H3F3A Recombinant Protein

Name: H3F3A Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS205022
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205022

$230 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

H3F3A, Recombinant Protein

Full Product Name

H3F3A, 1-136aa, Human, His tag, E Coli (Denatured)

Product Gene Name

H3F3A recombinant protein

Product Synonym Names

Histone H3.3; H3.3A; H3F3

Sequence

MGSSHHHHHH SSGLVPRGSH MGSMARTKQT ARKSTGGKAP RKQLATKAAR KSAPSTGGVK KPHRYRPGTV ALREIRRYQK STELLIRKLP FQRLVREIAQ DFKTDLRFQS AAIGALQEAS EAYLVGLFED TNLCAIHAKR VTIMPKDIQL ARRIRGERA

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Purity/Purification

> 90% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

17.7kDa (159aa)

OMIM

M11354 mRNA

3D Structure

ModBase 3D Structure for P84243

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for H3F3A recombinant protein

Epigenomics

Applications Tested/Suitable for H3F3A recombinant protein

SDS-PAGE

Application Notes for H3F3A recombinant protein

This protein is denatured. Denatured protein is most likely not the best option for functional studies. Denatured protein is better suited for Western Blot (WB) or imaging assays. Applications are user determined.

Product References and Citations for H3F3A recombinant protein

Tagami H., et al. (2004) Cell. 116:51-61; Daury L N., et al. (2006) EMBO Rep. 7:66-71

Other Notes

Small volumes of H3F3A recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for H3F3A purchase

MBS205022 is a recombinant protein targeting H3F3A. Also known as H3F3A, 1-136aa, Human, His tag, E Coli (Denatured). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for H3F3A. It may not necessarily be applicable to this product.

NCBI GI #

4504279

NCBI GeneID

3020

NCBI Accession #

NP_002098

UniProt Primary Accession #

P84243

UniProt Secondary Accession #

P06351; P33155; Q5VV55; Q5VV56; Q66I33; Q9V3W4

UniProt Related Accession #

P84243

GenBank Nucleotide #

NM_002107.4

NCBI Official Full Name

histone H3.3

NCBI Official Synonym Full Names

H3 histone family member 3A

NCBI Official Symbol

H3F3A

NCBI Official Synonym Symbols

H3F3; H3.3A

UniProt Protein Name

Histone H3.3

UniProt Gene Name

H3F3A

UniProt Synonym Gene Names

H3.3A; H3F3

NCBI Protein Information

histone H3.3

NCBI Summary for H3F3A

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq, Jul 2008]

UniProt Comments for H3F3A

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Research Articles on H3F3A

1. H3F3A is the most frequently mutated giant cell tumor of bone driver gene. H3F3A mutations are not present in atypical giant cell tumor of bone.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with H3F3A recombinant protein

Reference Product	PubMed Publications
H3F3B recombinant protein	>16 publications with H3F3A and H3F3B

Pathways associated with H3F3A recombinant protein

Products by Pathway	Pathway Diagram
Activated PKN1 Stimulates Transcription Of AR (androgen Receptor) Regulated Genes KLK2 And KLK3 Pathway antibodies	Activated PKN1 Stimulates Transcription Of AR (androgen Receptor) Regulated Genes KLK2 And KLK3 Pathway Diagram
Activation Of HOX Genes During Differentiation Pathway antibodies	Activation Of HOX Genes During Differentiation Pathway Diagram
Activation Of Anterior HOX Genes In Hindbrain Development During Early Embryogenesis Pathway antibodies	Activation Of Anterior HOX Genes In Hindbrain Development During Early Embryogenesis Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Amyloid Fiber Formation Pathway antibodies	Amyloid Fiber Formation Pathway Diagram
B-WICH Complex Positively Regulates RRNA Expression Pathway antibodies	B-WICH Complex Positively Regulates RRNA Expression Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Cellular Senescence Pathway antibodies	Cellular Senescence Pathway Diagram

Diseases associated with H3F3A recombinant protein

Disease Name	Pubmed Publications
Glioma Antibodies	>99 publications with H3F3A and Glioma
Nervous System Diseases Antibodies	>73 publications with H3F3A and Nervous System Diseases
Glioblastoma Antibodies	>56 publications with H3F3A and Glioblastoma
Giant Cell Tumor of Bone Antibodies	>26 publications with H3F3A and Giant Cell Tumor of Bone
Neoplasms, Experimental Antibodies	>11 publications with H3F3A and Neoplasms, Experimental
Necrosis Antibodies	>5 publications with H3F3A and Necrosis
Chromosome Aberrations Antibodies	>4 publications with H3F3A and Chromosome Aberrations
Lung Neoplasms Antibodies	>4 publications with H3F3A and Lung Neoplasms
Cell Transformation, Neoplastic Antibodies	>3 publications with H3F3A and Cell Transformation, Neoplastic
Adenocarcinoma Antibodies	>3 publications with H3F3A and Adenocarcinoma

Organs/Tissues associated with H3F3A recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>95 publications with H3F3A and Brain
Bone Antibodies	>30 publications with H3F3A and Bone
Spinal Cord Antibodies	>6 publications with H3F3A and Spinal Cord
Lung Antibodies	>6 publications with H3F3A and Lung
Muscle Antibodies	>3 publications with H3F3A and Muscle
Blood Antibodies	>3 publications with H3F3A and Blood
Embryonic Tissue Antibodies	>3 publications with H3F3A and Embryonic Tissue
Connective Tissue Antibodies	>3 publications with H3F3A and Connective Tissue
Ganglia Antibodies	>2 publications with H3F3A and Ganglia
Nerve Antibodies	>2 publications with H3F3A and Nerve