MED20 Recombinant Protein

Name: MED20 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS204934
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204934

MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors. Recombinant human MED20 protein, fused to… More details

$230 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MED20, Recombinant Protein

Full Product Name

MED20, 1-212aa, Human, His tag, E Coli

Product Gene Name

MED20 recombinant protein

Product Synonym Names

Mediator of RNA polymerase II transcription subunit 20; PRO0213; TRFP

Sequence

MGSSHHHHHH SSGLVPRGSH MGSMGVTCVS QMPVAEGKSV QQTVELLTRK LEMLGAEKQG TFCVDCETYH TAASTLGSQG QTGKLMYVMH NSEYPLSCFA LFENGPCLIA DTNFDVLMVK LKGFFQSAKA SKIETRGTRY QYCDFLVKVG TVTMGPSARG ISVEVEYGPC VVASDCWSLL LEFLQSFLGS HTPGAPAVFG NRHDAVYGPA DTMVQYMELF NKIRKQQQVP VAGIR

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.5) containing 0.2M NaCl, 50% glycerol, 1mM DTT

Purity/Purification

> 90% by SDS-PAGE

Concentration

0.25 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

25 kDa (235aa), confirmed by MALDI-TOF

OMIM

AF097725 mRNA

3D Structure

ModBase 3D Structure for Q9H944

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for MED20 recombinant protein

Epigenomics

Applications Tested/Suitable for MED20 recombinant protein

SDS-PAGE

Product References and Citations for MED20 recombinant protein

Sato S, Tomomori-Sato C, et al. (2004). Mol Cell. 14 (5):685-91.; Zhang X, Krutchinsky A, et al. (2011). Mol Cell. 19 (1):89-100.;

Other Notes

Small volumes of MED20 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MED20 purchase

MBS204934 is a recombinant protein targeting MED20. Also known as MED20, 1-212aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for MED20. It may not necessarily be applicable to this product.

NCBI GI #

30023851

NCBI GeneID

9477

NCBI Accession #

NP_004266

UniProt Primary Accession #

Q9H944

UniProt Secondary Accession #

O95821; Q5T8J4; Q9Y429; B4DE08

UniProt Related Accession #

Q9H944

GenBank Nucleotide #

NM_004275.4

NCBI Official Full Name

mediator of RNA polymerase II transcription subunit 20 isoform 1

NCBI Official Synonym Full Names

mediator complex subunit 20

NCBI Official Symbol

MED20

NCBI Official Synonym Symbols

SRB2; TRFP; PRO0213

UniProt Protein Name

Mediator of RNA polymerase II transcription subunit 20

UniProt Synonym Protein Names

Mediator complex subunit 20; TRF-proximal protein homolog; hTRFP

Protein Family

Putative mediator of RNA polymerase II transcription

UniProt Gene Name

MED20

UniProt Synonym Gene Names

TRFP; hTRFP

NCBI Protein Information

mediator of RNA polymerase II transcription subunit 20

NCBI Summary for MED20

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]

UniProt Comments for MED20

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Research Articles on MED20

1. Our analyses revealed no apparent differences in protein expression profiles of SRBs in central and peripheral regions of human donor tissues, indicating that carotenoid-binding proteins rather than transporters are likely to mediate selective accumulation of carotenoids into the macula.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MED20 recombinant protein

Reference Product	PubMed Publications
MED18 recombinant protein	>10 publications with MED20 and MED18
MED8 recombinant protein	>7 publications with MED20 and MED8
MED6 recombinant protein	>2 publications with MED20 and MED6
MED17 recombinant protein	>2 publications with MED20 and MED17
MED15 recombinant protein	>1 publications with MED20 and MED15
MED11 recombinant protein	>1 publications with MED20 and MED11
MED7 recombinant protein	>1 publications with MED20 and MED7

Pathways associated with MED20 recombinant protein

Products by Pathway	Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies	Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Generic Transcription Pathway antibodies	Generic Transcription Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
PPARA Activates Gene Expression Pathway antibodies	PPARA Activates Gene Expression Pathway Diagram
Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway antibodies	Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway Diagram
Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies	Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram

Diseases associated with MED20 recombinant protein

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>3 publications with MED20 and Adenocarcinoma
Breast Neoplasms Antibodies	>3 publications with MED20 and Breast Neoplasms
Neoplasm Metastasis Antibodies	>1 publications with MED20 and Neoplasm Metastasis
Atrophy Antibodies	>1 publications with MED20 and Atrophy

Organs/Tissues associated with MED20 recombinant protein

Organ/Tissue Name	Pubmed Publications
Lymph Node Antibodies	>1 publications with MED20 and Lymph Node
Lymph Antibodies	>1 publications with MED20 and Lymph
Blood Antibodies	>1 publications with MED20 and Blood
Brain Antibodies	>1 publications with MED20 and Brain