3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

NHLH1 Recombinant Protein

Name: NHLH1 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS205032
Price: 365 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205032

$365 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NHLH1, Recombinant Protein

Full Product Name

NHLH1, 1-133aa, Human, His tag, E Coli

Product Gene Name

NHLH1 recombinant protein

Product Synonym Names

Nescient helix loop helix 1; bHLHa35; HEN1; NSCL; NSCL1

Sequence

MGSSHHHHHH SSGLVPRGSH MGSMMLNSDT MELDLPPTHS ETESGFSDCG GGAGPDGAGP GGPGGGQARG PEPGEPGRKD LQHLSREERR RRRRATAKYR TAHATRERIR VEAFNLAFAE LRKLLPTLPP DKKLSKIEIL RLAICYISYL NHVLDV

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT

Purity/Purification

> 85% by SDS-PAGE

Concentration

0.25 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 2-8°C for one week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeating freeze and thawing cycles.

Molecular Weight

17.0kDa (156aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)

Sequence Positions

1-133aa

OMIM

M97507 Genomic DNA

3D Structure

ModBase 3D Structure for Q02575

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for NHLH1 recombinant protein

Epigenomics

Applications Tested/Suitable for NHLH1 recombinant protein

SDS-PAGE

Product References and Citations for NHLH1 recombinant protein

Brown L., et al. (1992) Proc. Natl. Acad. Sci. u.S.A. 89:8492-8496; Lipkowitz S., et al. (1992) J. Biol. Chem. 267:21065-21071;

Other Notes

Small volumes of NHLH1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NHLH1 purchase

MBS205032 is a recombinant protein targeting NHLH1. Also known as NHLH1, 1-133aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for NHLH1. It may not necessarily be applicable to this product.

NCBI GI #

5031943

NCBI GeneID

4807

NCBI Accession #

NP_005589

UniProt Primary Accession #

Q02575

UniProt Related Accession #

Q02575

GenBank Nucleotide #

NM_005598.3

NCBI Official Full Name

helix-loop-helix protein 1

NCBI Official Synonym Full Names

nescient helix-loop-helix 1

NCBI Official Symbol

NHLH1

NCBI Official Synonym Symbols

HEN1; NSCL; NSCL1; bHLHa35

UniProt Protein Name

Helix-loop-helix protein 1

UniProt Synonym Protein Names

Class A basic helix-loop-helix protein 35; bHLHa35; Nescient helix loop helix 1; NSCL-1

Protein Family

Helix-loop-helix protein

UniProt Gene Name

NHLH1

UniProt Synonym Gene Names

BHLHA35; HEN1; HEN-1; bHLHa35; NSCL-1

NCBI Protein Information

helix-loop-helix protein 1

NCBI Summary for NHLH1

The helix-loop-helix (HLH) proteins are a family of putative transcription factors, some of which have been shown to play an important role in growth and development of a wide variety of tissues and species. Four members of this family have been clearly implicated in tumorigenesis via their involvement in chromosomal translocations in lymphoid tumors: MYC (MIM 190080), LYL1 (MIM 151440), E2A (MIM 147141), and SCL (MIM 187040).[supplied by OMIM, Nov 2002]

UniProt Comments for NHLH1

May serve as DNA-binding protein and may be involved in the control of cell-type determination, possibly within the developing nervous system.

Research Articles on NHLH1

1. The polymorphism was further reproducibly associated with a DNA methylation signature in whole blood (P = 4.5x10-40) that also associated with allergic sensitization and expression in blood of the cytokine TSLP (P = 1.1x10-4). Knockdown of the transcription factor predicted to bind the enhancer region (NHLH1) in a human cell line (HEK293) expressing NHLH1 resulted in lower TSLP expression

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NHLH1 recombinant protein

Reference Product	PubMed Publications
SHH recombinant protein	>2 publications with NHLH1 and SHH
NEUROG1 recombinant protein	>1 publications with NHLH1 and NEUROG1
LMO2 recombinant protein	>1 publications with NHLH1 and LMO2

Diseases associated with NHLH1 recombinant protein

Disease Name	Pubmed Publications
Congenital Abnormalities Antibodies	>4 publications with NHLH1 and Congenital Abnormalities
Disease Models, Animal Antibodies	>2 publications with NHLH1 and Disease Models, Animal
Immune System Diseases Antibodies	>1 publications with NHLH1 and Immune System Diseases
Infertility, Male Antibodies	>1 publications with NHLH1 and Infertility, Male
Inflammation Antibodies	>1 publications with NHLH1 and Inflammation
Weight Loss Antibodies	>1 publications with NHLH1 and Weight Loss

Organs/Tissues associated with NHLH1 recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>14 publications with NHLH1 and Brain
Lung Antibodies	>4 publications with NHLH1 and Lung
Pituitary Gland Antibodies	>1 publications with NHLH1 and Pituitary Gland
Eye Antibodies	>1 publications with NHLH1 and Eye