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PCSK1N Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204985

Members of the subtilisin-like proprotein convertase family process latent precursor proteins into their biologically active products. PCSK1N functions as an inhibitor of prohormone convertase 1, which regulates the proteolytic cleavage of neuroendocrine… More details

$250 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PCSK1N, Recombinant Protein
Full Product Name
PCSK1N, 34-260aa, Human, His tag, E Coli (Denatured)
Product Synonym Names
ProSAAS precursor; ProSAAS precursor; Proprotein convertase subtilisin/kexin type 1 inhibitor; PROSAAS; SAAS
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMARPVKE PRGLSAASPP LAETGAPRRF RRSVPRGEAA GAVQELARAL AHLLEAERQE RARAEAQEAE DQQARVLAQL LRVWGAPRNS DPALGLDDDP DAPAAQLARA LLRARLDPAA LAAQLVPAPV PAAALRPRPP VYDDGPAGPD AEEAGDETPD VDPELLRYLL GRILAGSADS EGVAAPRRLR RAADHDVGSE LPPEGVLGAL LRVKRLETPA PQVPARRLLP P
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing, 10% glycerol, 0.4M urea
Purity/Purification
> 85% by SDS-PAGE
Concentration
0.25 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
26.6kDa (251aa)
OMIM
AF181562 mRNA
3D Structure
ModBase 3D Structure for Q9UHG2
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for PCSK1N recombinant protein

Applications Tested/Suitable for PCSK1N recombinant protein
SDS-PAGE
Application Notes for PCSK1N recombinant protein
This protein is denatured. Denatured protein is most likely not the best option for functional studies. Denatured protein is better suited for Western Blot (WB) or imaging assays. Applications are user determined.

Product References and Citations for PCSK1N recombinant protein

Fricker L., McKinzie A.A.. et al. (2000). J. Neurosci. 20:639-648

Other Notes

Small volumes of PCSK1N recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PCSK1N purchase

MBS204985 is a recombinant protein targeting PCSK1N. Also known as PCSK1N, 34-260aa, Human, His tag, E Coli (Denatured). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PCSK1N. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
proSAAS preproprotein
NCBI Official Synonym Full Names
proprotein convertase subtilisin/kexin type 1 inhibitor
NCBI Official Symbol
PCSK1N
NCBI Official Synonym Symbols
PEN; SAAS; SCG8; BigLEN; SgVIII; PROSAAS
UniProt Protein Name
ProSAAS
UniProt Synonym Protein Names
Proprotein convertase subtilisin/kexin type 1 inhibitor; Proprotein convertase 1 inhibitor
Protein Family
UniProt Gene Name
PCSK1N
UniProt Synonym Gene Names
Proprotein convertase 1 inhibitor; b-SAAS; l-SAAS; b-PEN-LEN; l-LEN; b-LEN
NCBI Protein Information
proSAAS

NCBI Summary for PCSK1N

The protein encoded by this gene functions as an inhibitor of prohormone convertase 1, which regulates the proteolytic cleavage of neuroendocrine peptide precursors. The proprotein is further processed into multiple short peptides. A polymorphism within this gene may be associated with obesity. [provided by RefSeq, Aug 2013]
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UniProt Comments for PCSK1N

May function in the control of the neuroendocrine secretory pathway. Proposed be a specific endogenous inhibitor of PCSK1. ProSAAS and Big PEN-LEN, both containing the C-terminal inhibitory domain, but not the further processed peptides reduce PCSK1 activity in the endoplasmic reticulum and Golgi. It reduces the activity of the 84 kDa form but not the autocatalytically derived 66 kDa form of PCSK1. Subsequent processing of proSAAS may eliminate the inhibition. Slows down convertase-mediated processing of proopiomelanocortin and proenkephalin. May control the intracellular timing of PCSK1 rather than its total level of activity. The function of the processed secreted peptides is not known ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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