 kDa (165aa)
OMIM
U89387 Genomic DNA
3D Structure
ModBase 3D Structure for O15514
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for POLR2D recombinant protein

Epigenomics
Applications Tested/Suitable for POLR2D recombinant protein
SDS-PAGE
Application Notes for POLR2D recombinant protein
This protein is denatured. Denatured protein is most likely not the best option for functional studies. Denatured protein is better suited for Western Blot (WB) or imaging assays. Applications are user determined.

SDS-Page of POLR2D recombinant protein

POLR2D Recombinant Protein – Cat# MBS204821

Product References and Citations for POLR2D recombinant protein

Khazak V., et al. (1998). Mol. Cell. Biol. 18:1935-1945

Other Notes

Small volumes of POLR2D recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLR2D purchase

MBS204821 is a recombinant protein targeting POLR2D. Also known as POLR2D, 1-142aa, Human, His tag, E Coli (Denatured). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for POLR2D. It may not necessarily be applicable to this product.

NCBI GI #
4758574
NCBI GeneID
5433
NCBI Accession #
NP_004796
UniProt Primary Accession #
O15514
UniProt Secondary Accession #
Q52LT4
UniProt Related Accession #
O15514
GenBank Nucleotide #
NM_004805.3
NCBI Official Full Name
DNA-directed RNA polymerase II subunit RPB4
NCBI Official Synonym Full Names
RNA polymerase II subunit D
NCBI Official Symbol
POLR2D
NCBI Official Synonym Symbols
RBP4; RPB16; HSRBP4; HSRPB4
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB4
UniProt Synonym Protein Names
DNA-directed RNA polymerase II subunit D; RNA polymerase II 16 kDa subunit; RPB16
UniProt Gene Name
POLR2D
UniProt Synonym Gene Names
RNA polymerase II subunit B4; RPB16
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB4

NCBI Summary for POLR2D

This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for POLR2D

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA ().
Show more

Research Articles on POLR2D

1. Observational study of gene-disease association, gene-gene interaction, and gene-environment interaction. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with POLR2D recombinant protein

 Reference Product  PubMed Publications
 POLR2G recombinant protein  >2 publications with POLR2D and POLR2G

Pathways associated with POLR2D recombinant protein

 Products by Pathway  Pathway Diagram
 Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies  Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
 Activation Of HOX Genes During Differentiation Pathway antibodies  Activation Of HOX Genes During Differentiation Pathway Diagram
 Activation Of Anterior HOX Genes In Hindbrain Development During Early Embryogenesis Pathway antibodies  Activation Of Anterior HOX Genes In Hindbrain Development During Early Embryogenesis Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Of Signal Transduction Pathway antibodies  Diseases Of Signal Transduction Pathway Diagram
 Dual Incision In TC-NER Pathway antibodies  Dual Incision In TC-NER Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram

Diseases associated with POLR2D recombinant protein

 Disease Name  Pubmed Publications
 Weight Loss Antibodies  >1 publications with POLR2D and Weight Loss

Organs/Tissues associated with POLR2D recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1 publications with POLR2D and Liver
 Blood Antibodies  >1 publications with POLR2D and Blood
 Prostate Antibodies  >1 publications with POLR2D and Prostate
 Muscle Antibodies  >1 publications with POLR2D and Muscle
 Intestine Antibodies  >1 publications with POLR2D and Intestine
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000