GPT2 Recombinant Protein

Name: GPT2 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS205040
Price: 265 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205040

Alanine aminotransferase 2, also known as GPT2, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Subsequently, they play a key role in the intermediary metabolism of glucose and amino acids. GPT2… More details

$265 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GPT2, Recombinant Protein

Full Product Name

GPT2, 1-523aa, Human, His tag, E Coli

Product Gene Name

GPT2 recombinant protein

Product Synonym Names

Alanine aminotransferase 2; ALT2; AAT2

Sequence

MGSSHHHHHH SSGLVPRGSH MGSMQRAAAL VRRGCGPRTP SSWGRSQSSA AAEASAVLKV RPERSRRERI LTLESMNPQV KAVEYAVRGP IVLKAGEIEL ELQRGIKKPF TEVIRANIGD AQAMGQQPIT FLRQVMALCT YPNLLDSPSF PEDAKKRARR ILQACGGNSL GSYSASQGVN CIREDVAAYI TRRDGGVPAD PDNIYLTTGA SDGISTILKI LVSGGGKSRT GVMIPIPQYP LYSAVISELD AIQVNYYLDE ENCWALNVNE LRRAVQEAKD HCDPKVLCII NPGNPTGQVQ SRKCIEDVIH FAWEEKLFLL ADEVYQDNVY SPDCRFHSFK KVLYEMGPEY SSNVELASFH STSKGYMGEC GYRGGYMEVI NLHPEIKGQL VKLLSVRLCP PVSGQAAMDI VVNPPVAGEE SFEQFSREKE SVLGNLAKKA KLTEDLFNQV PGIHCNPLQG AMYAFPRIFI PAKAVEAAQA HQMAPDMFYC MKLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPVEKLKTV LQKVKDFHIN FLEKYA

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 7.5) containing 30% glycerol 0.2M NaCl, 2mM DTT

Purity/Purification

> 90% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

60.3kDa (546aa)

OMIM

AY029173 mRNA

3D Structure

ModBase 3D Structure for Q8TD30

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for GPT2 recombinant protein

Metabolism

Applications Tested/Suitable for GPT2 recombinant protein

SDS-PAGE

Product References and Citations for GPT2 recombinant protein

Sohocki M M., et al. (1997) Genomics. 40:247-252; Matthews C C., et al. (2003) Hepatology. 39: 1297-1302.

Other Notes

Small volumes of GPT2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPT2 purchase

MBS205040 is a recombinant protein targeting GPT2. Also known as GPT2, 1-523aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for GPT2. It may not necessarily be applicable to this product.

NCBI GI #

19263340

NCBI GeneID

84706

NCBI Accession #

NP_597700

UniProt Primary Accession #

Q8TD30

UniProt Secondary Accession #

Q8N9E2

UniProt Related Accession #

Q8TD30

GenBank Nucleotide #

NM_133443.3

NCBI Official Full Name

alanine aminotransferase 2 isoform 1

NCBI Official Synonym Full Names

glutamic--pyruvic transaminase 2

NCBI Official Symbol

GPT2

NCBI Official Synonym Symbols

ALT2; GPT 2; MRT49

UniProt Protein Name

Alanine aminotransferase 2

UniProt Synonym Protein Names

Glutamate pyruvate transaminase 2; GPT 2; Glutamic--alanine transaminase 2; Glutamic--pyruvic transaminase 2

Protein Family

Glucose-6-phosphate/phosphate translocator

UniProt Gene Name

GPT2

UniProt Synonym Gene Names

AAT2; ALT2; ALT2; GPT 2

NCBI Protein Information

alanine aminotransferase 2

NCBI Summary for GPT2

This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

UniProt Comments for GPT2

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.

Research Articles on GPT2

1. The GPT2 gene demonstrates increasing expression in brain in the early postnatal period, and GPT2 protein localizes to mitochondria. Akin to the human phenotype, Gpt2-null mice exhibit reduced brain growth. Through metabolomics and direct isotope tracing experiments, we find a number of metabolic abnormalities associated with loss of Gpt2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GPT2 recombinant protein

Reference Product	PubMed Publications
GOT1 recombinant protein	>4 publications with GPT2 and GOT1
GOT2 recombinant protein	>1 publications with GPT2 and GOT2
GLUD1 recombinant protein	>1 publications with GPT2 and GLUD1

Pathways associated with GPT2 recombinant protein

Products by Pathway	Pathway Diagram
2-Oxocarboxylic Acid Metabolism Pathway antibodies	2-Oxocarboxylic Acid Metabolism Pathway Diagram
2-Oxocarboxylic Acid Metabolism Pathway antibodies	2-Oxocarboxylic Acid Metabolism Pathway Diagram
Alanine, Aspartate And Glutamate Metabolism Pathway antibodies	Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
Alanine, Aspartate And Glutamate Metabolism Pathway antibodies	Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
Amino Acid Metabolism Pathway antibodies	Amino Acid Metabolism Pathway Diagram
Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies	Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
Arginine Biosynthesis Pathway antibodies	Arginine Biosynthesis Pathway Diagram
Arginine Biosynthesis Pathway antibodies	Arginine Biosynthesis Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram

Diseases associated with GPT2 recombinant protein

Disease Name	Pubmed Publications
Fatty Liver Antibodies	>3 publications with GPT2 and Fatty Liver
Nervous System Diseases Antibodies	>2 publications with GPT2 and Nervous System Diseases
Brain Diseases Antibodies	>2 publications with GPT2 and Brain Diseases
Liver Diseases Antibodies	>2 publications with GPT2 and Liver Diseases
Fetal Growth Retardation Antibodies	>1 publications with GPT2 and Fetal Growth Retardation
Lung Neoplasms Antibodies	>1 publications with GPT2 and Lung Neoplasms
Disease Models, Animal Antibodies	>1 publications with GPT2 and Disease Models, Animal
Carcinoma, Hepatocellular Antibodies	>1 publications with GPT2 and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>1 publications with GPT2 and Liver Neoplasms

Organs/Tissues associated with GPT2 recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>13 publications with GPT2 and Blood
Liver Antibodies	>8 publications with GPT2 and Liver
Brain Antibodies	>4 publications with GPT2 and Brain
Muscle Antibodies	>2 publications with GPT2 and Muscle
Adipose Tissue Antibodies	>2 publications with GPT2 and Adipose Tissue
Placenta Antibodies	>1 publications with GPT2 and Placenta
Lung Antibodies	>1 publications with GPT2 and Lung
Thyroid Antibodies	>1 publications with GPT2 and Thyroid
Embryonic Tissue Antibodies	>1 publications with GPT2 and Embryonic Tissue
Intestine Antibodies	>1 publications with GPT2 and Intestine